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Hyperbaric oxygen treatment on keloid tumor immune gene expression

Chinese Medical Journal 2021年第18期134卷 2205-2213页

作者： Chun-Hu Wang Meng-Jie Shan Hao Liu Yan Hao Ke-Xin Song Huan-Wen Wu Tian Meng Cheng Feng Zheng Qi Zhi Wang You-Bin Wang 17th Department of Plastic Surgery Plastic Surgery HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100144China Department of Plastic Surgery Peking Union Medical College HospitalBeijing 100730China Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730China Department of Pathology Peking Union Medical College HospitalBeijing 100730China

Background:Hyperbaric oxygen treatment(HBOT)has been demonstrated to influence the keloid recurrence rate after surgery and to relieve keloid symptoms and other pathological processes in keloids.To explore the mechanism of the effect of HBOT on keloids,tumor immune gene expression and immune cell infiltration were studied in this work.Methods:From February 2021 to April 2021,HBOT was carried out on keloid patients four times before surgery.Keloid tissue samples were collected and divided into an HBOT group(keloid with HBOT before surgery[HK]group,n=6)and a non-HBOT group(K group,n=6).Tumor gene expression was analyzed with an Oncomine Immune Response Research Assay kit.Data were mined with R package.The differentially expressed genes between the groups were compared.Hub genes between the groups were determined and verified with Quantitative Real-time PCR.Immune cell infiltration was analyzed based on CIBERSORT deconvolution algorithm analysis of gene expression and verified with immunohistochemistry(IHC).Results:Inflammatory cell infiltration was reduced in the HK group.There were 178 upregulated genes and 217 downregulated genes.Ten hub genes were identified,including Integrin Subunit Alpha M(ITGAM),interleukin(IL)-4,IL-6,IL-2,Protein Tyrosine Phosphatase Receptor Type C(PTPRC),CD86,transforming growth factor(TGF),CD80,CTLA4,and IL-10.CD80,ITGAM,IL-4,and PTPRC with significantly downregulated expression were identified.IL-10 and IL-2 were upregulated in the HK group but without a significant difference.Infiltration differences of CD8 lymphocyte T cells,CD4 lymphocyte T-activated memory cells,and dendritic resting cells were identified with gene CIBERSORT deconvolution algorithm analysis.Infiltration levels of CD4 lymphocyte T cell in the HK group were significantly higher than those of the K group in IHC verification.Conclusion:HBOT affected tumor gene expression and immune cell infiltration in keloids.CD4 lymphocyte T cell,especially activated memory CD4+T,might be the key regulatory immune cell,and its related

关键词： Keloid Hyperbaric oxygen treatment Tumor immune gene Immune cell

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Hyperbaric oxygen treatment Improves Hearing Level via Attenuating TLR4/NF-κB Mediated Inflammation in Sudden Sensorineural Hearing Loss Patients

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Biomedical and Environmental Sciences 2020年第5期33卷 331-337页

作者： LIU Xue Hua LIANG Fang JIA Xing Yuan ZHAO Lin ZHOU Yan YANG Jing Department of hyperbaric oxygen Medicine Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Institute of Basic Medicine Sciences Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Department of hyperbaric oxygen Medicine Beijing Pinggu HospitalBeijing 101200China

Objective Hyperbaric oxygen treatment(HBOT)has demonstrated efficacy in improving hearing levels of patients with idiopathic sudden sensorineural hearing loss(ISSHL);however,the underlying mechanisms are not well understood.HBOT alleviates the inflammatory response,which is mediated by Toll-like receptor(TLR)4 and nuclear factor(NF)-κB.In this study we investigated whether HBOT attenuates inflammation in ISHHL patients via alteration of TLR4 and NF-κB expression.Methods ISHHL patients(n=120)and healthy control subjects(n=20)were enrolled in this study.Patients were randomly divided into medicine group treated with medicine only(n=60)and HBO group receiving both HBOT and medicine(n=60).Audiometric testing was performed pre-and posttreatment.TLR4,NF-кB,and TNF-αexpression in peripheral blood of ISSHL patients and healthy control subjects was assessed by ELISA before and after treatment.Results TLR4,NF-κB,and TNF-αlevels were upregulated in ISSHL patients relative to healthy control subjects;the levels were decreased following treatment and were lower in the HBO group than that in the medicine group post-treatment(P<0.05 and P<0.01).Conclusion HBOT alleviates hearing loss in ISSHL patients by suppressing the inflammatory response induced by TLR4 and NF-κB signaling.

关键词： Hyperbaric oxygen treatment Sudden sensorineural hearing loss Toll-like receptor 4 Nuclear factor-kB

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Overcoming ischemia in the diabetic foot:Minimally invasive treatment options

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World Journal of Diabetes 2021年第12期12卷 2011-2026页

作者： Stavros Spiliopoulos Georgios Festas Ioannis Paraskevopoulos Martin Mariappan Elias Brountzos Second Department of Radiology Interventional Radiology UnitAttikon University HospitalAthens 12461Greece Department of Clinical Radiology Interventional Radiology UnitAberdeen Royal InfirmaryNHS GrampianAberdeen AB252ZNUnited Kingdom Second Department of Radiology School of MedicineNational and Kapodistrian University of AthensAthens 12461Greece

As the global burden of diabetes is rapidly increasing,the incidence of diabetic foot ulcers is continuously increasing as the mean age of the world population increases and the obesity epidemic advances.A significant percentage of diabetic foot ulcers are caused by mixed micro and macro-vascular dysfunction leading to impaired perfusion of foot tissue.Left untreated,chronic limb-threatening ischemia has a poor prognosis and is correlated with limb loss and increased mortality;prompt treatment is required.In this review,the diagnostic challenges in diabetic foot disease are discussed and available data on minimally invasive treatment options such as endovascular revascularization,stem cells,and gene therapy are examined.

关键词： Diabetic foot Peripheral artery disease Critical limb ischemia Endovascular revascularization techniques Gene and stem cells delivery Hyperbaric oxygen treatment

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The analysis of therapeutic effect of hyperbaric oxygen on traumatic cerebral infarction and change in platelet parameters

The analysis of therapeutic effect of hyperbaric oxygen on t...

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2011中华医学会神经外科学学术会议

作者： Guangxin Chen1 Lunshan Xu1 Min hui Xu1 1.Department of Neurosurgery Daping Hospital,The third Military Medical university Chonqing 4 0004 2

objectives to discuss the mechanism and clinical manifestation of traumatic cerebral infarction(TCI), the therapeutic effect of hyperbaric oxygen on TCI and change in platelet parameter(PLT), mean platelet volume(MPV)... 详细信息

关键词： Traumatic cerebral infarction PLT MPV PDW Hyperbaric oxygen treatment prognosi

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