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Identification and QTL mapping of Z550, a rice backcrossed inbred line with increased grains per panicle

Journal of Integrative Agriculture 2019年第3期18卷 526-531页

作者： WANG Shi-ming CUI Guo-qing WANG Hui MA Fu-ying XIA Sai-sai LI Yun-feng YANG Zheng-lin LING Ying-hua ZHANG Chang-wei HE Guang-hua ZHAO Fang-ming Rice Research Institute Academy of Agricultural SciencesSouthwest UniversityChongqing 400715P.R.China

An elite backcrossed inbred line Z550 with increased grains per panicle was identified from advanced backcrosses between Nipponbare and Xihui 18 by simple sequence repeat(SSR) marker-assisted selection(MAS). Z550 carries 13 substitution segments distributed on chromosomes 1, 6, 7, 8, 9, 10, and 12, with an average substitution length of 1.68 Mb. Compared with the Nipponbare parental line, plant height, panicle length, spikelets per panicle, grains per panicle, and grain weight for Z550 were significantly increased. While the grain width of Z550 was significantly narrower, and the seed setting ratio(81.43%) was significantly lower than that of Nipponbare, it is still sufficient for breeding purposes. Quantitative trait loci(QTLs) mapping for important agronomic traits was conducted with the F_2 population derived from Nipponbare crossed with Z550 using the restricted maximum likelihood(REML) method. A total of 16, including 12 previously unreported QTLs were detected, with contribution rates ranging from 1.46 to 10.49%. Grains per panicle was controlled by 8 QTLs, 5 of which increased number of grains whereas 3 decreased it. qGPP-1, with the largest contribution(10.49%), was estimated to increase grains per panicle by 30.67, while q GPP-9, with the minimum contribution rate(2.47%), had an effect of increasing grains per panicle by 15.79. These results will be useful for further development of single segment substitution lines with major QTLs, and for research of their molecular functions via QTL cloning.

关键词： rice backcrossed inbred line yield QTL mapping

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Identification of Stable Quantitative Trait Loci for Sheath Blight Resistance Using Recombinant inbred Line

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Rice science 2019年第5期26卷 331-338页

作者： CHEN Yuan ZENG Yuxiang JI Zhijuan LIANG Yan WEN Zhihua YANG Changdeng State Key Laboratory of Rice Biology China National Rice Research Institute

To identify stable quantitative trait loci(QTLs)responsible for sheath blight resistance,a recombinant inbred line mapping population consisting of 219 lines was developed by crossing Lemont and Yangdao *** disease rating,average lesion length,maximum disease rating and maximum lesion length were assayed in six different environments.A total of 128 minor effect QTLs were detected by multiple interval *** QTLs explained less than 11.2%of the phenotypic variations individually,and 106 QTLs were clustered in 20 QTL-rich regions/putative *** QTL×environment interactions were detected at three putative loci(qSBR11.1,qSBR11.2 and qSBR11.3),indicating that these three loci were not *** other 17 stable loci(qSBR1.1,qSBR1.2,qSBR2.1,qSBR2.3,qSBR3.1,qSBR3.2,qSBR3.5,qSBR3.6,qSBR5.1,qSBR7.1,qSBR8.1,qSBR9.1,qSBR9.2,qSBR9.3,qSBR12.1,qSBR12.2 and qSBR12.4)provided a foundation for marker-assisted selection in *** of allelic effect on the 20 putative loci identified 7 highly stable loci,including qSBR3.2,qSBR7.1,qSBR8.1,qSBR9.2,qSBR9.3,qSBR12.1 and qSBR12.2.

