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玛瑙红樱桃最佳愈伤组织诱导培养基的筛选

中南农业科技 2024年第4期45卷 19-22页

作者：常嗯鸣王春艳陈九同涂珍杰韩世明王月霞六盘水师范学院生物科学与技术学院贵州六盘水553000

为提高玛瑙红樱桃(Cerasus pseudocerasus Lindl.)愈伤组织诱导率,以MS培养基为基本培养基,采取单因素试验分别对0.1%HgCl_(2)处理外植体的时间、NAA浓度和iba浓度范围进行探究,再使用BoxBehnken响应面法进行三因素的优化试验,筛选玛瑙... 详细信息

为提高玛瑙红樱桃(Cerasus pseudocerasus Lindl.)愈伤组织诱导率,以MS培养基为基本培养基,采取单因素试验分别对0.1%HgCl_(2)处理外植体的时间、NAA浓度和iba浓度范围进行探究,再使用BoxBehnken响应面法进行三因素的优化试验,筛选玛瑙红樱桃的最佳愈伤组织诱导培养基。结果表明,单因素试验中,0.1%HgCl_(2)最佳处理时间为5 min,NAA浓度为0.35~0.65 mg/L,平均诱导率达73%,iba浓度为0.20~0.40 mg/L,平均诱导率达67%;Box-Behnken响应面模型分析得出最佳愈伤组织诱导培养基配方为MS+0.51 mg/L NAA+0.35 mg/L iba,0.1%HgCl_(2)处理外植体时间为4.5 min,平均诱导率达95%。NAA与iba配合使用效果优于单独使用。

关键词：玛瑙红樱桃(Cerasus pseudocerasus Lindl.) 愈伤组织诱导培养基 NAA iba 响应面分析筛选

Effects of indole-3-butyric acid and age of stem cuttings on root morphology,growth,and survival of Cornus sericea

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Journal of Forestry Research 2023年第2期34卷 433-440页

作者： Sahari Inoue Chibuzo Ilogu Jean-Marie Sobze Northern Alberta Institute of Technology Centre for Boreal ResearchPeace RiverAB T8S 1R2Canada Fine Phenotyping Lab Crop Health and ProtectionHarpenden AL52JQUK

Red osier dogwood(Cornus sericea L.),widely distributed throughout North America,is essential for wildlife,thus biodiversity.It is recommended for reclamation or revegetation of sites disturbed by oil and gas extraction because it tolerates a wide range of soil types and high pH levels.Since germination of this species is extremely difficult with long stratification requirements and poor germination,cuttings facilitate propagation.In this study,to develop techniques to propagate the species from stem cuttings,four concentrations of indole-3-butyric acid(iba)and two cutting ages previous year’s growth(PYG)and current year’s growth(CYG)were investigated for survival and growth.After 4 months,survival rate,height growth,and shoot biomass from PYG cuttings were enhanced with IB A.In contrast,IB A did not affect these parameters in CYG cuttings.Root morphology was significantly affected by IB A concentration and cutting age.iba,at higher concentrations,increased root surface and length.Total root surface area and length of PYG cuttings were increased,which may facilitate the absorption of essential resources and consequently increase growth.The results indicate that increasing the concentration of IB A may be an effective way to have better plant survival and growth of previous year’s cuttings in red osier dogwood.

关键词： Vegetative propagation iba Chronological age Cornus sericea L. Survival rate

Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第7期14卷 621-631页

作者： Anna Pick Kiong LING Kinn Poay TAN Sobri HUSSEIN Division of Human Biology International Medical University Department of Science Faculty of Engineering and Science Universiti Tunku Abdul Rahman Agrotechnology and Bioscience Division Malaysian Nuclear Agency

Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (iba) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. iba was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of iba, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs.

关键词：植物生长调节剂茎段 VAR 叶外植体愈伤组织自然栖息地培养基 iba

Altered relationship between thickness and intrinsic activity amplitude in generalized tonic–clonic seizures

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Science Bulletin 2016年第24期61卷 1865-1875页

作者： Wei Liao Jue Wang Ting Xu Zhiqiang Zhang Gong-Jun Ji Qiang Xu Zhengge Wang Fang Yang Xi-Nian Zuo Anqi Qiu Yu-Feng Zang Guangming Lu Huafu Chen Department of Medical Imaging Jinling Hospital Nanjing University School of Medicine Nanjing 210002 China Key Laboratory for Neuroinformation of Ministry of Education Center for Information in BioMedicine School of Life Scienceand Technology University of Electronic Science and Technology of China Chengdu 610054 China Department of Psychology School of Education Hangzhou Normal University Hangzhou 310015 China Center for Cognition and Brain Disorders and the Affiliated Hospital Hangzhou Normal University Hangzhou 310015 China Zhejiang Key Laboratory for Research in Assessment of Cognitive Impairments Hangzhou 310015 China Key Laboratory of Behavioral Science and Magnetic Resonance Imaging Research Center Institute of Psychology Chinese Academy of Sciences Beijing 100101 China Department of Neurology The First Affiliated Hospital of Anhui Medical University Hefei 230022 China Department of Radiology Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Nanjing 210002 China Department of Neurology Jinling Hospital Nanjing University School of Medicine Nanjing 210002 China Department of Biomedical Engineering and Clinical Imaging Research Center National University of Singapore Singapore 117583 Singapore

