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American Journal of Plant Sciences 2015年第1期6卷 181-188页

作者： Kabwe Nkongolo Kankolongo Mbuya Department of Biology Laurentian University Sudbury Canada Biomolecular Sciences Program Laurentian University Sudbury Canada National Institute for Agronomic Study and Research (INERA) Kinshasa Democratic Republic of the Congo

Several countries in Africa, Latin America along with China have incorporated QPM in their Agricultural development plan. A new quality protein maize variety (QPM) was developed by breeders and farmers using the participatory breeding approach in the DR-Congo. It is adapted to all the maize growing regions in the country. inbred lines from this new variety were produced for further development of maize synthetic populations. The main objective of the present study is to determine the level of amino acid changes in early generations of inbred lines. The results of the study revealed a significant decrease of 33% and 38% of tryptophan in S1 and S2 inbred lines compared to the original parental MUDISHI 3 population, respectively. There was a decrease of 15% of lysine in S2 inbred lines compared to the parental MUDISHI 3. Actually, S2 inbred lines of MUDISHI 3 contain similar level of lysine compared to the genetically improved normal maize (Salongo 2) that is currently released. The development of composite lines is recommended over synthetic populations to maintain the high levels of lysine and tryptophan along with other desirable agronomic characteristics since they involve the intercrossing of open pollinated varieties.

关键词： Quality Protein Maize Lysine Tryptophan Amino Acid Profile inbred Maize Synthetic Population MUDISHI 3 DR-Congo

The Current Breeding Situation and Development Strategies on High-quality inbred Rice in Guangxi

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Asian Agricultural Research 2013年第6期5卷 27-30页

作者： Guanglin LIU Yuanmeng CHEN Chuanhua CHEN Xianbin JIANG Qunchang LUO Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning Sub-center of the National Center for Rice Improvement

According to the rice quality,production and resistance of 50 high-quality inbred rice varieties bred and examined and approved in Guangxi during the period 2000-2011,we analyze the current breeding situation and existing problems concerning high-quality inbred rice va- riety in Guangxi: the rice quality has been greatly improved,but the amylose content is still low; a series of scented rice varieties with high quality and high yield have been bred,such as Zhongguangxiang No. 1,Sanxiang 628,Guixiang No. 1,Liufengxiangzhan,Jiafuxiang No. 1, Zhaoxiang No. 1; the production of high-quality inbred rice during the period 2009-2011 was significantly enhanced,annually more than 66 00 kg / ha,but the disease resistance of rice variety bred was generally poor,and disease-resistant breeding stuck in the mud. Henceforth it is necessary to be oriented by the rice market,and take the rice type with long grain,high adhesive strength,medium-low amylose content and low chalkiness degree as the breeding objectives; introduce the existing high-quality variety resources with medium amylose content at home and abroad,then directly use them or innovate upon them as hybrid strain; take the thick-and-long-grain rice type as breeding objectives,and further improve the yield per unit area of high-quality inbred rice by improving the thousand-grain weight; increase capital investment,improve breeding techniques,strengthen disease-resistant and degeneration-resistant breeding,and improve the application of modern biotechnology in the breeding of high-quality inbred rice.

关键词： High-quality inbred rice Rice quality Amylose cont

Application of Genetic Rules of Quality Traits in the Selection of Watermelon inbred Lines

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Asian Agricultural Research 2020年第3期12卷 57-59页

作者： Xiaodan LUO Shengcan HOU Zhibang HUO Zhiqiang CHENG Junlong FAN Kaifeng Academy of Agricultural and Forestry Kaifeng 475000 China Kaifeng Key Laboratory of Biological Breeding Kaifeng 475000 China

On the basis of ascertaining the dominant and recessive relationship and separation ratio of quality traits such as leaf type and peel stripes,the separation of dominant and recessive traits was studied to infer whether the genes of the previous generation were *** with homozygous recessive traits can be confirmed in F2 generation,and homozygous single lines with dominant traits can be confirmed in F3 generation,which speeds up the breeding process of watermelon inbred lines.

关键词： Watermelon inbred line Quality trait Selection

Cluster Analysis of Chinese Pumpkin inbred Lines

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Agricultural Science & Technology 2008年第4期9卷 104-107页

作者：杜晓华李小梅李新峥河南科技学院河南新乡453003

A cluster analysis was carried out based on Euclidean genetic distances through UPGMA method in Chinese pumpkin inbred lines.7 important agronomic traits of 46 Chinese pumpkin inbred lines were *** result indicated that 46 pumpkin inbred lines were clustered into 4 groups and the inter-groups distances was larger than that in *** genetic distances of parents were related to F1 performance and the results of cluster would increase effectiveness in the Chinese pumpkin crossing breeding.

