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Phytochemical profiling and biological activity of Plectranthus amboinicus(Lour.) mediated by various solvent extracts against Aedes aegypti larvae and toxicity evaluation

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Asian Pacific Journal of Tropical Medicine 2020年第11期13卷 494-502页

作者： Deepak Paramasivam Balamuralikrishnan Balasubramanian Sungkwon Park Palanisamy Alagappan Tanushri Kaul Wenchao Liu Perumal Pachiappan Department of Biotechnology School of BiosciencesPeriyar UniversitySalemTamil Nadu-636011India Department of Biotechnology Rathnavel Subramaniam College of Arts and ScienceSulur-641402Tamil NaduIndia Department of Food Science and Biotechnology College of Life ScienceSejong UniversitySeoul-05006South Korea Nutritional Improvement of Crops International Centre for Genetic Engineering and BiotechnologyAruna Asaf Ali MargNew Delhi 110067India Department of Animal Science College of AgricultureGuangdong Ocean UniversityZhanjiangGuangdong 524088China Department of Marine Science School of Marine SciencesBharathidasan UniversityTiruchirappalli-620024Tamil NaduIndia

Objective:To analyze the phytochemical compounds and to investigate the bio-toxic efficacy of various solvent extracts of Plectranthus amboinicus against mosquito larvae activity and lethality on non-targeting ***:The methanol,ethyl acetate,hexane,and aqueous extracts of Plectranthus amboinicus were subjected to analyze the mosquitocidal activity against the dengue vector,Aedes aegypti and toxicity assays on zebra fish and brine *** replications were performed,and negative control was also ***,ethyl acetate extract of Plectranthus amboinicus was chosen for the determination of bio-active ***:The mosquitocidal assays of methanol and ethyl acetate extracts of Plectranthus amboinicus showed the maximal activity with minimal concentration against the 4 th instar mosquito-larvae of Aedes aegypti through the following lethal concentration(LC50 and LC90)values:53.36&92.51μg/m L and 13.64&86.09μg/mL,*** addition,the plant extracts showed no toxicity on zebra fish embryo and brine shrimp *** gas-chromatography analysis of the ethyl acetate extract of Plectranthus amboinicus revealed the presence of seven different *** them,PAEA-fraction 60 contained a major active bioactive compound,hexadecanoic acid,methyl ester(270.0).Conclusions:Plectranthus amboinicus possesses mosquitocidal properties and could be used as a potential alternative source for preparing the mosquitocidal agents.

关键词： Plectranthus amboinicus Cold percolation indian borage Mosquitocidal

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