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Microfluidic thermotaxic selection of highlymotile sperm and in vitro fertilization

Bio-Design and Manufacturing 2024年第5期7卷 687-700页

作者： Sihan Chen Jiemin Chen Zihan Qin Jibo Wang Yuwen Wang Rong Liu Wen Zhao Ming Zhang Yuanzhen Zhang Mengcheng Luo Pu Chen Tissue Engineering and Organ Manufacturing(TEOM)Lab Department of Biomedical EngineeringTaiKang Medical School(School of Basic Medical Sciences)Wuhan UniversityWuhan 430071China Hubei Provincial Key Laboratory of Developmentally Originated Disease TaiKangMedical School(School of Basic Medical Sciences)Wuhan UniversityWuhan 430071China Reproductive Medicine Center Zhongnan Hospital of Wuhan UniversityWuhan 430071China TaiKang Center for Life and Medical Sciences Wuhan UniversityWuhan 430071China

The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization(IVF)and normal embryonic *** adopted clinical approaches for sperm sample processing intensely rely on centrifugation and wash steps that may induce mechanical damage and oxidative stress to *** a few microfluidic sperm sorting devices may avoid these adverse effects by exploiting intrinsic guidance mechanisms of sperm swimming,none of these approaches have been fully validated by clinical-grade assessment *** this study,a microfluidic sperm sorting device that enables the selection of highly motile and functional sperm via their intrinsic thermotaxis is *** by the temperature microenvironment in the fallopian tube during natural sperm selection,a microfluidic device with controllable temperature gradients along the sperm separation channel was designed and *** study investigated the optimal temperature conditions for human sperm selection and fully characterized thermotaxis-selected sperm with 45 human sperm *** indicated that a temperature range of 35–36.5℃along the separation channel significantly improves human sperm motility rate((85.25±6.28)%vs.(60.72±1.37)%;P=0.0484),increases normal sperm morphology rate((16.42±1.43)%vs.(12.55±0.88)%;Pfertilization rate and improved embryonic development from zygote to *** microfluidic thermotaxic selection approach will be translated into clinical practice to improve the IVF success rate for patients with oligozoospermia and asthenozoospermia.

关键词： Microfluidic device Thermotaxis Sperm sorting Assisted reproductive technology in vitro fertilization

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Association between Exposure of Rare Earth Elements and Outcomes of in vitro fertilization-Embryo Transfer in Beijing

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Biomedical and Environmental Sciences 2024年第8期37卷 876-886页

作者： Yutong Wang Jing Li Shirong Xu Shengli Lin Zhenchen Hou Linlin Wang Yali Huang Yue Sun Wei Guo Lailai Yan Ying Wang Chan Tian State Key Laboratory of Female Fertility Promotion Center for Reproductive MedicineDepartment of Obstetrics and Gynecology Peking University Third HospitalBeijing 100191China Department of Medical Genetics Peking UniversityBeijing 100191China National Clinical Research Center for Obstetrics and Gynecology Peking University Third HospitalBeijing 100191China Key Laboratory of Assisted Reproduction(Peking University) Ministry of EducationBeijing 100191China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology Beijing 100191China Central Laboratory of School of Public Health Peking UniversityBeijing 100191China School of Basic Medical Sciences Peking UniversityBeijing 100191China

Objective The study aimed to investigate the impact of rare earth elements(REEs)exposure on pregnancy outcomes of in vitro fertilization-embryo transfer(IVF-ET)by analyzing samples from *** A total of 141 couples were *** and follicular fluid from the wives and semen plasma from the husbands,were analyzed for REEs using inductively coupled plasma mass spectrometry(ICP-MS).Spearman's correlation coefficients and the Mann–Whitney U test were used to assess correlations and compare REE concentrations among three types of samples,*** models were utilized to estimate the individual REE effect on IVF-ET outcomes,while BKMR and WQS models explored the mixture of REE interaction effects on IVF-ET *** Higher La concentration in semen(median 0.089 ng/mL,P=0.03)was associated with a lower fertilization ***,this effect was not observed after artificial selection intervention through intracytoplasmic sperm injection(ICSI)(P=0.27).In semen,the REEs mixture did not exhibit any significant association with clinical *** Our study revealed a potential association between high La exposure in semen and a decline in fertilization rate,but not clinical pregnancy *** is the first to report REEs concentrations in follicular fluid with La,Ce,Pr,and Nd found at significantly lower concentrations than in serum,suggesting that these four REEs may not accumulate in the female reproductive ***,at the current exposure levels,mixed REEs exposure did not exhibit reproductive toxicity.

