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Surface enhanced Raman spectroscopy signals of mixed pesticides and their identification

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Chinese Chemical Letters 2011年第12期22卷 1477-1484页

作者： Hui Rong Tang Qing Qing Li Yu Lan Ren Jin Pei Geng Peng Cao Tao Sui Xuan Wang Yi Ping Du Shanghai Key Laboratory of Functional Materials Chemistry and Research Centre of Analysis and Test East China University of Science and TechnologyShanghai 200237China Mudanjiang Normal University Mudanjiang 157012China Yantai Entry-Exit Inspection and Quarantine Bureau Yantai 264000 China

A rapid and sensitive method to identify and analyze mixed pesticides of tricyclazole,paraquat and flusilazole by surface-enhanced Raman scattering has been *** samples can be detected by SERS in low concentrations of 0.01 mg/L, 0.1 mg/L and 2.85 mg/L for individual tricyclazole,paraquat and flusilazole *** mixing the three pesticides in the low concentrations,their characteristic peaks can still be identified from the SERS spectrum of the mixture.

关键词： Surface-enhanced Raman scattering(SERS) Pesticide Mixed pesticides identification

Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing

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Synthetic and Systems Biotechnology 2023年第4期8卷 708-715页

作者： Jinyu Cui Rongze Chen Huili Sun Yingyi Xue Zhidian Diao Jingyun Song Xiaohang Wang Jia Zhang Chen Wang Bo Ma Jian Xu Guodong Luan Xuefeng Lu Key Laboratory of Biofuels Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesNo.189 Songling RoadQingdao266101China Shandong Energy Institute Qingdao266101China Qingdao New Energy Shandong Laboratory Qingdao266101China College of Life Science University of Chinese Academy of SciencesBeijingChina Dalian National Laboratory for Clean Energy Dalian116023China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdaoChina

By directly converting solar energy and carbon dioxide into biobased products,cyanobacteria are promising chassis for photosynthetic *** make cyanobacterial photosynthetic biosynthesis technology economically feasible on industrial scales,exploring and engineering cyanobacterial chassis and cell factories with fast growth rates and carbon fixation activities facing environmental stresses are of great *** simplify and accelerate the screening for fast-growing cyanobacteria strains,a method called Individual Cyanobacteria Vitality Tests and Screening(iCyanVS)was *** show that the ^(13)C incorporation ratio of carotenoids can be used to measure differences in cell growth and carbon fixation rates in individual cyanobacterial cells of distinct genotypes that differ in growth rates in bulk cultivations,thus greatly accelerating the process screening for fastest-growing *** feasibility of this approach is further demonstrated by phenotypically and then genotypically identifying individual cyanobacterial cells with higher salt tolerance from an artificial mutant library via Raman-activated gravity-driven encapsulation and ***,this method should find broad applications in growth rate or carbon intake rate based screening of cyanobacteria and other photosynthetic cell factories.

关键词： Cyanobacteria Single-cell Raman microspectroscopy Stable isotope probing Carotenoids identification

Isolation and identification of Rhodosporidium diobovatum DS-0205 from deep-sea sediment of eastern Pacific Ocean

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Journal of Central South University 2009年第6期16卷 942-947页

作者：曾乐平黄菊芳邱冠周初凤友陈旦童建斌罗学港 Xiangya School of Medicine Central South University Key Laboratory of Submarine Geosciences of State Oceanic Administration School of Resources Processing and Bioengineering Central South University

A facultative heterotrophic strain （DS-0205） was isolated from a deep-sea sediment sample collected at a depth of 5.2 km in the eastern Pacific Ocean, China. Strain DS-0205 is motile helmet-like single cell or pairs, forming hemisphere with the center sunken of variable size. It has a widespread carbon source and nitrogen source, including agarose, citric acid, salicin, D-glucitol nitrate, sodium nitrite and cthylamine, tt can grow in the following environment： temperature 4-37℃, pH 2.0-12.0, tolerance to NaCl≤15%. Two phylogenetic trees, one based on the ITS and 5.8S rRNA sequences and the other based on the 18S rRNA sequences, unite strain DS-0205（=JCM 0205） to the type strain of Rhodosporidium diobovatum JCM 3787 through a considerable evolutionary distance. These results suggest that strain DS-0205 is a new strain of the Rhodosporidium diobovatum.

