检索结果-南通市图书馆

hydrogen sulfide Attenuates High Glucose-induced Myocardial Injury in Rat Cardiomyocytes by Suppressing Wnt/beta-catenin Pathway

引用

Current Medical Science 2019年第6期39卷 938-946页

作者： Min ZHANG Mao YE Department of Endocrinology the Central Hospital of Enshi Autonomous PrefectureEnshi Clinical College of Wuhan UniversityEnshi 445000China

Diabetic cardiomyopathy(DCM)is one of the major heart complications of diabetic patients.hydrogen sulfide(H2S)is now recognized as an important signaling molecule and has been shown to attenuate the development of diabetic cardiomyopathy.However,the underlying mechanisms linking H2S and the development of DCM have not been fully elucidated.In the present study,we therefore sought to explore the role and mechanism of H2S in the pathogenesis of DCM by establishing high glucose-induced injury model in neonatal rat cardiomyocytes(NRCMs)and H9c2 cells.Using cystathionine gamma-lyase(CSE)overexpression and CSE interference vectors transfection,the cell viability,cell apoptosis,and oxidative stress were determined and compared between the treatment of high glucose induction and exgenous NaHS administration.Meanwhile,the relationship between the CSE/H2S system and Wnt/beta-catenin pathway was analyzed and discussed in the high glucose-induced cardiomyocytes.Our results indicated that H2S played an important protective role in high glucose-induced apoptosis and oxidative stress in cardiomyocytes,as shown by the decreased reactive oxygen species and malondialdehyde levels,and the increased activities of superoxide dismutase,catalase and glutathione peroxidase.Moreover,H:S could attenuate the Wnt/p-catenin signalling pathway and up-regulate the expression of haem oxygenase-1(HO-1)and NAD(P)H:quinone oxidoreductase 1(NQOl)in the diabetic myocardium cells.Together,these results demonstrated that H2S could attenuate high glucoseinduced myocardial injury in rat cardiomyocytes by suppressing Wnt/p-catenin pathway.

关键词： hydrogen sulfide Wnt/p-catenin diabetic cardiomyopathy high glucose

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

hydrogen sulfide: the shutter button of stomata in plants

引用

Science China(Life Sciences) 2016年第11期59卷 1187-1188页

作者： Zhuping Jin Yanxi Pei School of Life Science Shanxi University

In the past few decades,hydrogen sulfide(H2S)has been newly witnessed as a third gasotransmitter with similar biological functions to its two previously identified counterparts nitric oxide(NO)and carbon monoxide(CO),... 详细信息

关键词： hydrogen sulfide the shutter button of stomata in plants

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

hydrogen sulfide Detection Using a Gold Nanoparticle/Metalloprotein Based Probe

引用

Chinese Physics Letters 2014年第8期31卷 170-173页

作者： MeisamOmidi FaeultyofNewScienceandTechnologyUniversityofTehran TehranIran ResearchCenterforNewTechnologiesinLife-ScienceEngineering UniversityofTehranTehranIran SchoolofBiology CollegeofScienceUniversityofTehranTehranIran NanobiomedicineCenterofExcellance UniversityofTehranTehranIran

We present a simple method for direct detection of hydrogen sulfide （H2S） in an aqueous solution. This method represents a novel biosensor based on metalloprotein cytoehrome c （cyt c） with the localized surface plasmon resonance of gold nanoparticles （AuNPs）. For this purpose, we develop a new approach based on attaching chemically-modified cyt e onto AuNPs. Here, by reacting H2S with protein heine center, its conformation changes in the locality of the heine moiety. The eonformational changes occurring in the protein alter the spectral characteristics by changing the dielectric properties of AuNPs. The conformational changes of eyt c induced by the H2S interaction are characterized by the UV-visible absorption spectroscopy and the circular dichroism technique. The limit of the detection and sensitivity of the AuNPs/cyt c biosensor are evaluated by using UV- visible spectroscopy. According to the experiments, it is revealed that H2S can be detected at a concentration of 4.0μM （1.3ppb） by the fabricated AuNPs/cyt c biosensor. In addition, the sensor retains activity and gives reproducible results after storage in 4℃ for 60 d. This simple and cost-effective sensing platform provides a rapid and convenient detection for H2S at concentrations far below the hazardous limit.

