检索结果-南通市图书馆

中国神经科学学会第十届全国学术会议

作者： Xiao-Wen MENG You-Lang ZHOU Jing-De TAO Hong-Hong ZHANG Shufen HU Guang-Yin XU Institute of Neuroscience Suzhou Key Laboratory of Pain Research and TherapyDepartment of NeurobiologySoochow University

Background hydrogen sulfide(HS),an endogenous gaseotransmitter/modulator,is becoming appreciated that it may be involved in a wide variety of processes including inflammation and nociception.However,the role for HS in nociceptive processing in trigeminal ganglion(TG) neuron remains largely unknown.The aim of this study was to investigate whether endogenous HS synthesizing enzyme cystathionine β-synthetase(CBS) plays a role in inflammatory pain in temporomandibular joint(TMJ) inflammation.Methods TMJ inflammatory pain was induced by TMJ injection of complete Freund’s adjuvant(CFA) in adult rats.Von Frey filaments were used to examine pain behavioral responses in rats following injection of CFA or normal saline(NS).Immunohistochemical analysis was employed to determine whether CBS was co-expressed in P2X3-positive TG neurons.Western blot analysis was carried out to measure protein expression.Whole cell patch clamp recordings were employed on acutely isolated TG neurons from rats 2 days after CFA or NS injection.Results(1) Two days after injection of CFA into TMJ capsule,escape threshold for mechanical stimulation in TMJ-inflamed rats was remarkably lower than that in control rats(P<0.05).(2) In rat TG neurons,CBS was co-expressed in P2X3-positive TG neurons.(3) CBS expression in TGs in rats with TMJ inflammation was greatly enhanced(P<0.05).CFA injection also markedly upregulated P2X3 receptor expression(P<0.05).(4) The excitability of TG neurons from rats with TMJ inflammation was increased,mainly featured as decreased rhobase and increased repetitive discharge frequency.In addition,ATP-evoked current density was also remarkably increased in CFA injected rats when compared with that in control rats(P<0.05).(5) The reduced escape threshold was partially reversed by injection of O-(Carboxymethyl) hydroxylamine hemihydrochloride(AOAA),an inhibitor for CBS,in a dose-dependent manner.(6) Application of AOAA in TMJ reversed enhanced neural hyperexcitability of TG neurons,and also reversed mean peak current density of AT

关键词： temporomandibular joint inflammation hydrogen sulfide cystathionine-β-synthase P2X3 recept trigeminal ganglion excitability ATP current

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

气体信号分子硫化氢和慢性内脏痛

气体信号分子硫化氢和慢性内脏痛

引用

中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：徐广银苏州大学神经科学研究所疼痛转化医学实验室

hydrogen sulfide（H2S）is a well-known toxic gas that has the smell of rotten eggs.This pungent gas is synthesized from the amino acids:cysteine and homocysteine by four enzymes:cystathionine-β-synthase（CBS）,cystathionine-γ-lyase（CSE）and mercaptopuryvate sulphurtransferase（3-MST）along with cysteine aminotransferase（CAT）.In addition,two classes of bacteria in gastrointestinal（GI）track have been identified,that is,sulfate-and sulfur-reducing and sulfur-oxidizing bacteria,both contributing to the balance of the H2S level.The breakdown of proteins and peptides by colonic microorganisms yields a great diversity of end products including H2S.H2S exerts its effects on multiple physiological processes important under both normal and pathophysiological conditions.These functions include neuromodulation,regulation of blood pressure and cardiac function,inflammation,cellular energetics and apoptosis.hydrogen sulfide,like carbon monoxide or nitric oxide,is now becoming appreciated as a signaling molecule in many biological systems,including the GI track.However,the pathophysiological role and thetherapeutic potential of H2S signaling in chronic visceral pain states remain largely unknown.Our laboratory has spent a considerable amount of time to investigate the role and signaling pathways of H2S in different chronic visceral pain models,including irritable bowel syndrome（IBS）and functional dyspepsia.We demonstrated that H2S played pro-inflammatory functions,as well as pro-nociceptive roles in chronic visceral hypersensitivity.In particular,CBS-H2S signaling modulated a great diversity of ion channels/receptors in primary sensory neurons innervating GI organs.In addition,epigenetic regulations,particularly the demethylation of CBS gene promoter,are involved in upregulation of CBS expression under pathophysiological conditions.These studies identified CBS as a promising biomarker and a potential target for treatment of chronic visceral pain in functional GI disorders such as IBS.

