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Application of in situ cultivation in marine microbial resource mining

Marine Life Science & Technology 2021年第2期3卷 148-161页

作者： Dawoon Jung Liwei Liu Shan He Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center College of Food and Pharmaceutical SciencesNingbo UniversityNingbo315832China

Microbial communities in marine habitats are regarded as underexplored reservoirs for discovering new natural products with potential ***,only a few microbes in nature can be cultivated in the *** has led to the development of a variety of isolation and cultivation methods,and in situ cultivation is one of the most *** in situ cultivation methods,with the same basic principle,have been applied to a variety of environmental *** with conventional approaches,these new methods are able to cultivate previously uncultured and phylogenetically novel microbes,many with biotechnological *** review introduces the various in situ cultivation methods for the isolation of previously uncultured microbial species and their potential for marine microbial resource ***,studies that investigated the key and previously unidentified mechanisms of growing uncultivated microorganisms by in situ cultivation,which will shed light on the understanding of microbial uncultivability,were also reviewed.

关键词： Uncultured microorganism Marine microbial resource in situ cultivation Microbial diversity

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FACS-iChip:a high-efficiency iChip system for microbial‘dark matter’mining

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Marine Life Science & Technology 2021年第2期3卷 162-168页

作者： Haoze Liu Ran Xue Yiling Wang Erinne Stirling Shudi Ye Jianming Xu Bin Ma Institute of Soil and Water Resources and Environmental Science College of Environmental and Resource SciencesZhejiang UniversityHangzhou310058China Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment Zhejiang UniversityHangzhou310058China

The isolation chip method(iChip)provides a novel approach for culturing previously uncultivable microorganisms;this method is currently limited by the user being unable to ensure single-cell loading within individual *** address this limitation,we integrated flow cytometry-based fluorescence-activated cell sorting with a modified iChip(FACS-iChip)to effectively mine microbial dark matter in *** method was used for paddy soils with the aim of mining uncultivable microorganisms and making preliminary comparisons between the cultured microorganisms and the bulk soil via 16S rRNA gene *** showed that the FACS-iChip achieved a culture recovery rate of almost 40%and a culture retrieval rate of 25%.Although nearly 500 strains were cultured from 19 genera with 8 FACS-iChip plates,only six genera could be identified via 16S rRNA gene *** result suggests that the FACS-iChip is capable of detecting strains in the currently dead spaces of PCR-based sequencing ***,therefore,conclude that the FACS-iChip system provides a highly efficient and readily available approach for microbial‘dark matter’mining.

关键词： FACS-iChip Microbial‘dark matter’ in situ cultivation Single cell sorting

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