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Genetic variation of the genus Kengyilia by issr markers

生物学前沿:英文版 2008年第2期3卷 170-175页

作者： Li ZHANG Yonghong ZHOU Chunbang DING Ruiwu YANG Shigui LIU Triticeae Research Institute Sichuan Agricultural UniversityDujiangyan 611830China National Laboratory of Grassland Biological Control Sichuan UniversityChengdu 610064China

We investigated the genetic variation within 32 accessions distributed to 14 species and one variety by using issr(inter-simple sequence repeat)*** results showed that genetic variation was relatively higher among the accessions.A total of 593 bands were amplified by 12 issr primers,of which 535 bands(90.2%)were *** to 80 polymorphic bands were amplified from each prime,with an average of 44.6 *** interspecies GS(genetic similarity)value ranged from 0.430 to 0.866,and the average was *** analysis showed that all accessions could be classified into 4 groups by issr *** different accessions in a species were clustered together,but they had genetic variation in molecular *** was obvious interspecies genetic *** with similar morphological characteristics and from the same areas or neighboring geographical regions were clustered together and had close *** markers are useful in analyzing interspecies variation in Kengyilia.

关键词： Kengyilia issr markers genetic variation

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Natural hybridization and introgression in sympatric Ligularia species(Asteraceae,Senecioneae)

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Journal of Systematics and Evolution 2011年第5期49卷 438-448页

作者： Chiaki KURODA Department of Chemistry Rikkyo University

The difficulty in clarifying species of genus Ligularia *** been attributed to rapid and continuous allopatric speciation in small and isolated populations,combined with interspecific diploid hybridization in the Qinghai-Tibetan Plateau and adjacent ***,no concrete example has been reported to prove this *** studied a natural mixed population of six species of Ligularia in which some individuals were morphologically intermediate between *** and *** on DNA sequences(trnC-F,trnL-rpL32,trnQ5 rps16,trnK-rps16,and internal transcribed spacer) and inter-simple sequence repeat data,we concluded that putative hybrids are primarily products of hybridization between *** and *** other four species or additional,unknown species may also be involved in *** hybridization is bidirectional but *** individuals were mostly the first generation,but F2 and later-generation hybrids were also ***,the backcrossed individuals detected indicate that natural gene flow occurs among at least three Ligularia *** may become stabilized to form new species or may function as intermediates in evolutionary diversification.

关键词： chloroplast fragments issr markers Ligularia natural hybridization nuclear ribosomal ITS.

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Low genetic diversity and high genetic differentiation in the critically endangered Omphalogramma souliei (Primulaceae):implications for its conservation

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Journal of Systematics and Evolution 2009年第2期47卷 103-109页

作者： Yuan HUANG Chang-Qin ZHANG De-Zhu LI(Key Laboratory of Biodiversity and Biogeography,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China) Key Laboratory of Biodiversity and Biogeography Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China

Omphalogramma souliei *** an endangered perennial herb only distributed in alpine areas of SW *** markers were applied to determine the genetic variation and genetic structure of 60 individuals of three populations of *** in NW Yunnan,*** genetic diversity at the species level is low with P= 42.5%(percentage of polymorphic bands) and Hsp=0.1762(total genetic diversity).However,a high level of genetic differentiation among populations was detected based on different measures(Nei’s genetic diversity analysis:Gst=0.6038;AMOVA analysis:Fst=0.6797).Low level of genetic diversity within populations and significant genetic differentiation among populations might be due to the mixed mating system in which xenog-amy predominated and autogamy played an assistant role in *** genetic drift due to small population size and limited current gene flow also resulted in significant genetic *** assessment of genetic variation and differentiation of the endangered species provides important information for conservation on a genetic *** strategies for this rare endemic species are proposed.

关键词： genetic differentiation genetic diversity issr markers mating system Omphalogramma souliei

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Diversity among Isolates of the Tangerine Pathotype of Alternaria alternata

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American Journal of Plant Sciences 2018年第2期9卷 196-215页

作者： Andréa Celina Ferreira Demartelaere Luciana Cordeiro do Nascimento Cristina O.Marinho Marciano Costa Nunes Patrícia Clemente Abraao Rommel S.S.Gomes Monica Danielly de Mello Oliveira Larissa Almeida Mirelly M.Porcino Caroline Vargas e Silva Wilza C.O.Souza Programa de Pós-Graduacao em Agronomia Departamento de Fitotecnia e Ciencias AmbientaisUniversidade Federal da ParaíbaAreiaBrazil

The tangerine pathotype of Alternaria alternata is the aetiological agent of Alternaria brown spot on tangerines. In the state of Paraíba, Brazil, its occurrence on “Dancy” tangerine trees is associated with genetic aspects as well as the influence of environmental conditions on reproduction and dissemination within and between populations. The aim of the present study was to evaluate the diversity of isolates of this pathogen using morphophysiological and molecular markers. For the analysis of mycelial growth and sporulation, 30 isolates from different locations were examined at 24-hour intervals until the seventh day, when the spores were quantified. The 30 isolates were characterised based on molecular markers (issr) and genetic similarity (Jaccard index). A factor arrangement was used: 30 isolates, four media (ODA, PDA, LEA and V8), three light regimes (continuous dark, alternating light and continuous light) and three temperatures (15°C, 25°C and 35°C), with 12 repetitions. Groups 1, 2 and 3 presented low genetic variability. Group 4 showed high genetic variability of the isolates obtained from the Massaranduba (state of Paraíba-Brazil) producing region and higher mycelial growth and sporulation of A. alternata. The continuous light regime and the temperature 25°C in PDA and V8 media were the ideal conditions for the mycelial growth and sporulation, respectively, of the isolates of A. alternata.

关键词： Citrus tangerine Alternaria Brown Spot Fungal Variability Environment Factors issr markers

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Interpopulation Congruence in Chinese Primula ovalifolia Revealed by Chemical and Molecular markers Using Essential Oils and issrs

Interpopulation Congruence in Chinese Primula ovalifolia Rev...

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第七届全国系统与进化植物学青年学术研讨会

作者： NAN Peng, PENG Shao-Lin, SHI Su-Hua, REN Hai, YANG Ji ZHONG Yang (1. South China Institute of Botany, Academia Sinica, Guangzhou 510650, China 2. Wuhan Institute of Botany, Academia Sinica, Wuhan 430074, China 3. School of Life Sciences, Zhongshan University, Guangzhou 510275, China 4. College of Life Sciences, Peking University, Beijing 100871, China 5. Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China.)

The chemical composition of the essential oils of five natural populations of P. ovalifolia from central and southwest China and their interpopulation variability were first analyzed by using GC-MS. Twenty-two essential oil compounds were obtained, in which eighteen ones were identified and characterized representing 95% - 96% of the oil composition. Three main chemotypes, i.e., the methyl-acetyl-hydroquinone-rich, hydroquinone-rich, and acetyl-hydroquinone-rich chemotypes,

关键词： Primula ovalifolia Essential Oil issr markers

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