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Effect of Different inoculation methods on Pathogenicity of Fusarium proliferatum to Alfalfa

Asian Agricultural Research 2020年第7期12卷 64-68页

作者： Yinghua LUO Mengteng LI Congyu LI Chenghao JIN Xiangping LIU College of Animal Science and Technology Heilongjiang Bayi Agricultural University Daqing 163319 China

[Objectives]To find out the suitable inoculation method for Fusarium proliferatum.[methods]Longmu 801 and TG4 were inoculated with rice grain-based inoculum at the bud and seedling stages and with root irrigation at the seedling stage,respectively,and then,the plant height,aboveground biomass,underground biomass,incidence rate and disease index of the alfalfa were determined to conduct comprehensive resistance evaluation.[Results]There was obvious difference in disease resistance between the two alfalfa *** the three inoculation methods,the relative plant height,relative aboveground biomass and relative underground biomass of Longmu 801 differed insignificantly(P>0.05),all around *** were significant differences in TG4 among different inoculation methods(Pinoculation *** incidence rates and disease indices of Longmu 801 and TG4 inoculated with rice grain-based inoculum at the seedling stage were the lowest,and their incidence rates and disease indices were 0%and 13.30%,and 0 and 5.33,respectively,significantly lower than those under the other two inoculation methods.[Conclusions]The inoculation time and inoculation method of the pathogen both affect the inoculation *** the three inoculation methods,the inoculation with rice grain-based inoculum at the bud stage is the most pathogenic to alfalfa seedlings,and can be popularized in the resistance evaluation of alfalfa to root diseases.

关键词： Alfalfa Root rot Fusarium proliferatum inoculation method Disease resistance

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Root Plate Growth in Sunflower and Its Relevance to Sclerotinia Basal Stalk Rot

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American Journal of Plant Sciences 2022年第3期13卷 359-370页

作者： Christopher G. Misar Northern Crop Science Laboratory USDA-ARS Fargo ND USA

Sclerotinia basal stalk rot (BSR) of sunflower (Helianthus annuus L.) is a fungal disease of the roots that causes symptoms of wilt and a basal stem lesion. Evaluating root plate growth could improve our understanding of BSR. Separate studies were conducted to determine the effect of sunflower growth stage or genotype on root plate diameter in North American environments. Root plate diameter of cultivated hybrids at reproductive growth stages was 3 to 4 times larger than vegetative stages. Cultivated hybrids had larger root plate diameter than interspecific lines. These results have implications for artificial inoculation methods that evaluate genotypes for BSR resistance in the field or greenhouse. Disease escapes can occur if field-grown plants are inoculated too far from the root plate and/or too early at vegetative growth stages. Side-dressing mycelium-infested cereal grain closer (i.e., 10 cm) to plants at reproductive growth stages (i.e., R1 - R4) can increase disease pressure and reduce disease escapes. These guidelines for the field can be used to validate results from artificial inoculations in the greenhouse.

关键词： Canopy Closure inoculation method Root Plate Diameter Sclerotinia Wilt

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Construction and Application of a High-throughput method for Identifying Maize Resistance to Aspergillus flavus in the Field

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Agricultural Science & Technology 2018年第5期19卷 43-49页

作者： QI Yao-cheng DING Jian-zhou ZUO Xiao-long ZHANG Wei QIAN Yi-liang REN Si-hai WANG Jun GUO Jin RUAN Long Tobacco Research Institute Anhui Academy of Agricultural SciencesHefei 230031PRC

In order to establish a fast and effective high-throughput method for identifying maize resistance to Aspergillus flavus infection in the field,5 conidial concentrations and 3 inoculation methods were compared and optimized. A correlation model between disease levels and aflatoxin B1 accumulation was established. The results showed that inoculating maize inbred lines with the optimal conidial concentration of 1.0×10^6 c/mL could accurately show the different resistance levels of maize to *** infection,and the spiral quantitative inoculation technique could effectively avoid the defect of calculation error caused by commonly used methods. The positive correlation model of resistance level and aflatoxin B1 accumulation could effectively predict the capability of maize being resistant to ***. 8 resistant and 9 medium resistant maize inbred lines of the RIL population were *** spiral quantitative identification method realized the high-throughput identification of maize inbred lines resistant to *** infection in the field,and the results have shown high accuracy,stability and repeatability.

关键词： Maize Aspergillus flavus inoculation method Disease level Resistance evaluation

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