关键词： Chinese pumpkin inbred lines Cluster

Cotton Recombination inbred Lines and Their Fiber Characters

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棉花学报 2002年第S1期14卷 101-101页

作者： Xiong-ming DU, Jun-ling SUN, Zhong-li ZHOU, Zhao-e PAN, Pang-bao YIN(Key Laboratory for Cotton Genetic Improvement,Ministry oj Agriculture ,China Cotton Research Institute, CAAS , Anyang 455112,China) Key Labraory for Cotton Gentic Improvement Ministry of Agriculture China Cotton Research Institute CAAS Anyang 455112China

Genetic stocks are considered to be mostimportant aspect in plant biological andmolecular *** the past,a fuzzless-lintless mutant(XZ142w)was introduced intoour group from Xuzhou Research Institute ofAgriculture Scienc... 详细信息

关键词： Cotton mutant stocks inbred length parent polymorphic varied segregation genet

Study of Yield Combining Ability and Genetic Relationship Among Exotic Tropical,Subtropical Maize(Zea mays L.) inbreds and Temperate Maize inbreds in China

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Agricultural Sciences in China 2002年第7期1卷 725-731页

作者： FAN Xing-ming, TAN Jing, YANG Jun-yun, LIU Feng, HUANG Bi-hua and HUANG Yun-xiao( Yunnan Academy of Agricultural Sciences , Kunming 650205 , P. R . China Baoshan Institute of Agricultural Sciences , Baoshan 678000 , P.R. China Dehong Institute of Agricultural Sciences , Luxi 678400 , P.R. China) Yunnan Academy of Agricultural Sciences Kunming 650205 P. R . China Baoshan Institute of Agricultural Sciences Baoshan 678000 P.R. China Dehong Institute of Agricultural Sciences Luxi 678400 P. R. China

Information on the genetic relationship between tropical maize (Zea mays L), germplasm and temperate maize germplasm is of great value to maize breeding. The objective of this study was to determine the combining ability and genetic relationship of 25 inbreds extracted from five tropical maize populations and a land race, with four temperate maize inbreds (Huangzaosi, Mol7, B73 and Dan 340). The 25 tropical inbreds were crossed with the four temperate inbreds and evaluated. Lines from Suwanl and POP28 had high general combining ability (GCA) for grain yield. The lines from POP32 (ETO) had the highest special combining ability (SCA) with B73; the average SCA value of the 5 lines was 879 kg/ha. The lines from Suwanl had the second-highest SCA (584 kg/ha) with Huangzaosi. The lines from Suwanl had the greatest relative heterosis (20%) with B73, followed by the lines from POP32 (ETO) with B73 (19%). Five heterotic patterns have been identified from this study: Suwanl × Reid, ETO × Reid, POP28× Reid, POP28× Ludahong-gu, and Suwan1× Lancaster.

关键词： Tropical maize Subtropical maize Temperate maize inbred Combining ability Heterosis Genetic relationship Special combining ability (SCA) General combining ability (GCA)

Pedigree and Germplasm Base of inbreds of the Tangshan Sipingtou Heterotic Group of Maize in China

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Agricultural Sciences in China 2003年第4期2卷 359-367页

作者： ZHENGDa-hao LIYan-ru JIShi-dong DepartmentofAgronomy AgriculturalCollegeYanbianUniversityLongjing133400P.R.China DepartmentofLifeScience NanyangNormalUniversityNanyang473061P.R.China

The Tangshan Sipingtou (TSSPT) germplasm in inbreds of the Tangshan Sipingtou heterotic group of maize in China was mainly derived from Huangzao4, and the source of TSSPT germplasm was severely restricted by the inbred Tangsipingtou. Except Tangsipingtou, the contents of TSSPT germplasm were only 50% or less in inbreds of this group, with the existence of more than 20 non-TSSPT gennplasms which resulted in abundant genetic diversity in this group. 95.22% inbreds of this group contained both TSSPT and UNS(unknown source) gennplasms in the ratio of 1 :1, with 12. 5 - 50% of each germplasm. The recombination of TSSPT and UNS germplasms had produced Huangzao4, the most important maize germplasm of compact plant form. About 90. 24% of the inbreds in this group were the inbred Huangzao4 and its derivatives. 11.11% of the derivatives contained 25 - 50% of Luda Honggu(LDHG) germplasm, and 16.67% of the derivatives contained 12. 5 - 25% Creole and 6. 25 -28.13% LSC germplasms. In addition, 97. 56% of the inbreds of this group contained one or more non-TSSPT germplasms. This indicates that the germplasm base of inbreds of the TSSPT heterotic group had changed and evolved away from actual TSSPT germplasm.

关键词： Maize Tangshan Sipingtou(TSSPT) Heterotic group inbred Germplasm

Pedigree and Germplasm Base of inbreds of the Lancaster Heterotic Group of Maize in China

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Agricultural Sciences in China 2002年第6期1卷 595-604页

作者： ZHENG Da-hao, LI Yan-ru, JIN Feng-xue and JIANG Ji-jian(Department of Agronomy, Agricultural College, Yanbian University, Jilin 133400, P.R.China) Department of Agronomy Agricultural College Yanbian University Jilin 133400 P. R. China

Lancaster Surecrop (LSC) germplasm in inbreds of the Lancaster heterotic group in China was mainly provided by the two inbreds Mol7 and Oh43. Furthermore, the source of LSC germplasm was severely restricted in two inbreds, C103 and Oh40B. The contents of LSC germplasm are 50% or less in inbreds of the group, with the existence of more than 20 non-LSC germplasms that results in the abundant genetic diversity in the group. Most inbreds of Mol7 subgroup contain 25% - 50% LSC, 12.5% - 25% Reid Yellow Dent (RYD) and 12.5%- 25% Iowa Goldmine germplasms in the ratio of 2:1:1, and most inbreds of Zi330 subgroup contain 12.5% - 25% LSC, 6.25% - 12.5% RYD, 6.25% - 12.5% Minnesota # 13 and 25% - 50% Creole germplasms in the ratio of 2:1:1 4. AH the facts indicated that the germplasm base of the Lancaster heterotic group has stupendously changed and evolved away from actual LSC germplasm.

