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Polyurethane as substrate for fungal strains

Advances in Bioscience and Biotechnology 2011年第2期2卷 52-58页

作者： Araceli Loredo-Trevino Gilberto Garcia Abraham Velasco-Tellez Raul Rodriguez-Herrera Cristobal N.Aguilar Group of Bioprocesses.School of Chemistry.University of Coahuila.Saltillo Mexico Research and Development Division.Nemak SA GarciaN.L.Mexico Biotechnology Department.School of Chemistry.University of Coahuila.Saltillo Mexico

Polyurethane is a versatile plastic with several industrial applications in the modern life, but it is considered as a very recalcitrant material. Biodegradation of this plastic has been poorly explored, and most of the studies that have been published focus on bacterial enzymes. In this work, some fungi with the capacity of growing with polyurethane foam as nutrient source were isolated from sands contaminated with this plastic and from DIA/UAdeC collection, testing their ability to grow on polyurethane as sole carbon and nitrogen sources and their enzymatic activities were determined in specific media as well as their invasion capacity on polyurethane agar plates. 22 fungal strains demonstrated their capacity of growing on polyurethane. Among the enzymatic activities evaluate, the most common was the urease activity (95% of the strains).Protease, esterase and laccase activities were present in 86%, 50% and 36% respectively. The great ability of the isolated fungal strains to use polyurethane foam as nutrient opens an important opportunity to study at detail the biodegradation of this plastic, with clear implications in cell biology and environmental technology.

关键词： Polyurethane Biodegradation Fungal Enzymes in-plate screening

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