关键词： Functional gastrointestinal disorders Dorsal root ganglion Chronic visceral pain hydrogen sulfide Ion channels

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Cystathionine β-synthase and cystathionine γ-lyase regulate VEGF and s Flt-1 production in human placenta and contribute to the pathogenesis of preeclampsia

Cystathionine β-synthase and cystathionine γ-lyase regulat...

引用

中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：胡天晓李苑何平唐志萍高路王钢孙倩倩朱晓燕古航倪鑫第二军医大学生理学教研室第二军医大学附属长海医院妇产科

Imbalance release of proangiogenic and antiangiogenic factor from placenta causes maternal endothelial dysfunction,the main manifestation of preeclampsia（PE）.Recently,hydrogen sulfide（H2S）has been shown to have a pro-angiogenic effect and human placenta is able to produce H2S.We therefore determined whether H2S is involved in the regulation of proangiogenic and antiangiogenic factors in placenta.H2S synthesis enzymes cystathionineβ-synthase（CBS）and cystathionineγ-lyase（CSE）expression was significantly downregulated in PE placentas.Both CSE and CBS positively correlated with the level of agiogenic factor vascular endothelial growth factor A（VEGFA）whereas inversely correlated to antiangiogenic factor soluble fms-like tyrosine kinase 1（s Flt-1）in placentas.H2S donor Na HS and precursor L-cysteine increased VEGFA whereas decreased s Flt-1 production in cultured placental explants as well as placental trophoblasts.The effects of H2S precursor L-cysteine could be reversed by si RNA of CBS and CSE.The selective KATP inhibitor glybenclamide or specific PI3K,ERK and P38 inhibitors did not block the effects of H2S on VEGFA and s Flt-1.H2S affected the stability of VEGFA and s Flt-1 m RNA in placental cells.Treatment of cells with Na HS or L-cysteine decreased the expression of mi R200c,mi R 20a and mi R20b which target VEGFA whereas increased the expression of mi R-133b which target s Flt-1.The levels of mi R-200c,mi R 20a and mi R20b were upregulated whereasthe level of mi R-133b was downregulated in PE placentas.The expression of mi R133b positively correlated to CBS and CSE level whereas mi R20a level inversely correlated to CBS and CSE expression in placentas.Our results suggest that H2S is an important factor that maintains the balance of proangiogenic factor VEGFA and antiangiogenic factor s Flt-1 in placenta.Reduced H2S generation in placenta contributes to the pathogenesis of PE.

关键词： hydrogen sulfide cystathionine β-synthase cystathionine γ-lyase regulate vascular endothelial growth factor soluble fms-like tyrosine kinase 1

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Promoted interaction of nuclear factor-kappa B with demethylated cystathionine-beta-synthetase gene contributes to gastric hypersensitivity in diabetic rats

Promoted interaction of nuclear factor-kappa B with demethyl...

引用

中国神经科学学会第十届全国学术会议

作者： Hong-Hong ZHANG Ji HU You-Lang ZHOU Shu-Fen HU Yong-Meng WANG Wei CHEN Ying XIAO Li-Yen Mae HUANG Xing-Hong JIANG Guang-Yin XU Division of Endocrinology the Second Affiliated HospitalSoochow University Institute of Neuroscience Suzhou Key Laboratory of Pain Research and TherapyDepartment of NeurobiologySoochow University Department of Neuroscience and Cell Biology University of Texas Medical Branch

