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Effects of inclusion of purple prairie clover (Dalea purpurea Vent.)with native coolseason grasses on in vitro fermentation and in situ digestibility of mixed forages

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Journal of Animal Science and Biotechnology 2020年第2期11卷 563-576页

作者： Kai Peng Gemma L.Gresham Tim A.McAllister Zhongjun Xu Alan Iwaasa Mike Schellenberg Alex V.Chaves Yuxi Wang Key Laboratory of Animal Nutrition and Feed Science(South China)of Ministry of Agriculture Guangdong Key Laboratory of Animal Breeding and NutritionInstitute of Animal ScienceGuangdong Academy of Agricultural ScienceGuangzhou 510640People’s Republic of China Agriculture and Agri-Food Canada Lethbridge Research and Development CentreLethbridgeAB T1J 4B1Canada School of Life and Environmental Sciences The University of SydneySydneyNSW 2006Australia Agriculture and Agri-Food Canada Swift Current Research and Development CentreSwift CurrentSK S9H 3X2Canada

Background:Incorporation of legume species into native North American pastures is considered an effective method to increase native pasture productivity and improve the nutritive value of *** study evaluated the effects of inclusion of purple prairie clover(PPC,Dalea purpurea Vent.),a native legume forage,with native coolseason grasses on the in vitro fermentation and in situ digestibility of mixed ***:Whole plant PPC and mixtures of cool-season grasses were harvested when the PPC reached the vegetative(VEG),full flower(FL)and seedpod(SP)stages,and were combined in ratios(DM basis)of 0:100,25:75,50:50,75:25 and 100:0 at each *** vitro ruminal incubations using these mixtures were conducted for 48 h to determine gas production(GP),in vitro DM disappearance(IVDMD),total volatile fatty acids(VFA)and ammonia-N *** of forages harvested when the PPC reached the FL stage and 50:50 mixture of forages harvested at VEG,FL and SP stages were incubated in the rumen of three heifers for 0,2,6,12,24,48,72 and 96 h to determine in situ degradabilities of DM,neutral detergent fibre(aNDF)and crude protein(CP).Results:Contents of aNDF and ADF increased(PVEG>SP(P<0.05).Regardless of PPC proportions in the mixture,GP decreased(P<0.05)with increasing PPC *** PPC proportions linearly increased(P<0.001)GP,IVDMD and total VFA at VEG,but linearly decreased(P<0.001)them at *** of PPC maturity,ammonia-N production linearly increased(P<0.01)with increasing proportions of PPC and the concentration was higher(P<0.05)at VEG than at FL and SP *** proportion of PPC at either maturity linearly increased(P<0.001)molar percentage of acetate(A)and branched-chain VFA,but linearly decreased(P<0.001)molar percentage of propionate(P),resulting in a linearly increase(P<0.001)in the A:P *** FL PP

关键词： Condensed tannins Cool-season grass In vitro fermentation in situ digestibility Purple prairie clover

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