关键词： Rare earth elements in vitro fertilization Pregnancy outcomes Mixture exposure analysis

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Effect of Acupuncture on Endometrial Blood Flow in Women Undergoing in vitro fertilization Embryo Transfer: A Single Blind, Randomized Controlled Trial

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Chinese Journal of Integrative Medicine 2024年第1期30卷 10-17页

作者： DONG Hao-xu Wu Xiao ZHOU Qin HUANG Dong-mei ZHANG Ming-min HUANG Guang-ying YANG Wei Department of Integrated Traditional Chinese and Western Medicine Tongji HospitalHuazhong University of Science and TechnologyWuhan(430030)China Department of Obstetrics and Gynecoiogy Tongji HospitalHuazhong University of Science and TechnologyWuhan(430030)China Institute of Integrated Traditional Chinese and Western Medicine Tongji HospitalHuazhong University of Science and TechnologyWuhan(430030)China Department of Reproductive Medicine Tongji HospitalHuazhong University of Science and TechnologyWuhan(430030)China

Objective: To evaluate the effect of manual acupuncture on endometrial blood flow parameters by three-dimensional(3D) power Doppler ultrasound in women undergoing in vitro fertilization embryo transfer(IVF-ET). Methods: Seventy patients undergoing IVF-ET were equally randomized into traditional or sham acupuncture treatment group for totally 4 days(from the day of oocyte aspiration to the day of embryo transfer) of treatment by random envelope method at the Reproductive Medicine Center and Outpatient Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology from January 2013 to December 2015. Patients in the traditional acupuncture group accepted traditional acupuncture methods with manual acupuncture, and Zhongji(CV3), Qihai(CV 6), Sanyinjiao(SP6), Taichong(LR 3), Tianshu(ST 25), Guilai(ST 29) and Zusanli(ST 36) were chosen. Patients at the sham acupuncture group accepted shallow acupuncture methods at 4 non-meridian points at each shoulder and upper arm. Outcome measures included endometrial ultrasonic indices such as vascularization index(VI), flow index(FI) and vascularization flow index(VFI), endometrial thickness and volume, subendometrial VI(sVI), subendometrial FI(sFI), subendometrial VFI(sVFI), implantation rate, clinical pregnancy rate, abortion rate, live birth rate and number of live births. Results: Finally, 34 patients in the traditional acupuncture group and 35 in the sham acupuncture group completed this trial. VI, FI and VFI of the traditional acupuncture group were significantly higher than those in the sham acupuncture group(P0.05). Conclusions: Manual acupuncture performed after oocyte aspiration and before transplantation improved the endometr

关键词： acupuncture endometrial blood flow endometrial receptivity in vitro fertilization randomized controlled trial

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Severe male factor in in vitro fertilization:definition,prevalence,and *** update

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Asian Journal of Andrology 2022年第2期24卷 125-134页

作者： Rossella Mazzilli Alberto Vaiarelli Lisa Dovere Danilo Cimadomo NicolòUbaldi Susanna Ferrero Laura Rienzi Francesco Lombardo Andrea Lenzi Herman Tournaye Filippo Maria Ubaldi Clinica Valle Giulia GeneraLife IVF CentersVia G.De NotarisRome 2B 00197Italy Department of Clinical and Molecular Medicine University of Rome“Sapienza”Via di Grottarossa 1035Rome 00189Italy Catholic University of the Sacred Heart Via della Pineta Sacchetti 217Rome 00168Italy Department of Experimental Medicine “Sapienza”University of RomeViale del Policlinico 155Rome 00161Italy Centre for Reproductive Medicine Free University Brussels(UZ Brussel)Brussels 1090Belgium