关键词： rice sheath blight resistance quantitative trait locus recombinant inbred line

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Establishment of a genetic transformation system for maize inbred P9-10

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Chinese Science Bulletin 1999年第7期44卷 624-627页

作者： Fengyong Zhou Guoying Wang Youju Xie Hongzhi Cui Sandui Guo Jingruil Dai China Agr Univ State Key Lab Agrobiotechnol Beijing 100094 Peoples R China China Agr Univ Coll Plant Sci & Technol Beijing 100094 Peoples R China Chinese Acad Agr Sci Ctr Biotechnol Beijing 100081 Peoples R China

It was found that the supplement of 10-4 mol/L AgNO3 in N6 medium enhanced the induction of type Ⅰembryogenic calli from immature embryos of maize inbred P9-10. After high-osmotic treatment, the induced calli was taken as transformation recipient to be bombarded with plasmid pMG6 carrying synthetic Bt gene by PDS-1000/He genegun. A total of 14 resistant calli were obtained after screening subsequently on the mediums with gradually increasing selective pressure. 10 plants were regenerated from the resistant calli on 3 different induction media,and 8 out of the 10 regenerated plants were confirmed to be integrated with Bt gene by PCR and Southern blotting analysis. Results of ELISA showed that the Bt-protein content in the leaves of the transgenic plants varied between 20—200 ng/g fresh weight.

关键词： maize inbred genetic transformation Bt gene.

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ESTABLISHMENT AND INVESTIGATION OF AN IL-2 DEPENDENT,THY1,2^+ SPLENIC T-CELL LINE FROM inbred 615 STRAIN MICE

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Chinese Science Bulletin 1990年第10期35卷 879-880页

作者：程一櫂王明俭许广源顾寿智钱振超 Dalian Medical College Dalian 116023 PRC

The 615 mouse is an inbred strain mouse cultivated by Institute of Haematology of the Chinese Academy of Medical Sciences. It has been widely used in China and internationally registered.

关键词： inbred cultivated registered CELL chromosome hasbeen ConA isolation suspension passage

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Identification of QTLs for Blast, Bacterial Blight, and Planthopper Resistance Using SNP-Based Linkage Maps from Two Recombinant inbred Rice Lines

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American Journal of Plant Sciences 2019年第5期10卷 760-779页

作者： Jirapong Jairin Phanchita Vejchasarn Thanapa Somjai Kanuengnij Srivilai Kulchana Darwell Phikul Leelagud Rungnapa Kawichai Jate Kotcharerk Arissara Suthanthangjai Nattaya Popa Suphalaksana Lachanthuek Varapong Chamarerk Ubon Ratchathani Rice Research Center Mueang Ubon Ratchathani Thailand Sakon Nakhon Rice Research Center Mueang Sakon Nakhon Thailand Phrae Rice Research Center Mueang Phrae Thailand Phitsanulok Rice Research Center Wang Thong Phitsanulok Thailand GenePlus Co. Ltd. Huai Khwang Bangkok Thailand Rice Department Chatuchak Bangkok Thailand

Rice is the most significant global food security. Several biotic factors limit rice production, breeding biotic-resistant rice has, therefore, become an increasingly important goal. Two elite rice lines, IR71033-121-15 (IR71033) and IR57514-PMI-5-B-1-2 (IR57514), provide potential genes for biotic stress resistance traits. In this study, genotyping by sequencing (GBS) for single nucleotide polymorphism (SNP)-based linkage map construction was used to detect quantitative trait loci (QTLs) for blast (BL), bacterial blight (BB), whitebacked planthopper (WBPH), and brown planthopper (BPH) resistance. IR71033 was derived from Oryza minuta and carried BL, BB, WBPH, and BPH resistance QTLs. IR57514 is a well-adapted rainfed lowland line that carries BL and BB resistance QTLs. Two sets of recombinant inbred line (RIL) populations derived from crosses of KDML105 × IR71033 and KDML105 × IR57514 were used to dissect the genetic basis of disease and insect pest resistance. The RIL populations were evaluated for BL, BB, WBPH, and BPH resistance from 2016 to 2018 at four rice research centers in Thailand. From these, we identified a large number of SNPs through GBS and constructed high-resolution linkage maps. By combining phenotypic evaluation with the GBS data, a total of 24 QTLs on four chromosomes were detected that confered pest resistance and explained 7.3% - 61.4% of the phenotypic variance. These findings should facilitate identifying novel resistance genes and applying marker-assisted selection for resistance to the four major rice pests investigated here. These strategies will improve the resilience and reliability of rice varieties adapted to the low-yielding environment of rainfed lowland areas worldwide.