A thinner cortex has higher potential for binding GABA receptor A which is associated with larger amplitudes of intrinsic brain activity(i BA). However, the relationship between cortical thickness and i BA is unknown in intact and epileptic brains. To this end, we investigated the relationship between cortical thickness measured by highresolution MRI and surface-based i BA derived from resting-state functional MRI in normal controls(n = 82) andpatients with generalized tonic–clonic seizures(GTCS)only(n = 82). We demonstrated that the spatial distribution of cortical thickness negatively correlated with surface-based i BA amplitude at both whole-brain and within independent brain functional networks. In GTCS patients,spatial coupling between thickness and i BA amplitude decreased in the default mode, dorsal attention, and somatomotor networks. In addition, the vertex-wise acrosssubject thickness–i BA amplitude correspondence altered in the frontal and temporal lobes as well as in the precuneus in GTCS patients. The relationship between these two modalities can serve as a brain-based marker for detecting epileptogenic changes.

关键词：厚度测量幅度大脑活动 GABA受体功能网络 iba 高分辨率空间分布

The effects of climate and land use change on the potential distribution and nesting habitat of the Lesser Adjutant in Nepal

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Avian Research 2023年第3期14卷 326-335页

作者： Hem Bahadur Katuwal Hari Prasad Sharma Prashant Rokka Krishna Prasad Bhusal Bishnu Prasad Bhattarai Sabina Koirala Sandeep Chhetri Luitel Shailendra Yadav Ganesh Sah Hem Sagar Baral Laxman Prasad Poudyal Lin Wang Rui-Chang Quan Center for Integrative Conservation Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglaYunnan666303China Southeast Asia Biodiversity Research Institute Chinese Academy of SciencesYezinNay Pyi Taw05282Myanmar Yunnan International Joint Laboratory of Southeast Asia Biodiversity Conservation MenglaYunnan666303China Nepal Zoological Society Kathmandu44600Nepal Central Department of Zoology Institute of Science and TechnologyTribhuvan UniversityKathmandu44600Nepal Faculty of Forestry Agriculture and Forestry UniversityHetaudaMakwanpur44107Nepal IUCN SSC Bird Red List Authority Rue Mauverney1196GlandSwitzerland Mithila Wildlife Trust JanakpurdhamDhanusa45600Nepal Zoological Society of London-Nepal Office Kathmandu44600Nepal Institute of Land Water and SocietySchool of Environmental SciencesCharles Sturt UniversityAlbury-WodongaAustralia Shivapuri-Nagarjun National Park Department of National Parks and Wildlife ConservationKathmanduNepal

Climate change and land use change pose a threat to the world’s biodiversity and have significant impacts on the geographic distribution and composition of many bird species,but little is known about how they affect threatened large-sized waterbird species that rely on agricultural landscapes.To address this gap,we investigated how climate and land use changes influence the distribution and nesting habitats of the globally vulnerable Lesser Adjutant(Leptoptilos javanicus) in Nepal.Between 2012 and 2023,we collected distribution data from 24 districts and nesting site information from 18 districts.In a nation-wide breeding survey conducted in 2020,we documented a total of 581 fledglings from 346 nests in 109 colonies.The ensemble model predicted a current potential distribution of 15%(21,637 km2) and a potential nesting habitat of 13%(19,651 km2) for the species in Nepal.The highest predicted current suitable distribution and nesting habitat was in Madhesh Province,while none was predicted in Karnali Province.The majority of this predicted distributional and nesting habitat falls on agricultural landscapes(>70%).Our model showed a likely range expansion of up to 15%(21,573 km2) for the distribution and up to 12%(17,482 km2) for the nesting habitat under SSP5-8.5 scenarios for the 2070s.The range expansion is expected to occur mainly within the current distribution and breeding range(Tarai and some regions of Siwalk),particularly in Lumbini and Sudurpashchim provinces,and extend to the northern portions(Siwalik and Mid-hill regions) in other provinces.However,the current Protected Areas and Important Bird and Biodiversity Areas are inadequate for providing optimal habitats for the species.Although the model suggests range expansion,the use of such novel habitats is primarily contingent on the availability and protection of large-sized trees(particularly Bombax ceiba,observed in 65% of colonies) in agricultural regions where nesting occurs.Therefore,our research suggests that agricultural landscapes should be priori

