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Human hematopoietic cells express two forms of thecytokine receptor common γ-chain (γc)

Cell Research 1997年第2期7卷 195-205页

作者： SHI YU FANG MARY HilL ANTON NOVAK ZHIQING CHEN RUO XIANG WANG CHOONGCHIN LIEW GORDON B. MilLS (Oncology Research, The Toronto Hospital, 200 ElizabcthSt., Toronto, Ontario, Canada MSG 2C4Department of Immunology, Holland Laboratoryof American Red Cr Oncology Research The Toronto Hospital Toronto Canada Department of Immunology Holland Laboratory of American Red Cross Rockville USA Department of Genetics Research Institute The Hospital for Sick Children Toronto Canada Department of Clinical Biochemistry University of Toronto Toronto Canada Department of Molecular Oncology MD Anderson Cancer Center University of Texas Houston

Recent studies have revealed that the γ-chain of theil-2 receptor is shared by the receptors for il-4, il7, il-9, il-13, and il-15, and it is therefore also referred toas the common γ-chain (γc). Mutations of γc result inX-linked severe combined immunodeficiency syndrome inhumans, indicating that rye is essential for normal development and function of the immune system. We demonstratethat human hematopoietic cells express two γc transcriptsdiffering in their carboxyl terminal coding region. Onetranscript is the previously reported sequence (γc-long),whereas the newly identified sequence exhibits a deletion of72 nucleotides close to the 3'-end of the open reading frame(γc-short). This alteration predicts a loss of 24 amino acidsincluding a conserved tyrosine residue which is shared byseveral members of the cytokine receptor family. Thepresence of these two distinct forms of rye transcripts wasdemonstrated by sequencing of reversely transcribed andpolymerase chain reaction (RT-PCR) amplified mRNA, restriction digestion of the RT-PCR products, RNAse protection, and Northern blotting from human cell lines andhuman peripheral blood lymphocytes. Furthermore, thetwo variants were present in peripheral blood lymphocytesfrom both female and male donors, which rules out allelicvariants since rye is a single copy gene located on the Xchromosome. A truncation mutant at a site near the observed changes in γc-short has been reported by othersto alter biochemical events activated by cytokines. Thiscombined with the loss of a potential SH2 "docking" sitein γc-short suggests that γc-long and γc-short may link todifferent signaling pathways and may play an importantrole in determining the cellular response to il-2, il-4, il-7, il-9, il-13, il-15.

关键词： il-2 receptor γ-chain cytokine receptor common γ-chain il-2 receptor cytokine receptors RNA splicing

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Analysis of il-2-like factor in lymphocyte culture supernatant of olive flounder, Paralichthys oliveaceus

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Chinese Journal of Oceanology and Limnology 2005年第1期23卷 60-64页

作者：乌日琴张培军李军徐永立 Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Institute of Postgraduate Chinese Academy of Sciences Beijing 100039 China Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China

To study immune mechanism of fish lymphocyte we performed a proliferation assay and ELISA using monoclonal antibody against human il-2. The result showed that an interleukin-2 （il-2）-like factor was detected in the supernatant of plant haemoglutinin （PHA）-stimulated lymphocyte culture from peripheral blood,spleen and head kidney of olive flounder, Paralichthys olivaceus. The quantities of il-2-1ike factor in the supernatant from different lymphoid tissues were quite different. The il-2 like factor in the supernatant from cultured head kidney lymphocytes was much higher than those of peripheral blood lymphocytes and spleen lymphocytes （P〈0.01）. The il-2 activity was found in either mouse thymocyte proliferation assay or flounder head kidney lymphocyte proliferation assay and shown to have obvious enhancing effect on proliferation of the above two types of cell. The recombinant human il-2 （rhil-2） was able to stimulate flounder thymocyte proliferation and used to detect the il-2 receptor （il-2R） on the surface of flounder lymphocyte. The cross-reaction between the lymphocytes of flounder peripheral blood and CD25（il-2R） was detected with flow cytometry and shown that the percentage of CD25-positive cell in peripheral blood was 7.74±0.67%.

关键词： Interleukin-2 il-2 receptor olive flounder lymphocyte

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