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Human ifn-k Inhibited Respiratory RNA Virus Replication Dependent on Cell-to-Cell Interaction in the Early Phase

Infectious Diseases & Immunity 2022年第2期2卷 65-73页

作者： Weihui Fu Peng Sun Jun Fan Longfei Ding Songhua Yuan Guanxing Zhai Miaomiao Zhang Chenli Qiu Shuye Zhang Xiaoyan Zhang Jianqing Xu Shanghai Public Health Clinical Center&Institutes of Biomedical Sciences Fudan UniversityShanghai 201508China

Background:Interferon kappa(ifn-k)is a type I interferon(ifn-I)that inhibits virus replication by evoking interferon-stimulated genes(ISGs).However,as an evolutionarily ancient interferon,ifn-k may function differently from the later emerged interferon-a and ***:Conventional molecular biology methods were used to determine the localization of ifn-k and its structure and *** addition,we employed RT-PCR,western blot,and RNA-Seq technologies to characterize the ISGs expression profile and antiviral activities exerted by ifn-k or ***:Human ifn-k exists in two forms upon ectopic expression,one located on the cell membrane and the other secreted outside the *** membrane-anchored ifn-k showed the ability to induce ISGs and curtail RNA virus replication,whereas the secreted ifn-k failed to do *** analyses indicated that 1-27aa at the N-terminus was the signal peptide,and 28-37aa was predicted as the transmembrane ***,our data demonstrated that both of them were not associated with membrane localization of ifn-k;the former influenced the expression and secretion of ifn-k,and the latter had an impact on the induction of *** addition,prokaryotic purified soluble mature human ifn-k was also capable of inducing ISGs and inhibiting RNA virus ***,human ifn-k induced a faster ISG response but with a lower intensity and a shorter half-life than the response of *** contrast,ifn-a2 started to function later but was stronger and more durable than ***:Human ifn-k-induced ISG response and inhibited respiratory RNA virus replication dependent on cell-to-cell *** addition,compared with ifn-a2,ifn-k exerted effects more rapidly in the early phase,with less intensity and a shorter ***,ifn-k may constitute the first line of ifn-I against respiratory virus infections.

关键词： Interferon type I ifn-k Influenza Mechanism SARS-CoV-2

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