关键词： hydrogen sulfide

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

hydrogen sulfide inhibits beta-amyloid peptide-induced apoptosis in PC12 cells and the underlying mechanisms

引用

Neural Regeneration Research 2008年第9期3卷 939-944页

作者： Xiuqin Chen Jingtian Li Jinhui Zou Bailing Li Meng Wang Department of Physiology Medical College of Shaoguan University Shaoguan 512026 Guangdong Province China

BACKGROUND：Studies have demonstrated that hydrogen sulfide（H2S） levels are 55% lower in brains of Alzheimer＇s disease（AD） patients than in age-matched normal individuals,which suggests that H2S might be involved in some aspects of AD pathogenesis.OBJECTIVE：To observe the protective mechanisms of varied concentrations of H2S against β-amyloid-peptide（Aβ） induced apoptosis in pheochromoytoma（PC12） cells,and to analyze the pathway of action.DESIGN,TIME AND SETTING：A controlled,observational,in vitro experiment was performed at Neurophysiology Laboratory in Zhongshan Medical School,Sun Yat-sen University between July 2006 and May 2007.MATERIALS：PC12 cells were provided by the Animal Experimental Center of Medical School of Sun Yat-sen University.Glybenclamide,rhodamine123,and dihydrorhodamine123 were purchased from Sigma（USA）.METHODS：PC12 cells were incubated at 37 ℃ in a 5% CO2-enriched incubator with RPMI-1640 medium,supplemented with 5% horse-serum and 10% fetal bovine serum.Cells in logarithmic growth curves received different treatment：The PC12 cells were maintains at 37 ℃ with the original medium,then incubated in Aβ25-35,sodium hydrosulfide（NaHS）,glybenclamide,NaHS＋ Aβ25-35,or pretreated with glybenclamide 30 minutes prior to administration of and Aβ25-35,respectively.MAIN OUTCOME MEASURES：（1） The survival rate of PC12 cells was detected by MTT assay and Hoechst staining.（2） The apoptosis rate of PC12 cells was detected utilizing flow cytometry with propidium iodide staining,and morphological changes of apoptotic cells were observed.（3） The mitochondrial membrane potential was detected by Rhodamine123-combined flow cytometry.（4） The intracellular reactive oxygen species content was detected by dihydrorhodamine123-combined flow cytometry.RESULTS：Aβ25-35 induced significantly decreased viability and increased percentage of apoptosis in PC12 cells,as well as dissipated mitochondrial membrane potential expression and an overproduction of reactive oxygen species.When PC12 cells were co-treated with NaHS and Aβ25-35

关键词： apoptosis β -amyloid peptide cytoprotection hydrogen sulfide mitochondrial membrane potential reactive oxygen species

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

hydrogen sulfide regulates vascular endoplasmic reticulum stress in apolipoprotein E knockout mice

引用

Chinese Medical Journal 2011年第21期124卷 3460-3467页

作者： CHEN Zhi-fang ZHAO Bin TANG Xiu-ying LI Wei ZHU Lu-lu TANG Chao-shu DU Jun-bao JIN Hong-fang Department of Emergency Medicine Beijing Jishuitan Hospital Beijing 100035 China Department of Electron Microscope Peking University First Hospital Beijing 100034 China Department of Pediatrics Peking University First Hospital Beijing 100034 China Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100034 China Key Laboratory of Molecular Cardiovascular Sciences Ministry of Education Beijing 100191 China