Infertility affects 10%–15%of couples *** all infertility cases,20%–70%are due to male *** the past,men with severe male factor(SMF)were considered ***,the development of intracytoplasmic sperm injection(ICSI)drastically modified this *** advances in assisted reproductive technology(ART),specifically regarding surgical sperm retrieval procedures,allowed the efficacious treatment of these ***,before undergoing ICSI,male factor infertility requires careful evaluation of clinical and lifestyle behavior together with medical *** speaking,women whose male partner is azoospermic tend to be younger and with a better ovarian *** couples,in fact,are proposed ART earlier in their life,and for this reason,their ovarian response after stimulation is generally ***,in younger couples,azoospermia can be partially compensated by the efficient ovarian response,resulting in an acceptable fertility rate following in vitro fertilization(IVF)***,when azoospermia is associated with a reduced ovarian reserve and/or advanced maternal age,the treatment becomes more challenging,with a consequent reduction in IVF ***,azoospermia seems to impair neither the euploidy rate at the blastocyst stage nor the implantation of euploid *** on the current knowledge,the assessment of male infertility factors should involve:(1)evaluation–to diagnose and quantify seminologic alterations;(2)potentiality–to determine the real possibilities to improve sperm parameters and/or retrieve spermatozoa;(3)time–to consider the available“treatment window”,based on maternal age and ovarian *** review represents an update of the definition,prevalence,causes,and treatment of SMF in a modern ART clinic.

关键词： azoospermia in vitro fertilization infertility intracytoplasmic sperm injection severe male factor sperm

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Modified strict sperm morphology threshold aids in the clinical selection of conventional in vitro fertilization(IVF)or intracytoplasmic sperm injection(ICSI)

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Asian Journal of Andrology 2022年第1期24卷 62-66页

作者： Yong Zhu Feng Zhang Hua Cheng Xiao-Xi Sun Feng Jiang Obstetrics and Gynecology Hospital Fudan UniversityShanghai 200011China Shanghai Ji Ai Genetics and IVF Institute Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China

For infertility treatment,the selection of in vitro fertilization(IVF)or intracytoplasmic sperm injection(ICSI)is decided by multiplying indicators(including fallopian tube factors,semen count,and semen motility),except for sperm *** this study,we conducted a retrospective analysis,from implantation to birth,over a period of 5 years.A total of 1873 couples with primary or secondary fallopian tube factors and an increased defective sperm morphology rate(DSMR)were divided into different groups to receive IVF or ICSI *** comparing the outcomes,we found that the F1 group(DSMR98%,IVF group 3;P98%,ICSI group 3)had higher cleavage rate(Pfertilization rate than the IVF subgroup(P<0.05).Our data suggest that the sperm morphology should also be considered when selecting IVF or ICSI combined with other semen parameters before the first assisted reproductive technologies(ART)cycle,especially for males with severe sperm defects.

关键词： clinical outcome in vitro fertilization intracytoplasmic sperm injection sperm morphology

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Untreated Prior Pulmonary Tuberculosis Adversely Affects Pregnancy Outcomes in Infertile Women Undergoing in vitro fertilization and Embryo Transfer: A Large Retrospective Cohort Study

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Biomedical and Environmental Sciences 2021年第2期34卷 130-138页

作者： GAI Xiao Yan CHI Hong Bin ZENG Lin CAO Wen Li CHEN Li Xue ZHANG Chen LU Ming NING Lan Ding CHANG Chun ZHANG Wei Xia LIU Ping LI Rong SUN Yong Chang QIAO Jie Department of Respiratory and Critical Care Medicine Peking University Third HospitalBeijing 100191China Center for Reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalBeijing 100191China National Clinical Research Center for Obstetrics and Gynecology Beijing 100191China Research Center of Clinical Epidemiology Peking University Third HospitalBeijing 100191China Tuberculosis Department Beijing Geriatric HospitalBeijing 102699China Information Center Peking University Third HospitalBeijing 100191China

