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Implantable optical fiber microelectrode with anti-biofouling ability for in vivo photoelectrochemical analysis

Chinese Chemical Letters 2023年第3期34卷 259-263页

作者： Lixia Tao Yao Kong Yunhui Xiang Yu Cao Xiaoxue Ye Zhihong Liu College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072China Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules and College of Chemistry and Chemical Engineering Wuhan 430072China

In-situ monitoring of neurochemicals is of vital importance for the understanding of brain ***-based photoelectrochemical(PEC) sensing has emerged as a promising tool for in vivo analysis since it inherits the merits of both optical and electrochemical methods. However, the in-situ excitation of photoactive materials on the photoelectrode in living body is still a challenge because of limited tissue penetration depth of light. To circumvent this problem, we herein developed an implantable optical fiber(OF)-based microelectrode for in vivo PEC analysis. The working electrode was constructed by coating Au film as conducting layer and CdS@ZnO as photoactive material on a micron-sized OF,which was free of the limitation of light penetration in biological tissues. Further decoration of an antibiofouling layer on the surface made the sensor robust in biosamples. It was successfully applied for monitoring Cu^(2+) level in three different brain regions in the rat model of cerebral ischemia/reperfusion.

关键词： Optical fiber Implantable microelectrode Photoelectrochemical sensing in vivo analysis Neurochemicals

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Single-atom electrocatalysis: a new approach to in vivo electrochemical biosensing

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Science China Chemistry 2019年第12期62卷 1720-1724页

作者： Hanfeng Hou Junjie Mao Yunhu Han Fei Wu Meining Zhang Dingsheng Wang Lanqun Mao Yadong Li Department of Chemistry Renmin University of ChinaBeijing 100872China Beijing National Laboratory for Molecular Sciences Key Laboratory of Analytical Chemistry for Living BiosystemsInstitute of ChemistryChinese Academy of SciencesBeijing 100190China Department of Chemistry Tsinghua UniversityBeijing 100084China Key Laboratory of Functional Molecular Solids Ministry of EducationAnhui Key Laboratory of Molecule-Based MaterialsCollege of Chemistry and Materials ScienceAnhui Normal UniversityWuhu 241000China

Modulation of interfacial electron transfer has been proven to pave a new approach to in vivo electrochemical monitoring of brain chemistry;however,designing and establishing highly efficient electrocatalytic scheme towards neurochemicals remain a longstanding ***,we find that recently established single-atom catalyst(SAC)can be used for catalyzing the electrochemical process of physiologically relevant chemicals and thus offers a new avenue to in vivo electrochemical *** prove this new concept,we used Co single-atom catalyst(Co-SAC),in which the atomic active sites are dispersed in ordered porous N-doping carbon matrix at atomic level,as an example of SACs for analyzing glucose as the physiologically relevant model *** found that Co-SAC catalyzes the electrochemical oxidation of hydrogen peroxide(H2O2)at a low potential of ca.+0.05 V(***/AgCl).This property was further used for developing an oxidase-based glucose biosensor that was used subsequently as a selective detector of an online electrochemical system(OECS)for continuous monitoring of microdialysate glucose in rat *** OECS with Co-SAC-based glucose biosensor as the online detector was well responsive to glucose without interference from other electroactive species in brain *** study essentially offers a new approach to in vivo electrochemical analysis with SACs as electrocatalysts to modulate interfacial electron transfer.

关键词： single-atom catalyst in vivo analysis electrocatalysis biosensor

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3D visualization and volume-based quantification of rice chalkiness in vivo by using high resolution micro-CT

3D visualization and volume-based quantification of rice cha...

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第十九届中国作物学会学术年会

作者： Yi Su Lang-Tao Xiao College of Bioscience and Biotechnology Hunan Agricultural University

Rice quality research attracts attention worldwide. Rice chalkiness is one of the key indexes determining rice kernel quality. The traditional rice chalkiness measurement methods only use milled rice as materials and are mainly based on naked-eye observation or area-based two-dimensional(2 D) image analysis and the results could not represent the three-dimensional(3 D) characteristics of chalkiness in the rice kernel. These methods are neither in vivo thus are unable to analyze living rice seeds for high throughput screening of rice chalkiness phenotype. Here, we introduced a novel method for 3 D visualization and accurate volume-based quantification of rice chalkiness in vivo by using X-ray microcomputed tomography(micro-CT). This approach not only develops a novel volume-based method to measure the 3 D rice chalkiness index, but also provides a high throughput solution for rice chalkiness phenotype analysis by using living rice seeds. Our method could be a new powerful tool for rice chalkiness measurement, especially for high throughput chalkiness phenotype screening using living rice seeds. This method could be used in chalkiness phenotype identification and screening, and would greatly promote the basic research in rice chalkiness regulation as well as the quality evaluation in rice production practice.

关键词： rice chalkiness 3D visualization volume based quantification micro-CT in vivo analysis

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基于电喷雾萃取电离质谱分析的原位活体检测技术

基于电喷雾萃取电离质谱分析的原位活体检测技术

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中国有机质谱学第十四届全国学术大会

作者：陈焕文丁建华任玉林费强吉林大学化学院

质谱法具有高灵敏度,高特异性和适用范围广等特点。很多年前,人们就对未来小型质谱仪在诊所和太空中的应用做了科学预测。然而,由于传统的质谱分析需要复杂的样品预处理过程,究竟如何才能够方便快捷而且科学地将质谱仪应用到疾病诊断中... 详细信息

质谱法具有高灵敏度,高特异性和适用范围广等特点。很多年前,人们就对未来小型质谱仪在诊所和太空中的应用做了科学预测。然而,由于传统的质谱分析需要复杂的样品预处理过程,究竟如何才能够方便快捷而且科学地将质谱仪应用到疾病诊断中呢,直到电喷雾萃取电离(EESI)技

关键词： EESI in vivo analysis biological sample clinic diagnosis explosives

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