Background Atherosclerosis is an important cardiovascular disease, becoming a major and increasing health problem in developed countries. However, the possible underlying mechanisms were not completely clear. In 2009, our research group first discovered that hydrogen sulfide （H2S） as a novel gastrotransmitter played an important anti-atherosclerotic role. The study was designed to examine the regulatory effect of hydrogen sulfide （H2S） on endoplasmic reticulum stress （ERS） in apolipoprotein E knockout （apoE/） mice fed a Western type diet. Methods C57BL/6 mice and homozygous apoE/ mice were fed a Western type diet. C57BL/6 mice were injected intraperitoneally with normal saline （5 ml/kg per day） as control group. The apoE/ mice were treated with the same dose of normal saline as the apoE/ group, injected intraperitoneally with sodium hydrosulfide （NariS, an H2S donor, 56 IJmol/kg per day） as the apoE/＋NaHS group and injected intraperitoneally with DL-propargylglycine （PPG, a cystathionine-y-lyase inhibitor, 50 mg/kg, per day） as the apoE/ ＋PPG group. After 10 weeks, the mice were sacrificed and the plasma lipids were detected. Sections of aortic root from these animals were examined for atherosclerotic lesions by HE and oil red O staining. The aortic ultrastructure and microstructure were analyzed with the help of light and electronic microscope. Glucose-regulated protein 78 （GRP78）, caspase-12, copper-andzinc-containing superoxide dismutase （Cu/ZnSOD） and Mn-containing superoxide dismutase （MnSOD） protein expression in aortic tissues were detected with immunohistochemistry. The level of intracellular reactive oxygen species （ROS） were measured by using a commercial assay kit. Results Compared with control mice, apoE/ mice showed increased plasma levels of total cholesterol （TC）, triglyceride （TG） and low density lipoprotein （LDL）, decreased high density lipoprotein （HDL）, increased aortic plaque size, destroyed ultra-structure of aortic tissue, and increased expression of GRP78 and caspase-12 proteins. Compared w

关键词： hydrogen sulfide atherosclerosis endoplasmic reticulum stress

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

hydrogen sulfide and nervous system regulation

引用

Chinese Medical Journal 2011年第21期124卷 3576-3582页

作者： ZHOU Cheng-fang TANG Xiao-qing Department of Physiology Institute of Cognition and Nervous Systems Disease Medical College University of South China Hengyang Hunan 421001 China

Objective This review discusses the current status and progress in studies on the roles of hydrogen sulfide （H2S） in regulation of neurotoxicity, neuroprotection, and neuromodulator, as well as its therapeutic potential for neurodegenerative disorders. Data sources The data used in this review were mainly from Medline and PubMed published in English from 2001 to August 2011. The search terms were ＂hydrogen sulfide＂, ＂neuron＂, and ＂neurodegenerative disorders＂. Study selection Articles regarding the regulation of neuronal function, the protection against neuronal damage and neurological diseases, and their possible cellular and molecular mechanisms associated with H2S were selected. Results The inhibited generation of endogenous H2S is implicated in 1-methy-4-phenylpyridinium ion, 6-OHDA, and homocysteine-triggered neurotoxicity. H2S elicits neuroprotection in Alzheimer＇s disease and Parkinson＇s disease models as well as protecting neurons against oxidative stress, ischemia, and hypoxia-induced neuronal death. H2S offers anti-oxidant, anti-inflammatory and anti-apoptotic effects, as well as activates ATP-sensitive potassium channels and cystic fibrosis transmembrane conductance regulator CI channels. H2S regulates the long-term potentiation （LTP） and GABAB receptors in the hippocampus, as well as intracellular calcium and pH homeostasis in neurons and gila cells. Conclusions These articles suggest that endogenous H2S may regulate the toxicity of neurotoxin. H2S not only acts as a neuroprotectant but also serves as a novel neuromodulator.

关键词： hydrogen sulfide neuromodulator neuroprotectant

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

hydrogen sulfide protects against amyloid beta-peptide induced neuronal injury via attenuating inflammatory responses in a rat model

引用

The Journal of Biomedical Research 2013年第4期27卷 296-304页

作者： Hao Fan YuGuo Xiaoyan Liang Yibiao Yuan Xiaohong Qi Min Wang Jianhua Ma Hong Zhou Laboratory Center for Basic Medical Sciences Nanjing Medical University Department of Pathophysiology Nanjing Medical University Department of Endocrinology Nanjing First Hospital Nanjing Medical University