关键词： Recombinant inbred Line Genotyping by Sequencing Resistance Gene Rainfed Lowland Rice Single Nucleotide Polymorphism Quantitative Trait Locus

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Evaluation of Sponge Gourd(Luffa cylindrical L.)inbred Lines for Growth Potential and Fruit Quality in Thua Thien Hue Province,Central Vietnam

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Journal of Agricultural Science and Technology(A) 2017年第B10期7卷 10-16页

作者： Hai Thi Hong Truong Thao Thu Phan The Thi Dieu Nguyen Agronomy Faculty University of Agriculture and ForestryHue University102 Phung Hung StreetHue 530000Vietnam Institute of Biotechnology Hue UniversityPhu ThuongPhu VangThua Thien Hue 530000Vietnam Hue University 3 Le Loi StreetHue 530000Vietnam

Plants which contain unisexual flowers are subjected to genetic degradation and yield capacity reduction due to the easily *** gourd,one of species of the Cucurbitaceae which is predominantly cross-pollinating,is cultivated to widely use as vegetable in Vietnamese daily *** aim of this experiment was to evaluate the growth potential and fruit quality including fruit aroma of sponge gourd inbred *** line selection has been performed using self-pollinated method,so that sponge gourd lines would be recovered with high yielding and aroma and thereby recommended for further crop system in Thua Thien Hue province,Central *** experiment was conducted in a randomized complete block design(RCBD)with three replications during the winter-spring season in *** data were observed and analyzed on seven inbred lines(1-MN1,1-MN2,5-MN1,7-MN1,13-MN2,15-MN1 and 15-MN2)derived from B29 accession(GBVN006904).The results indicated that inbred lines 13-MN2,1-MN2 and 15-MN1 attained more highly fruit weight and yield compared to the *** inbred lines consisting of 5-MN1,7-MN1,15-MN1 and 15-MN2 still retained the light aroma after *** inbred lines 1-MN2,7-MN1,13-MN2 and 15-MN1 are considered for appropriately using in breeding program of new luffa.

关键词： Sponge gourd inbred line growth potential fruit aroma Thua Thien Hue

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Differential Expression of microRNAs in Maize inbred and Hybrid Lines during Salt and Drought Stress

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American Journal of Plant Sciences 2010年第2期1卷 69-76页

作者： Yeqin Kong Axel A. Elling Beibei Chen Xingwang Deng 不详

Here, we analyzed whether the microRNA (miRNA) expression levels differ between maize inbred lines B73 and Mo17 and their reciprocal hybrids under salt and drought stress. We found that miR156, miR164, miR166, miR168, miR171 and miR319 are differentially expressed under abiotic stress. Interestingly, Mo17 × B73 showed the strongest change in miRNA expression in response to salt or drought stress, and was also the most resilient line when under abiotic stress in terms of water loss. In summary, our findings open the possibility that differential miRNA expression levels might be involved in heightened stress tolerance in maize hybrids.

关键词： microRNA Maize Hybrid inbred Salt Stress Drought

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Comparative evaluation of maize inbred lines (zea mays l.) according to dus testing using morphological, physiological and molecular markers

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Agricultural Sciences 2010年第3期1卷 131-142页

作者： Vinod Kumar Yadav Indra Sen Singh 不详

A major challenge facing those involved in the testing of new plant varieties for Distinctness, Uniformity and Stability (DUS) is the need to compare them against all those of 'common knowledge'. A set of maize inbred lines was used to compare how morphological, physiological characterization and RAPD molecular marker described variety relationships. All the inbred lines were confirmed as morphologically and physiologically distinct. At morphological level the maximum genetic distance (10.8) and least genetic distance (1.6) were found. For physiological characters distance varied from 0.35 to 1.92 and results from dendrogram, which was made on the basis of dissimilarity matrix, were grouped into five major clusters. From RAPD, random primers provide polymorphic amplification products;the distance varying 0.42 to 0.65 and dendrogram showed that these lines formed close clusters due to the less variation in these lines at molecular level. In the present study, the molecular markers also exposed useful genetic diversity and the visual displays appeared to disperse the lines somewhat more evenly over the plot than the morphological and physiological methods.