关键词： deep-sea sediment ITS and 5.8S rRNA sequences 18S rRNA isolation identification

Molecular identification based on ITS sequences for Kappaphycus and Eucheuma cultivated in China

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Chinese Journal of Oceanology and Limnology 2011年第6期29卷 1287-1296页

作者：赵素芬何培民 College of Fisheries and Life Science Shanghai Ocean University Fisheries College Guangdong Ocean University

The systematic classification of the Eucheumatoideae is difficult because of their variable morphology and interpretation of reproductive structures. Kappaphycus and Eucheuma specimens cultivated on the Hainan and Fujian coast of China were introduced from Vietnam, the Philippines and Indonesia. Combined with morphological characteristics, all Kappaphycus and Eucheuma cultivated strains were identified by internal transcribed spacer (ITS) sequences. The phylogenetic tree was constructed using neighbor-joining and maximum likelihood methods. The results indicate that different ITS sequence lengths occurred in the different genera and species. An obvious difference in morphology could be found in the protuberance shape between Kappaphycus and Eucheuma. The protuberance in Eucheuma was thorn-like and in Kappaphycus was wartlike or papillate. Their ITS sequence lengths differed significantly in nucleotide variation rates up to 58.55%-63.90%. All nucleotide variations occurred in the ITS1 and ITS2 regions except for five nucleotide transversions in the 5.8S rDNA region. In addition, the difference was at the branches among congeneric species. Kappaphycus sp. had branches with small buds, while K. alvarezii did not have such a feature. The nucleotide variation rates varied from 7.02% to 7.48% among species; within the same species of the clades it was identification of interspecies and intraspecies phylogenetic relationships and might provide a clue for molecular identification of algal Eucheumatoideae.

关键词： Eucheuma identification Kappaphycus ITS Rhodophyta

Robust identification for rational fractional transfer functions

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Science China Mathematics 1997年第4期40卷 366-374页

作者：王书宁 Institute of Systems Engineering Huazhong University of Science and Technology Wuhan 430074 China

An algorithm is proposed for robust identification of a rational fractional transfer function with a fixed degree under the framework of worst-case/deterministic robust identification. The convergence of the algorithm... 详细信息

关键词： robust control oriented system identification worst-case/deterministic identification parameter estimation rational fractional model.

Molecular identification and population dynamics of two species of Pemphigus （Homoptera： Pemphidae） on cabbage

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Insect Science 2009年第2期16卷 115-124页

作者： Naiqi Chen Tong-Xian Liu Mamoudou Setamou J. Victor French Eliezer S. Louzada Department of Entomology Texas AgriLife Research Texas A&M University System Weslaco Citrus Center Texas A&M University- Kingsville Citrus Center Weslaco TX 78596 USA

The poplar petiole gall aphid, Pemphiguspopulitransversus Riley, has been one of the major pests on cruciferous vegetable in the Rio Grande Valley （LRGV） of Texas since the late 1940s. It normally migrates from poplar trees to cruciferous vegetables in the fall, and migrates back to the trees in early spring of the coming year. Some root-feeding aphids were found on cruciferous vegetables in late spring and early summer in 1998 and the following years. Those aphids have been identified as Pemphigus obesinymphae Moran. This discovery completely changed the current knowledge about the root-feeding aphids on cruciferous vegetables in the LRGV. Due to their small size, morphological and feeding similarities between P. populitransversus and P. obesinymphae, their identification and distinction are difficult. In this study, random amplification of polymorphic DNA （RAPD） and amplified fragment length polymorphism （AFLP） were used to distinguish these two species over a period of time when the two species occurred together, or separately, in cabbage fields. The two species occurred on cabbage at different times of the year, and overlapped from October to June. From May to October, both species migrated to their primary hosts. The apterous aphids found on cabbage in winter contained mainly P. obesinymphae, whereas in early spring more apterous P. populitransversus were recovered. The root-feeding aphids would feed on cabbage plants as long as this host was available even during the hot, dry summer in the LRGV, although their populations were generally low. Both RAPD and AFLP techniques were efficient in discriminating the two species that showed obviously genetic variability. These molecular techniques confirmed the existence of the two aphid species in apterous samples collected from the soil in cabbage fields in the LRGV, and the results performed by RAPD were confirmed by AFLP. Furthermore, the results suggest that RAPD technique was a better choice despite its reproducib