关键词： Agricultural landscapes Bombax ceiba Ensemble modeling Farmland bird iba Nest site

Integrated assessment of adventitious rhizogenesis in Eucalyptus : root quality index and rooting dynamics

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Journal of Forestry Research 2020年第6期31卷 2145-2161页

作者： Romeet Saha Harish Singh Ginwal Girish Chandra Santan Barthwal Division of Genetics and Tree Improvement Forest Research InstituteDehra DunUttarakhand 248195India Statistics Division ICFREForest Research InstituteDehra DunUttarakhand 248006India

Clonal propagation of eucalypts has considerable importance due to the increasing demands for short rotation tree crops.Rooting quality is important as it governs the soil exploitation capacity of the plant and the anchorage of trees which are susceptible to wind damage.This study assesses the quality of adventitious rooting by coppice cuttings of commercially important Eucalyptus clones using multiple attribute ranking of the diff erences in parameters of root growth.The eff ects of diff erent concentrations of indole-3-butyric acid(iba)on root development were observed.Cuttings were treated with 500,1000,4000,6000 mg L−1 iba and tannic acid for 10 s,2 h and 24 h.Total length of root systems,number of roots,shoot to root ratios,number of root segments,extent of forking,rooting percentage,average root diameters,and number of root tips were measured.Grey relational analysis was used to create a comparability sequence to rank treatments.Reducing the concentration of auxin(iba)and increasing the length of exposure produced better quality roots in Eucalyptus camaldulensis and interspecifi c hybrids(reciprocal hybrids of E.tereticornis and E.camaldulensis),while the opposite was observed with E.tereticornis clones.A root quality index was proposed,based on the Dickson quality index for the assessment of root system characteristics and considered total mass,shoot:root ratios,total length of root systems,and average root diameters.It has the advantage of implementation convenience.A positive correlation was obtained between grey relational analysis grades and root quality index.Rooting dynamics were studied by evaluating the total length of the root system at seven-day intervals and plotting daily current and medium increments using regression analysis.The curves showed the variation in growth rates among the diff erent clones,and their intersection gave the optimal time of permanence(time at which further growth is restricted)which varied considerably.The highest daily current increment was 35–40 days for all clones.

关键词： iba Eucalyptus Rooting dynamics Root quality Grey-relational analysis

外源生长素质量浓度和扦插基质对色木槭嫩枝扦插生根的影响

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森林工程 2022年第5期38卷 1-9页

作者：周鑫鑫李茹月臧世阳堃芃杨玲林木遗传育种国家重点实验室(东北林业大学) 哈尔滨150040

研究外源生长素(NAA和iba)质量浓度和不同基质类型对色木槭嫩枝插穗生根指标和生根质量的影响,筛选出最佳的激素质量浓度和基质,为色木槭优良种质资源扩大繁殖和推广栽培奠定基础。本研究以色木槭当年生半木质化插穗为试验材料,设定0、... 详细信息

研究外源生长素(NAA和iba)质量浓度和不同基质类型对色木槭嫩枝插穗生根指标和生根质量的影响,筛选出最佳的激素质量浓度和基质,为色木槭优良种质资源扩大繁殖和推广栽培奠定基础。本研究以色木槭当年生半木质化插穗为试验材料,设定0、500、1 000、1 500、2 000 mg/L 5种NAA与iba组合溶液(NAA+iba)的质量浓度,选用蛭石、泥炭土、珍珠岩、珍珠岩+蛭石4种基质进行单因素随机区组试验。结果表明,不同NAA+iba质量浓度和基质类型对测量指标均有极显著的影响(Piba质量浓度和不同基质类型影响显著,综合考虑生根效果和生根质量,1 500 mg/L为最佳NAA+iba激素质量浓度;珍珠岩在生根指标上显著优于其他基质,为最优选择;育苗后期为追求生根质量,则应使用珍珠岩+蛭石作为栽培基质。该研究建立的色木槭嫩枝扦插方法可用于优良种质资源的扩大繁殖。

关键词：色木槭半木质化枝条扦插繁殖生长素质量浓度 NAA iba 基质

氢氧化钠、温水和iba浸种对华山松幼苗生长和生物量的影响

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江西农业大学学报 2022年第5期44卷 1156-1168页

作者：李小军李莲芳顾梦张合瑶刘娴周冬梅侯海雄西南林业大学林学院云南昆明650224

【目的】为了解不同浓度氢氧化钠(NaOH)与温水和吲哚丁酸(Indolebutric acid,iba)溶液组合浸种对华山松(Pinus armandi)幼苗生长和生物量积累的影响,提供华山松壮苗培育种子处理的科学依据。【方法】采用L_(9)(3~4)正交设计,开展NaOH、... 详细信息