Neuroinflammation has been recognized to play a critical role in the pathogenesis of Alzheimer＇s disease （AD）, which is pathologically characterized by the accumulation of senile plaques containing activated microglia and amyloid β-peptides （Aβ）. In the present study, we examined the neuroprotective effects of hydrogen sulfide （H2S） on neuroinflammation in rats with Aβ1-40 hippocampal injection. We found that Aβ-induced rats exhibited a disorder of pyramidal cell layer arrangement, and a decrease of mean pyramidal cell number in the CA1 hippocampal region compared with those in sham operated rats. NaHS （a donor of H2S, 5.6 mg/kg/d, i.p.） treatment for 3 weeks rescued neuronal cell death significantly. Moreover, we found that H2S dramatically suppressed the release of TNF-α, IL-1β and IL-6 in the hippocampus. Consistently, both immunohistochemistry and Western blotting assays showed that H2S inhibited the upregulation of COX-2 and the activation of NF-κB in the hippocampus. In conclusion, our data indicate that H2S suppresses neuroinflammation via inhibition of the NF-κB activation pathway in the Aβ-induced rat model and has potential value for AD therapy.

关键词： Alzheimer＇s disease hydrogen sulfide cyclooxygenase-2 nuclear factor-κB （NF-κB） amyloid

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cellular preconditioning and mesenchymal stem cell ferroptosis

引用

World Journal of Stem Cells 2024年第2期16卷 64-69页

作者： Doaa Hussein Zineldeen Mazhar Mushtaq Khawaja Husnain Haider Basic Sciences Sulaiman AlRajhi UniversityAlbukairiyah 52736AlQaseemSaudi Arabia Department of Medical Biochemistry and Molecular Biology Faculty of MedicineTanta UniversityTanta 6632110Egypt

In this editorial,we comment on the article published in the recent issue of the World Journal of Stem Cells.They focus on stem cell preconditioning to prevent ferroptosis by modulating the cystathionineγ-lyase/hydrogen sulfide(H_(2)S)pathway as a novel approach to treat vascular disorders,particularly pulmonary hypertension.Preconditioned stem cells are gaining popularity in regenerative medicine due to their unique ability to survive by resisting the harsh,unfavorable microenvironment of the injured tissue.They also secrete various paracrine factors against apoptosis,necrosis,and ferroptosis to enhance cell survival.Ferroptosis,a regulated form of cell death characterized by iron accumulation and oxidative stress,has been implicated in various pathologies encompassing dege-nerative disorders to cancer.The lipid peroxidation cascade initiates and sustains ferroptosis,generating many reactive oxygen species that attack and damage multiple cellular structures.Understanding these intertwined mechanisms provi-des significant insights into developing therapeutic modalities for ferroptosis-related diseases.This editorial primarily discusses stem cell preconditioning in modulating ferroptosis,focusing on the cystathionase gamma/H_(2)S ferroptosis pathway.Ferroptosis presents a significant challenge in mesenchymal stem cell(MSC)-based therapies;hence,the emerging role of H_(2)S/cystathionase gamma/H_(2) S signaling in abrogating ferroptosis provides a novel option for therapeutic intervention.Further research into understanding the precise mechanisms of H_(2)S-mediated cytoprotection against ferroptosis is warranted to enhance the thera-peutic potential of MSCs in clinical settings,particularly vascular disorders.

关键词： Cell survival Cell therapy hydrogen sulfide Ferroptosis Preconditioning Stem cells Umbilical cord

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

hydrogen sulfide induces apoptosis of pulmonary artery smooth muscle cell in rats with pulmonary hypertension induced by high pulmonary blood flow

引用

Chinese Medical Journal 2009年第24期122卷 3032-3038页

作者： LI Wei JIN Hong-fang LIU Die SUN Jing-hui JIAN Pei-jun LI Xiao-hui TANG Chao-shu DU Jun-bao Department of Pediatrics Peking University First Hospital Beijing 100034 China Department of Pediatrics Jilin University First Hospital Changchun Jilin 130021 China Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100191 China Key Laboratory of Molecular Cardiovascular Sciences Ministry of Education Beijing 100191 China