关键词： DUS inbred Lines Maize Markers Genetic Distance Diversity

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Adaptability and recovery capability of two maize inbred-line foundation genotypes, following treatment with progressive water-deficit stress and stress recovery

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Agricultural Sciences 2013年第8期4卷 389-398页

作者： Xianwei Fan Guiyuan Huang Liujun Zhang Tao Deng Youzhi Li State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi University Nanning China

Two maize inbred lines, the foundation genotype Y478 and its derived line Z58, are widely used to breed novel maize cultivars in China, but little is known about which traits confer Z58 with superior drought tolerance and yield. In the present study, responses in growth traits, photosynthetic parameters, chlorophyll fluorescence and leaf micromorphological characteristics were evaluated in Y478 and Z58 subjected to water-deficit stress induced by PEG 6000. The derived line Z58 showed greater drought tolerance than Y478, which was associated with higher leaf relative water content (RWC), root efficiency, and strong growth recovery. Z58 showed a higher stomatal density and stomatal area under the non-stressed condition;in these traits, both genotypes showed a similar decreasing trend with increased severity of water-deficit stress. In addition, the stomatal size of Y478 declined significantly. These micromorphological differences between the two lines were consistent with changes in physiological parameters, which may contribute to the enhanced capability for growth recovery in Z58. A non-linear response of Fv/Fm to leaf RWC was observed, and Fv/Fm decreased rapidly with a further gradual decline of leaf RWC. The relationship between other chlorophyll fluorescence parameters (photochemical quenching and electron transport rate) and RWC is also discussed.

关键词： Maize inbred Lines Morphological Response Gas Exchange Chlorophyll Fluorescence Water-Deficit Stress Recovery

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Evaluation of Reproductive Characteristics of 21 Highly inbred Lines of White Leghorns Divergently Selected for or Segregating in Tumor Resistance

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Open Journal of Animal Sciences 2015年第1期5卷 59-70页

作者： Gururaj Kulkarni Huanmin Zhang USDA Agriculture Research Service Avian Disease and Oncology Laboratory East Lansing USA

Reproduction performance of 21 inbred experimental lines of White Leghorns was evaluated based on samples of reproduction records over a period of eight consecutive years. Two lines (63 and 72) have been extensively used in studies, especially in research seeking for genetic and epigenetic factors underlying resistance to avian tumor virus-induced diseases in chickens. The other 19 lines are recombinant congenic strains (RCS), which were generated by crossing lines 63 and 72 followed by two consecutive backcrosses to the line 63 and then full-sib mating. In theory, each RCS processes 7/8 of progenitor background line 63 genome and a random sample (1/8) of the progenitor donor line 72 genome. All 21 inbred lines share a common major histocompatibility complex haplotype, B*2. The estimated average fertility of the 21 inbred lines ranged from 72.9% (RCS-J) up to 96.8% (RCS-P). Both progenitor lines 63 and 72 were observed with lower average fertility (82.4% and 81.6%, respectively) in comparison with the RCS except the RCS-J, suggesting a substantial polygenic component underlying the fertility phenotype. The average embryo mortality rate ranged from 14.5% (RCS-P) up to 47.0% (RCS-M). The background line 63 fell at about the middle of the range (28.3%) significantly higher than the donor line 72 (15.7%), which was among the group with the lowest embryo mortality. By definition, hatchability of fertile eggs is reversely correlated with embryo mortality. The average hatchability ranged from 26.5% (RCS-M) up to 66.8% (line 72) while the background line 63 remained (46.6%) at about the middle of the range. The variability of the average embryo mortality and hatchability observed among the 21 inbred lines indicated the two correlated traits also follow polygenic models of inheritance. Findings from this study paves the way for further investigation on genetic and environmental influence over reproductive performance of inbred lines of chickens, and particularly in understan

关键词： White Leghorns inbred Lines Recobinant Congenic Strains Fertility Embryo Mortality Hatchabiity Polygentic Inheritance

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