关键词： Maize Lancaster Heterotic group inbred Germplasm base

QTL-Seq Identified a Major QTL for Grain Length and Weight in Rice Using Near Isogenic F_2 Population

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Rice science 2018年第3期25卷 121-131页

作者： QIN Yaobin CHENG Peng CHENG Yichen FENG Yue HUANG Derun HUANG Tingxu SONG Xianjun YING Jiezheng State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement China National Rice Research Institute Key Laboratory of Plant Molecular Physiology/Institute of Botany the Chinese Academy of Sciences Institute of Rice Research Fujian Academy of Agricultural Sciences/Fuzhou National Sub-Center for Rice Improvement

Mapping and isolation of quantitative trait loci(QTLs)or genes controlling grain size or weight is very important to uncover the molecular mechanisms of seed development and crop *** identify the QTLs controlling grain size and weight,we developed a near isogenic line F_2(NIL-F_2)population,which was derived from a residual heterozygous plant in an F_7 generation of recombinant inbred line(RIL).With the completion of more than 30×whole genome re-sequencing of the parents,two DNA bulks for large and small grains,a total of 58.94 Gb clean nucleotide data were generated.A total of455 262 single nucleotide polymorphisms(SNPs)between the parents were identified to perform bulked QTL-seq.A candidate genomic region containing SNPs strongly associated with grain length and weight was identified from 15 to 20 Mb on chromosome *** designated the major QTL in the candidate region as q ***,q TGW5.3 was further validated with PCR-based conventional QTL mapping method through developing simple sequence repeat and Insertion/Deletion markers in the F_2 ***,recombinants and the progeny tests delimited the candidate region of q TGW5.3 to 1.13 Mb,flanked by HX5009(15.15 Mb)and HX5003(16.28 Mb).A set of NILs,selected from the F_2 population,was developed to evaluate the genetic effect of q *** QTL effects were detected on grain length,grain width and 1000-grain weight of H12-29 allele with 1.14 mm,-0.11 mm and 3.11 g,which explained 99.64%,95.51%and 97.32%of the phenotypic variations,respectively.

关键词： grain length grain weight QTL-seq quantitative trait locus near-isogenic line rice single nucleotide polymorphism recombinant inbred line qTGW5.3

Identification of QTLs and Validation of qCd-2 Associated with Grain Cadmium Concentrations in Rice

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Rice science 2019年第1期26卷 42-49页

作者： LIU Wenqiang PAN Xiaowu LI Yongchao DUAN Yonghong MIN Jun LIU Sanxiong LIU Licheng SHENG Xinnian LI Xiaoxiang Hunan Rice Research Institute Hunan Academy of Agricultural Sciences/Key Laboratory of Indica Rice Genetics and Breeding in the Middle and Lower Reaches of Yangtze River Valley Ministry of Agriculture

Cadmium(Cd) is one of heavy metals harmful to human health. As rice is the main staple food in Asia and Cd is easily contaminated in rice, the molecular regulation of Cd accumulation should be explored. In this study, a recombinant inbred population derived from Xiang 743/Katy was grown in Cd-polluted fields and used to map the quantitative trait loci(QTLs) for Cd accumulation in rice grains. We identified seven QTLs distributed on chromosomes 2, 3, 6, 7, 8 and 10. These QTLs displayed phenotypic variances of 58.50% and 40.59% in 2014 and 2015, respectively. Two QTLs, qCd-2 and qCd-7, were identified in both the two years. qCd-2 was detected on the interval of RM250–RM207 on chromosome 2, with an LOD of 2.51 and a phenotypic contribution of 13.75% in 2014, and an LOD of 3.35 and a phenotypic contribution of 14.16% in 2015. qCd-7 co-localized with the cloned qCdT7 on chromosome 7 and may represent the correct candidate. The other five QTLs were detected only in one year. To further confirm the effects of qCd-2, a residual heterozygous line designated as RHL945, with a heterozygous interval of RM263–RM207 on chromosome 2, was selected from the recombinant inbred population and used to develop an F2 population consisting of 155 individual plants. By incorporating further simple sequence repeat markers into the segmental linkage map of the target region, qCd-2 was delimited in the interval of RM5404–RM3774, with an LOD value of 4.38 and a phenotypic contribution of 15.52%. These results reflected the genetic regulation of grain Cd in rice and paved the way for the future cloning of qCd-2.

关键词： cadmium recombinant inbred line quantitative trait locus rice simple sequence repeat