【目的】为了解不同浓度氢氧化钠(NaOH)与温水和吲哚丁酸(Indolebutric acid,iba)溶液组合浸种对华山松(Pinus armandi)幼苗生长和生物量积累的影响,提供华山松壮苗培育种子处理的科学依据。【方法】采用L_(9)(3~4)正交设计,开展NaOH、温水和iba溶液组合浸种对华山松幼苗生长和生物量影响的试验研究,测定苗龄128 d幼苗的生长和生物量,计算含水率,分析指标间的相关性,推导指标间的线性方程。【结果】苗龄128 d时,处理组合幼苗的地径、胚轴长、苗高、主根长、全株生物量和含水率分别为1.52~2.04 mm、6.0~8.8 cm、7.0~9.6 cm、14.1~22.8 cm、0.236 7~0.314 1 g/株和67.9%~72.3%,处理组合间6个指标均呈极显著的差异(Piba溶液浸种外,其余指标的均是NaOH溶液浸种。0.15%的NaOH溶液浸种极显著地促进华山松幼苗地径的增大和全株生物量的积累(Piba溶液浸种极显著和显著地促进胚轴和苗高的生长(Piba溶液浸种的浓度间呈极显著或显著的正相关(Piba溶液的不同水平组合,生产中可促进华山松幼苗生长和生物量积累,并改变其器官和全株含水率,可根据苗木培育的目标需求,采用不同水平组合培育幼苗,实现其壮苗培育的目的。促进地径生长和全株生物量积累,可采用0.15%NaOH溶液浸种1 h后用温水浸种4 h;温水浸种4 h后0.30 g/L的iba溶液浸种3 h可促进胚轴伸长和苗高生长;不处理的主根最长(ck);0.30%NaOH溶液浸种1 h后0.30 g/Liba溶液浸种3 h提高全株含水率。在生产实践中,可根据实际需要,采用不同水平组合培育华山松幼苗。

关键词：华山松 NaOH 温水 iba 幼苗生长生物量

Vegetative propagation of Litsea monopetala, a wild tropical medicinal plant: Effects of indole-3-butyric acid (iba) on stem cuttings

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Journal of Forestry Research 2011年第3期22卷 409-416页

作者： Tarit Kumar Baul Mohammad Mosharraf Hossain Mohammad Mezbahuddin Mohammed Mohiuddin School of Environment Natural Resources and Geography Bangor University Bryn Eithin Room K35 St. Mary's Site Bangor LL57 1DZ UK. Institute of Forestry and Environmental Sciences University of Chittagong Chittagong 4331 Bangladesh Research Fellow Graduate School of Engineering Osaka University Suita Osaka 565-0871 Japan Department of Renewable Resources University of Alberta Canada Professor Institute of Forestry and Environmental Sciences University of Chittagong Chittagong 4331 Bangladesh

In this study we investigated the rooting ability and the growth performance of juvenile single-node leafy stem cuttings of Litsea monopetala （Roxb） Pers. collected from two mature mother trees preserved in the hill forest of Chittagong district, Bangladesh. The rooting ability of cuttings was studied under 0%, 0.1%, 0.2% and 0.4% indole-3-butyric acid （iba） treatments. Significantly better rooting response （p ≤ 0.05） was observed with 0.1% iba compared to control （0% iba）. The mean number of roots and the length of the longest root of cuttings in different treatments showed no significant difference （p≤0.05）. After transfer into polythene bags from non-mist propagator, rooted cuttings treated with 0%, 0.1% and 0.2% iba demonstrated the highest （100 ± 0.00%） survival capacity. The mean number of shoots developed in cuttings in the polythene bags in first three weeks varied significantly （p≤0.05） among the treatments. Effects of three fertilizer treatments, viz. T0 （no fertilizer）, T1 （10g Urea, 20g TSP, 10g MOP dissolved in 1 L water） and T2 （10g Urea, 20g TSP, 10g MOP dissolved in 2 L water） on initial growth ofstecklings were also measured over a 90-days period. The increment of leaf area of stecklings was significantly higher （p≤0.05） under T0 compared with that under T1 and T2 while the increment of stem length, collar diameter and root biomass varied insignificantly among different fertilizer treatments. The results suggest that rooting juvenile single-node leafy stem cuttings could be an effective mean of regenerating L. monopetala. The application of 0.1% iba concentration is recommended for rooting of juvenile leafy stem cuttings and application of fertilizer appeared unnecessary for the subsequent growth of stecklings in polythene bags.

关键词： iba Litsea monopetala non-mist propagator rooting stem cutting