Background Abnormal apoptosis of pulmonary artery smooth muscle cells （PASMCs） is an important pathophysiological process in the pulmonary artery structural remodeling and pulmonary hypertension. We investigated possible effect of endogenous hydrogen sulfide （H2S） on apoptosis of PASMCs during the development of pulmonary hypertension induced by high pulmonary blood flow. Methods Thirty-nine male Sprague-Dawley rats were randomly assigned to 4-week control, 4-week shunt, 4-week shunt＋propargylglycine （PPG）, 11-week control, 11-week shunt and 11-week shunt＋sodium hydrosulfide （NariS） groups. Rats in 4-week shunt, 4-week shunt＋PPG, 11-week shunt and 11-week shunt＋NariS groups underwent an abdominal aorta-inferior vena cava shunt. Rats in 4-week shunt＋PPG group were intraperitoneally injected with PPG, an inhibitor of endogenous H2S production, for 4 weeks. Rats in 11-week shunt＋NariS group were intraperitoneally injected with NariS, a H2S donor, for 11 weeks. Lung tissue H2S was evaluated by sulfide-sensitive electrode. Apoptosis of PASMCs were detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labelling （TUNEL）. Expressions of Fas, bcl-2 and caspase-3 in the PASMCs were analyzed with immunochemical staining. Results Four weeks after the shunting operation, the apoptosis of PASMCs and expression of Fas and caspase-3 were significantly decreased （P 〈0.01）, but expression of bcl-2 increased significantly （P 〈0.01）. PPG administration further inhibited the apoptosis of PASMCs, downregulated the expression of Fas and caspase-3 （P 〈0.01）, but increased the expression of bcl-2 （P 〈0.01）. After 11 weeks of shunting operation, the apoptosis of PASMCs and expression of Fas and caspase-3 were significantly decreased （P 〈0.01）, but expression of bcl-2 increased obviously （P 〈0.01）. NariS administration significantly increased the apoptosis of PASMCs, upregulated the expression of Fas and caspase-3, but inhibited the expression of bcl-2. Conclusions H2S induces the apoptosis of PASMCs in the development o

关键词： high pulmonary blood flow pulmonary hypertension hydrogen sulfide apoptosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Biological removal of air loaded with a hydrogen sulfide and ammonia mixture

引用

Journal of Environmental Sciences 2004年第4期16卷 656-661页

作者： CHENYing-xu YINJun FANGShi DepartmentofEnvironmentalEngineering ZhejiangUniversityHangzhou310029China

The nuisance impact of air pollutant emissions from wastewater pumping stations is a major issue of concern to China. hydrogen sulfide and ammonia are commonly the primary odor and are important targets for removal. An alternative control technology, biofiltration, was studied. The aim of this study is to investigate the potential of unit systems packed with compost in terms of ammonia and hydrogen sulfide emissions treatment, and to establish optimal operating conditions for a full-scale conceptual design. The laboratory scale biofilter packed with compost was continuously supplied with hydrogen sulfide and ammonia gas mixtures. A volumetric load of less than 150 gH 2S/(m3·d) and 230 gNH 3/(m3·d) was applied for about fifteen weeks. hydrogen sulfide and ammonia elimination occurred in the biofilter simultaneously. The removal efficiency, removal capacity and removal kinetics in the biofilter were studied. The hydrogen sulfide removal efficiency reached was very high above 99%, and ammonia removal efficiency was about 80%. hydrogen sulfide was oxidized into sulphate. The ammonia oxidation products were nitrite and nitrate. Ammonia in the biofilter was mainly removed by adsorption onto the carrier material and by absorption into the water fraction of the carrier material. High percentages of hydrogen sulfide or ammonia were oxidized in the first section of the column. Through kinetics analysis, the presence of ammonia did not hinder the hydrogen sulfide removal. According to the relationship between pressure drop and gas velocity for the biofilter and Reynolds number, non-Darcy flow can be assumed to represent the flow in the medium.

关键词： biological removal hydrogen sulfide ammonia mixture

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：