检索结果-南通市图书馆

Angiogenic Effect of Intercellular Adhesion Molecule-1

Journal of Huazhong University of Science and Technology(Medical Sciences) 2007年第1期27卷 9-12页

作者：邓成国张端莲陕声国伍静文杨虹余瑛 Department of Histology & Embryology Yunyang Medical College Department of Plastic Surgery Renmin Hospital Wuhan University Department of Anthropotomy and Histology & Embryology School of Medicine

In order to investigate the angiogenic effect of intercellular adhesion molecule-1 （icam-1）, two parts of experiment were performed. Chick embryo chorioallantoic membrane （CAM） assay was used for in vivo angiogenic research. The chick embryos were divided into 4 groups： icam-1 group （divided into 3 subgroups, Ⅰ, Ⅱ and Ⅲ） for screening the angiogenic effect of icam-1 by adding different concentrations of icam-1 （0.1, 0.2 and 0.3 μg/μL） 5 μL into the chick embryo CAMs on the day 10 after incubation for every subgroup; Anti-icam-1 group A （divided into 2 subgroups, Ⅰ and Ⅱ） by adding different concentrations of Anti-icam-1 （1：100, 1：50） 5 μL into the chick embryo CAMs on the day 10 after incubation for every subgroup to evaluate the effect of icam-1 on the survival of microvessels through observing whether Anti-icam-1 could induce involution of the microvessels on CAMs; Anti-icam-1 group B （divided into 2 subgroups, Ⅰ and Ⅱ ） by adding different concentrations of Anti-icam-1 （1：100, 1：50） 5 μL into the chick embryo CAMs on the day 6 after incubation for every subgroup to evaluate whether icam-1 involved in embryonic angiogenesis through observing the growth of microvessels on CAMs; Control group： icam-1 or Anti-icam-1 was substituted by PBS 5 μL on the day 10 or day 6 after incubation. Three days later, the CAMs were photographed in vivo, excised, sectioned and the number of microvessels was counted. In icam-1 group, there was increased number of microvessels arranged radially with ＂spoked-wheel＂ pattern around the gelatin sponges. The new microvessels growing perpendicularly to gelatin sponges were observed. The number of the microvessels growing in the CAM mesenchymes around the sponges in 3 subgroups was higher than that in control group （P〈0.01）, however, there was no significant difference among the 3 subgroups （P〉0.05）. In anti-icam-1 group A, the radially arranged microvessels were very unclear around the sponges contrast to that of icam-1 group. Few new microvessels were detected in the center of the sponges

关键词： icam- 1 angiogenesis immunohistochemistry chick embryo chorioallantoic membrane

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：