检索结果-南通市图书馆

Identification of new type I interferon- stimulated genes and investigation of their involvement in ifn-β activation

引用

Protein & Cell 2018年第9期9卷 799-807页

作者： Xiaolin Zhang Wei Yang Xinlu Wang Xuyuan Zhang Huabin Tian Hongyu Deng Liguo Zhang Guangxia Gao CAS Key Laboratory of Infection and Immunity CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China

Virus infection induces the production of type I interferons （ifns）. ifns bind to their heterodimeric receptors to initiate downstream cascade of signaling, leading to the up-regulation of interferon-stimulated genes （ISGs）. ISGs play very important roles in innate immunity through a variety of mechanisms. Although hundreds of ISGs have been identified, it is commonly recognized that more ISGs await to be discovered. The aim of this study was to identify new ISGs and to probe their roles in regulating virus-induced type I ifn production. We used consensus interferon （Con-ifn）, an artificial alpha ifn that was shown to be more potent than naturally existing type I ifn, to treat three human immune cell lines, CEM, U937 and Daudi cells. Microarray analysis was employed to identify those genes whose expres- sions were up-regulated. Six hundred and seventeen genes were up-regulated more than 3-fold. Out of these 617 genes, 138 were not previously reported as ISGs and thus were further pursued. Validation of these 138 genes using quantitative reverse transcription PCR （qRT-PCR） confirmed 91 genes. We screened 89 genes for those involved in Sendal virus （SeV）-induced ifn-13 promoter activation, and PIM1 was identified as one whose expression inhibited SeV-mediated ifn-β activation. We provide evidence indicating that PIM1 specifically inhibits RIG-I- and MDA5-mediated ifn-β signaling. Our results expand the ISG library and iden- tify PIM1 as an ISG that participates in the regulation of virus-induced type I interferon production.

关键词： interferon-stimulated genes ifn-β signaling PIM1 RIG-I MDA5

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：