Objective The study was designed to determine the role for nuclear factor-kappaB(NF-kappaB) and hydrogen sulfide(HS) signaling pathways in diabetic gastric hypersensitivity.Methods Diabetes were induced by intraperitoneal(i.p.) injection of streptozotocin(STZ,65 mg/kg) in adult female rats.Behavior responses to graded gastric balloon distention will be employed on normal and diabetic rats.Patch clamp recordings were performed in vitro on single gastric-specific DRG neurons acutely isolated from these rats labeled with dye DiI.Expression of HS producing enzyme Cystathionine- beta-synthetase(CBS) and DNA methyl-transferase(DNMT) were measured with western blotting and real-time PCR analysis.DNA methylation status were determined by methylation specific PCR and bisulfite sequencing.Recruitment of cbs gene pairs NF-kappaB was analyzed with chromatin immunoprecipitation assays.Results(1) Diabetic rats were more sensitive to graded gastric balloon distention 4 weeks after STZ treatment(vs.controls,P<0.01).I.p.injection of CBS inhibitor(Amino-oxyacetic acid,AOAA) could attenuate diabetic gastric hyperalgesia.(2) The excitability of gastric-specific DRG neurons from diabetic rats was increased.One week after i.***.injection of AOAA,the neuronal excitability was decreased.In addition,NaHS(donor of HS) increased the excitability of the gastric-specific DRG neurons of normal rats.(3) CBS and CBS mRNA expression of gastric DRGs in diabetic rats was greatly enhanced(P<0.05).Injection with NF-kappaB inhibitor(Pyrrolidine dithiocarbamate,PDTC) for 1week significantly reduced upregulation of CBS expression.(4) cbs gene promoter region of gastric DRGs contained recognition sequence region of NF-kappaB and was significantly demethylated in diabetic rats(vs.controls,P<0.05).Chromatin immunoprecipitation assay showed that intracellular NF-kappaB could bind specifically to cbs gene promoter region in diabetic rats.(5) DNMT mRNA expression of gastric-specific DRGs in diabetic rats was greatly decreased(P<0.05).Conclusions Our findings s

关键词： diabetes gastric hypersensitivity hydrogen sulfide nuclear factor-kappaB DNA methylation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The cardiovascular effects of central hydrogen sulphide are related to KATP channels opening mechanism

The cardiovascular effects of central Hydrogen sulphide are ...

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Yan LU~(1,*),Wen-Qing LIU~2,Xiao-Yun LI~1,Gong-Min GAO~1 1 Department of Clinical Laboratory,San Ai Tang Hospital,Lanzhou 730030,China 2 Department of General Surgery,First Hospital of Lanzhou University,730000,LanZhou,China

objective Although hydrogen sulphide(HS),an endogenous "gasotransmitter",existing with a high concetration in the central nervous system,the central cardiovascular effects of endogenous HS are not fully determined.The present study was designed to comparatively investigate the central cardiovascular effects and mechanism of endogenous HS in anesthetized rats.Methods the central cardiovascular functions of endogenous HS were determined by intracerebroventricular(icv) apphcation of NaHS(0.1 mmol),the donor of HS,or CBS activator s-adenosyl-L-methionine (SAM,0.05 mmol) into lateral cerebral ventricle(LCV) in anesthetized rats.CBS inhibitor hydroxylamine(HA,10 mmol) or K blocker glibenclamide(1 g/L) were prior applied into LCV to observe the central NaHS-induced cardiovascular responses after CBS was inhibited or K channels was blocked.Results Icv injection of NaHS(0.1 mmol) produced a significant decrease in BP(from 93±11 to 67±8 mmHg,P<0.05) and HR(from 450±14 to 370±38 bpm,P<0.05) compared to control.Icv injection of SAM(0.05μmol) also produced a significant hypotension(from 94±4 to 71±10 mmHg,P<0.05) and bradycardia(from 444±35 to 385±64 bmp,P<0.05),which was similar to those of icv injection of NaHS.Furthermore, the hypotension and bradycardia induced by icv injection of NaHS could effectively be abolished by pretreatment with glibenclamide but not with HA.Conclusion The present study suggests that central HS produces hypotension and bradycardia,at least partly,by the K channel opening mechanism.

关键词： rat hydrogen sulfide blood pressure heart rate

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Epigenetic regulations of chronic visceral hypersensitivity in irritable bowel syndrome

Epigenetic regulations of chronic visceral hypersensitivity ...

