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Buffalo anti-PDC-109 antibodies improve the semen quality profiles and in-vitro zona binding index and minimize the cryoinjury of sperm in cryopreserved buffalo semen

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Asian pacific Journal of Reproduction 2023年第3期12卷 131-143页

作者： S.S.Ramteke J.S.Rajoriya A.M.Shende S.K.Ghosh J.K.Prasad P.Perumal MAFSU-College of Veterinary and Animal Sciences UdgirMaharashtraIndia NDVSU-College of Veterinary Science and Animal Husbandry RewaMadhya PradeshIndia MAFSU-Nagpur Veterinary College NagpurMaharashtraIndia ICAR-Indian Veterinary Research Institute IzatnagarUttar PradeshIndia ICAR-Central Island Agricultural Research Institute Port BlairAndaman and Nicobar IslandsIndia

Objective:To optimize the concentration of PDC-109 protein in semen for higher cryopreservability and fertility by sequestration or neutralization of PDC-109 by its antibodies(anti-PDC-109 antibodies)in bubaline species.Methods:PDC-109 protein was purified by applying two-step chromatography procedures.Purified protein was injected in rabbits to raise antibodies.These raised anti-PDC-109 antibodies were used in neutralization or sequestration of PDC-109 in in-vitro model.Ejaculates were collected from buffaloes and splited for four groups.Group 1 received egg yolk Tris glycerol extender,without anti-PDC-109 antibodies,while group 2 to 4 received anti-PDC-109 antibodies 266μg/mL,80μg/mL,and 26μg/mL in Tris-fructose-citrate buffer,respectively.Semen quality parameters viz.,forward progressive motility,viability,total morphological abnormality,acrosomal integrity,plasma membrane integrity,cryoinjury and in-vitro zona binding index were evaluated.Results:Semen quality parameters of neat semen were within the normal range of bubaline species.Sperm motility,livability,acrosomal integrity,plasma membrane integrity,and cholesterol content of sperm were decreased and total sperm abnormality was increased significantly in post-thaw semen compared to those in pre-freeze and fresh semen(Pvitro zona binding index with simultaneously reducing cryoinjury or cryodamage in the sperm of bubaline species.

关键词： Anti-PDC-109 antibodies Buffalo in-vitro capacitation In-vitro fertilization Semen

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