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Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

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Natural Science 2009年第1期1卷 47-54页

作者： Jun-Jun Zhu Qiang Yong Yong Xu Shang-Xing Chen Shi-Yuan Yu College of Forestry Jiangxi Agricultural University Nanchang 330045 China. Ministry of Education Key Laboratory of Forest Genetics & Biotechnology Nanjing Forestry University Nanjing 210037 China Ministry of Education Key Laboratory of Forest Genetics & Biotechnology Nanjing Forestry University Nanjing 210037 China

Yeast Pichia stipitis CBS 5776 was developed through adaptation fermentation step by step in steam exploded corn stover prehydrolyzate because high concentration of weak acids and other inhibitors present in the prehydrolyzate could degrade the fermentability. However, the adaptability of Pichia stipitis CBS 5776 in the prehydrolyzate was so limited that steam strip-ping and overliming were applied to remove these inhibitors from it. Corn stover was steam exploded;the filtrate of steam exploded corn stover was hydrolyzed with dilute sulfuric acid, and then the acid hydrolyzate was detoxified and fermented by Pichia stipitis CBS 5776. Steam stripping could remove volatile com-pounds from the acid hydrolyzate and the fil-trate. At a steam stripping time of 120min, 81% acetic acid and 59% formic acid were removed from the acid hydrolyzate, 77% acetic acid and 45% formic acid were removed from the filtrate, while furfural was stripped off completely from the acid hydrolyzate and the filtrate. Overliming could reduce the contents of furfural and phe-nolics present in the acid hydrolyzate, however, sugars, especially pentoses, were also removed partially. It was necessary to detoxify the acid hydrolyzate in order to ferment the sugars to ethanol. Acid hydrolyzate detoxified with a combination of steam stripping for 120 min and overliming at pH11 and 60℃ for 90 min, its fer-mentability was significantly improved. Xylose was consumed nearly completely in 24h with an ethanol yield of 15.92g/l, 80.34% of theoretical.

关键词： Steam Explosion Steam Stripping Overliming Inhibitor Acid hydrolyzate

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Identification and activity evaluation of Astragalus Radix from different germplasm resources based on specific oligosaccharide fragments

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Chinese Herbal Medicines 2021年第1期13卷 33-42页

作者： Shu-ying Li Di Wang Xian-rong Li Xue-mei Qin Yu-guang Du Ke Li National Glycoengineering Research Center Shandong Key Laboratory of Carbohydrate Chemistry and GlycobiologyShandong UniversityQingdao 266237China Modern Research Center for Traditional Chinese Medicine Shanxi UniversityTaiyuan 030006China College of Chemistry and Chemical Engineering Shanxi UniversityTaiyuan 030006China Shanxi Jianshuo Food and Drug Research Institute Co. Ltd.Taiyuan 030000China Shanxi Academy of Traditional Chinese Medicine Taiyuan 030000China Institure of Process Engineering Chinese Academy of SciencesBeijing 100190China

Objective: Based on trifluoroacetic acid(TFA) hydrolysis, polyacrylamide gel electrophoresis(PAGE) and high performance thin layer chromatography(HPTLC) analysis, the carbohydrate responsible for immunomodulatory activity are used as quality indicators for Astragalus Radix(AR).Methods: In this study, 24 batches of AR from different germplasm resources were selected as the research object, and AR polysaccharides were extracted. PAGE and HPTLC methods were used to analyze the partial acid hydrolyzate of AR polysaccharides and obtain a series of saccharide fingerprints. The data were analyzed by principal component analysis to obtain the difference between AR from different germplasm ***: The results showed that trisaccharide and tetrasaccharide could be used as differential fragments to distinguish AR of different cultivation methods;Disaccharides and trisaccharides can be used as differential fragments to distinguish different species of AR. The immunological activity analysis of the specific oligosaccharide fragment of AR showed that the specific oligosaccharide fragment of AR could promote the secretion of TNF-α, IL-1β, IL-6, and NO in THP-1 cells in a concentration-dependent ***: Both PAGE and HPTLC methods can be used to evaluate AR from different germplasm resources. This study laid the foundation for the quality evaluation of AR medicinal herbs.

关键词： Astragalus Radix germplasm resources hydrolyzate polysaccharide saccharide fingerprints

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