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infectious spondylitis-Associated Staphylococcus aureus with Virulence Gene pvl or tst Causes More Necrosis than Apoptosis in Human Alveolar Basal Epithelial Cell Line A549

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Advances in Microbiology 2016年第7期6卷 479-488页

作者： Tsung-Jen Huang Chi-Han Lee Meng-Huang Wu Yen-Yao Li Tsung Han Yang Chin-Chang Cheng Ching-Yu Lee Chih-Cheng Lu Chishih Chu Department of Orthopedic Surgery Chang Gung Memorial Hospital Chiayi Branch Taiwan College of Medicine Chang Gung University Taiwan Department of Orthopedic Surgery Taipei Medical University Hospital Taiwan School of Medicine Taipei Medical University Taiwan Department of Microbiology Immunology and Biopharmaceutics National Chiayi University Taiwan Department of Laboratory Medicine Chang Gung Memorial Hospital Linkou Branch Taiwan

Methicillin-sensitive and resistant Staphylococcus aureus (MSSA and MRSA, respectively) can cause non-tuberculosis infectious spondylitis. In 43 cases of bacterial infectious spondylitis, Mycobacterium tuberculosis and S. aureus were the two major causative pathogens. MRSA caused more anterior operations and thoracic infections, while MSSA caused more posterior infections and lumbar infections. Differences between six S. aureus isolates from infectious spondylitis were characterized. MLST and staphylococcal cassette chromosome mec (SCCmec) analysis identified MSSA ST959 and ST30 isolates, MRSA ST239/SCCmec IIIA isolates 2 and 3, ST59/SCCmec IIIA-like isolate 6, and ST30/SCCmec IV isolate 5. While all of the isolates were resistant to penicillin and ampicillin, the MRSA isolates were more resistant than the MSSA isolates. Carbapenem-resistant MRSA ST239/SCCmec IIIA and ST59/SCCmec IIIA-like isolates of the agr1 type were also resistant to clindamycin and erythromycin. Leukocidin genes (pvl or lukED) and hemolysin genes (hla, hld and hlg) were present in all of the isolates. All six isolates caused more necrosis than apoptosis in the human alveolar basal epithelial cell line A549;however, ST59/SCCmec IIIA-like isolate 6, ST30/ SCCmec IV isolate 5 with pvl genes, and MSSA ST30 isolates with tst caused greater than 40% cell death after the 4-h incubation. Regardless of the MRSA isolate and its SCCmec type or the MSSA isolate, the infectious spondylitis-associated S. aureus isolates differed genetically, and the pvl and tst genes may be important genes for cell necrosis.

关键词： infectious spondylitis Staphylococcus aureus Virulence Factor MLST Necrosis Apoptosis

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