关键词： AFLP cabbage identification RAPD root aphid

Characteristics of Heavy Minerals and Quantitative Provenance identification of Sediments from the Muddy Area Outside the Oujiang Estuary Since 5.8 kyr

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Journal of Ocean University of China 2018年第6期17卷 1325-1335页

作者： SONG Hongying LIU Jinqing YIN Ping ZHANG Yong CHEN Xiaoying Center for Isotope Geochemistry and Geochronology Qingdao National Laboratory for Marine Science and Technology Key Laboratory of Marine Environmental Geology Ministry of Natural Resources Qingdao Institute of Marine Geology Laboratory for Marine Geology Qingdao National Laboratory for Marine Science and Technology College of Earth Science and Engineering Shandong University of Science and Technology

The heavy mineral compositions of the sediments in core D04 with a length of 20.2 m from the muddy area outside the Oujiang Estuary along the coast of Zhejiang-Fujian Provinces have been analyzed. Core D04 can be divided into three units: DU1(0–7.4 m), DU2(7.4–11.4 m) and DU3(11.4–20.2 m). The results showed that the heavy minerals are authigenic pyrite(65.6%)– hornblende(16.0%) – epidote(4.6%) assemblages. The core sediments are mainly composed of clayey silt, which belongs to the clinoform deposit formed since 5.8 kyr, indicating a weak hydrodynamic environment. The deposition rates changed in the trend of high-low-high upward. Quantitative analysis shows that the core sediments were mainly from the suspended sediments of the Changjiang(Yangtze) River, accounting for 79.2%, with only 10.0% from Oujiang, and 10.8% from other provenance. The Oujiang-derived sediments were gradually increased from the bottom unit DU3(3.1%) to the top unit DU1(17.8%), but the Changjiang-derived sediments were gradually decreased. The source changes are closely related to the development of Yangtze River Delta and Wenzhou Bay, climate changes and human activities. During the period of 2.1–3.7 kyr, the sediment supply was greatly reduced due to the poor reclamation capacity of the river basins, resulting in a low deposition rate. Since 2 kyr, the enhancement of human activities in the Yangtze River Basin and the Oujiang River Basin led to more soils to be eroded and transported to the study area. Due to the short distance of the site of core D04 from the Oujiang River mouth, the study area received more Oujiang-derived sediments when upper unit(DU1) deposited than the lower units(DU2 and DU3).

关键词： mud deposit Oujiang Estuary mid-Holocene heavy mineral quantitative identification

Development of fault section identification technique for low voltage DC distribution systems by using capacitive discharge current

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Journal of Modern Power Systems and Clean Energy 2018年第3期6卷 509-520页

作者： Chul-Ho NOH Chul-Hwan KIM Gi-Hyeon GWON Yun-Sik OH College of Information and Communication Engineering Sungkyunkwan University

The increasing importance of energy efficiency has led to several studies related to the construction of a reliable low voltage DC(LVDC) distribution ***, studies on a protection scheme that considers the fault characteristics of an LVDC distribution system are essential to improve system reliability. When compared to a conventional distribution system, the most distinct feature of an LVDC distribution system is the existence of a capacitive discharge current from converters under a fault condition that results in the prompt operation of protection devices. Therefore, this study involves proposing a precise and rapid technique to identify the fault section in an LVDC distribution system. The technique involves two stages, namely: an analysis stage to analyze the capacitive discharge current and a decision stage to identify the fault section based on the fault type. A detailed discussion of each step is presented and its feasibility is verified based on the results of simulations with an ElectroMagnetic Transients Program and MATLAB~?.

关键词： Capacitive discharge current Fault section identification Fault type identification Low voltage DC distribution system

A new method for nondestructive damage identification of lattice materials

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Science China(Technological Sciences) 2009年第5期52卷 1293-1300页

作者： FANG Hui WANG TieJun MOE Key Laboratory for Strength and Vibration Department of Engineering MechanicsXi’an Jiaotong UniversityXi’an 710049China

This paper proposes vibration-based damage identification method,termed as substructure potential energy(SPE) method,which is capable of accurately estimating the damage magnitudes of multiple *** other existing damage severity estimation methods require the information of several unabridged modes of the structure,the new method utilizes only a few lower mode shapes of sub-structures measured from the damaged *** performance of the proposed method is compared with one existing damage detection method,using a set of numerical simulations on a lattice material beam based on synthetic data generated from finite element models.

关键词： lattice material damage identification strain energy