Objective Prior pulmonary tuberculosis(PTB) on chest X-ray(CXR) was commonly found in infertile patients receiving examinations before in vitro fertilization and embryo transfer(IVF-ET). It was unclear whether untreated PTB would affect pregnancy outcomes after *** We conducted a retrospective cohort study of 14,254 infertile patients who had received IVFET at Peking University Third Hospital in 2017. Prior PTB was defined as the presence of signs suggestive of old or inactive PTB on CXR, with or without a clinical TB history. Patients who had prior PTB on CXR but had not received a clinical diagnosis and anti-TB therapy were included for analysis. Live birth,clinical pregnancy, and miscarriage rates were compared between the untreated PTB and non-PTB *** The untreated PTB group had significantly lower clinical pregnancy(31.7% vs. 38.1%) and live birth(23.8% vs. 30.6%) rates than the non-PTB group(both P < 0.001). Multivariate analysis revealed that untreated PTB was a risk factor for decreased live birth rate [odds ratio(OR), 0.80;95% confidence interval(CI), 0.66–0.98;P = 0.028] in all patients and for increased miscarriage(OR, 4.19;95% CI,1.69–10.39;P = 0.002) and decreased live birth(OR, 0.45;95% CI, 0.24–0.83;P = 0.011) rates in patients with unexplained *** Untreated PTB was associated with adverse pregnancy outcomes after IVF-ET, especially in patients with unexplained infertility, highlighting the clinical significance of PTB in this specific patient population.

关键词： Tuberculosis Embryo transfer in vitro fertilization Infertility Pregnancy outcome

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Influence of sperm morphology on pregnancy outcome and offspring in in vitro fertilization and intracytoplasmic sperm injection:a matched case-control study

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Asian Journal of Andrology 2021年第4期23卷 421-428页

作者： Wen-Jun Zhou Chuan Huang Su-Hua Jiang Xi-Ren Ji Fei Gong Li-Qing Fan Wen-Bing Zhu Institute of Reproductive and Stem Cell Engineering Basic Medicine CollegeCentral South UniversityChangsha 410008China Reproductive and Genetic Hospital of CITIC-Xiangya Changsha 410008China Clinical Research Center for Reproduction and Genetics in Hunan Province Changsha 410008China

Sperm morphology was once believed as one of the most predictive indicators of pregnancy outcome in assisted reproductive technology(ART).However,the impact of teratozoospermia on in vitro fertilization(IVF)/intracytoplasmic sperm injection(ICSI)outcomes and its offspring remains *** order to evaluate the influence of teratozoospermia on pregnancy outcome and newborn status after IVF and ICSI,a retrospective study was *** was a matched case-control study that included 2202 IVF cycles and 2574 ICSI cycles and was conducted at the Reproductive and Genetic Hospital of CITIC-Xiangya in Changsha,China,from June 2013 to June *** were divided into two groups based on sperm morphology:teratozoospermia and normal sperm *** pregnancy outcome and newborn outcome were *** results indicated that couples with teratozoospermia had a significantly lower optimal embryo rate compared to those with normal sperm morphology in IVF(P=0.007),while there were no statistically significant differences between the two groups in terms of the fertilization rate,cleavage rate,implantation rate,and pregnancy rate(all P>0.05).Additionally,teratozoospermia was associated with lower infant birth weight in multiple births after *** regard to ICSI,there was no significant difference in both pregnancy outcome and newborn outcome between the teratozoospermia and normal groups(both P>0.05).Furthermore,no increase in the risk of birth defects occurred in the teratozoospermia group after IVF/***,we believe that teratozoospermia has limited predictive value for pregnancy outcomes in IVF/ICSI,and has little impact on the resulting offspring if multiple pregnancy is avoided.

关键词： in vitro fertilization intracytoplasmic sperm injection newborn outcome pregnancy outcome teratozoospermia

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Sperm DNA damage has a negative effect on early embryonic development following in vitro fertilization

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Asian Journal of Andrology 2018年第1期20卷 75-79页

作者： Wei-Wei Zheng Ge Song Qi-Ling Wang Shan-Wen Liu Xiao-Li Zhu Shun-Mei Deng An Zhong Yu-Mei Tan Ying Tan Reproductive Medicine Center Family Planning Research Institute of Guangdong Province Guangzhou 510060 China Key Laboratory of Male Reproductive and Genetics Family Planning Research Institute of Guangdong Province Guangzhou 510060 China

Sperm DNA damage is recognized as an important biomarker of male infertility. To investigate this, sperm DNA damage was assessed by the sperm chromatin dispersion （SCD） test in semen and motile spermatozoa harvested by combined density gradient centrifugation （DGC） and swim-up in 161 couples undergoing in vitro fertilization （IVF）. Semen analysis and sperm DNA damage results were compared between couples who did or did not achieve pregnancy. The sperm DNA damage level was significantly different between the two groups （P 〈 0.05） and was negatively correlated with IVF outcomes. Logistic regression analysis confirmed that it was an independent predictor for achieving clinical pregnancy. The effects of different levels of sperm DNA damage on IVF outcomes were also compared. There were significant differences in day 3 embryo quality, blastocyst formation rate, and implantation and pregnancy rates （P 〈 0.05）, but not in the basic fertilization rate between the two groups. Thus, sperm DNA damage as measured by the SCD appears useful for predicting the clinical pregnancy rate following IVF.

关键词： DNA damage in vitro fertilization pregnancy outcome sperm

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Intracytoplasmic sperm injection in cases with a history of in vitro fertilization failure

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Asian Journal of Andrology 2003年第1期5卷 69-72页

作者： Zhi-Ling LI, Hong LIN, Xin-Neng ZHANG, Wan-Fen XIAO Reproduction Center, First Affiliated Hospital, Medical College, Shantou University, Shantou 515041, China Reproduction Center First Affiliated Hospital Medical College Shantou University Shantou 515041 China. lizhilin@***

Aim: To evaluate the effect of intracytoplasmic sperm injection (ICSI) in the management of cases with a history of conventional in vitro fertilization (IVF) failure. Methods: Two groups of patients, 19 with normal semen parameters and a history of IVF failure (metaphase Ⅱ oocytes: 0~30 %) and 28 with severe male factor infertility received ICSI technology during the same period. Ovarian stimulation was achieved by conventional procedure. Transvaginal ultrasound-guided oocyte collection was done 35~37 h after human chorionic gonadotrophin (hCG) injection. Only metaphase Ⅱ oocytes were selected for microinjection. Results: fertilization was achieved with ICSI in all the patients. The fertilization rate (75.6 %±21.1 % vs. 73.9 %±19.2 %), cleavage rate (85.1 %±19.3 % vs. 82.7 %±22.1 %), clinical pregnancy rate per embryo transfer cycle (31.6 % vs. 28.6 %) and implantation rate per embryo (15.3 % vs. 14.4 %) did not differ significantly between the two groups. Conclusion: ICSI is a valuable method for couples with a history of IVF failure. These patients may have a similar ICSI result as in severe male infertility.

关键词： intracytoplasmic sperm injection in vitro fertilization male factor infertility fertilization failure

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in vitro fertilization of Angiosperms-10-Year Effort in China

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Acta Botanica Sinica 2002年第9期44卷 1011-1021页

作者：孙蒙祥杨弘远武汉大学植物发育生物学教育部重点实验室武汉430072

This review gives a brief retrospect to the development on in vitro fertilization (IVF) of angiosperms in China. During the last decade Chinese scientists put great enthusiasm and efforts on IVF system construction and built up notable contributions to the flourish of this field. Keeping pace with international development and participating international cooperation in the field of IVF, Chinese scientists have now focused on the investigation of basic mechanism relevant to possible gamete interaction, egg cell activation and early embryogenesis by IVF. In vitro manipulation techniques are combined with cytological and molecular biological approaches to unveil the double fertilization mysteries.

关键词： gamete in vitro fertilization angiosperm

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