引用

中国神经科学学会第十届全国学术会议

作者： Liyan Zhu Ruobing Qu Shufen Hu Ying Xiao Guang-Yin Xu Institute of Neuroscience Soochow University

Irritable bowel syndrome(IBS),characterized by persistent or recurrent abdominal pain with altered bowel movement in the absence of a demonstrable pathology,is one of the most common functional gastrointestinal disorders.The courses of IBS remain unkown and treatment options for chronic visceral pain are limited.hydrogen sulfide(HS),a third gaseous modulator/mediator,has become recognized as an important endogenous molecular.HS is synthesized from L-cysteine primarily via two endogenous enzymes:cystathionine-β-synthetase(CBS) and cystathionine-γ-lyase,and has been reported to be involved in nociceptive signaling and inflammation.However,the molecular and epigenetic mechanisms of CBS-HS signaling in visceral nociceptive processing are not fully understood.Here we showed that IBS-like chronic visceral hyperalgesia induced by colonic injection of 0.5%acetic acid in 10-day-old rats significantly upregulated expression of CBS at both protein and mRNA levels in rat dorsal root ganglia(DRG).Intraperitoneal administration of CBS antagonist aminooxyacetic acid(AOAA) attenuated visceral hyperalgesia in a dose-dependent manner in rats with visceral hyperalgesia.In addition,application of AOAA reversed the enhanced excitability,reduced the upregulation of voltage-gated sodium channels Na1.7 and Na1.8 in DRGs and decreased total sodium current of DRG neurons in rats with visceral hyperalgesia.We also analyzed cbs gene promoter region by bioinformatics tools and found two CpG sites.Methylation specific PCR demonstrated that promoter region of cbs gene was less methylated in DRG samples from rats with visceral hyperalgesia than that from controls.Neonatal colonic infusion upregulated the expression of thymine DNA glycosylase while expression of DNA methylation transferases was not altered significantly.Our results suggest that epigenetic regulation of CBS expression may contribute to the visceral hyperalgesia,thus identifying a potential therapeutic target for the treatment of chronic visceral pain in patients with irritable bo

关键词： irritable bowel syndrome visceral hypersensitivity dorsal root ganglion hydrogen sulfide epigenetics

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

CBS and CSE are critical for maintenance of mitochondrial function and glucocorticoid production in adrenal cortex

CBS and CSE are critical for maintenance of mitochondrial fu...

引用

中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：倪鑫王长楠朱晓燕第二军医大学基础部生理学教研室

Aims:Reversible adrenal insufficiency has frequently been diagnosed in critically ill patients with sepsis,however the mechanisms underlying down-regulation of GCs production during sepsis remain largely unknown.Mitochondria play a central role in adrenocortical steroidogenesis.Recently,hydrogen sulfide（H2S）,a gaseous transmitter endogenously produced by cystathionine-β-synthase（CBS）andcystathionine-γ-lyase（CSE）,has been found to improve mitochondrial function.The present study aimed at examining whether CBS and CSE are expressed in adrenal glands,and investigated the role of these enzymes in the maintenance of mitochondrial function and the production of glucocorticoids in adrenocortical cells.Results:Both CBS and CSE are present in murine adrenocortical cells and account for H2S generation in adrenal glands.Using a combination of both in vivo and in vitro approaches,we demonstrated that either CBS/CSE inhibitors or small interfering RNAs led to mitochondrial oxidative stress and dysfunction,which meanwhile resulted in blunted corticosterone responses to adrenocorticotropic hormone（ACTH）.These effects were significantly attenuated by the treatment of H2S donor GYY4137.Lipopolysaccharide（LPS）also caused mitochondrial damage,thereby resulting in adrenal insufficiency.Both mitochondrial superoxide dismutase 2 mimetic Mn TBAP and mitochondrial superoxide scavenger mito-TEMPO which were used to block mitochondria superoxide production could reverse LPS-induced mitochondrial oxidative stress and dysfunction and prevented LPS-induced corticosterone hyporesponsiveness to ACTH.Moreover,LPS inhibited CBS/CSE expression and H2S production in adrenal glands and primary adrenocortical cells,while H2S donor GYY4137 protected against LPS-induced mitochondrial damage and hyporesponsiveness to ACTH.Conclusions:Endogenous H2S is critical for maintaining mitochondrial function and glucocorticoid production in adrenal cortex.LPS inhibit the expression of H2S-forming enzyme and production of H2S in mouse adrenal glands.This may be o

关键词： hydrogen sulfide CBS CSE mitochondria adrenal gland

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Carbon monoxide: impact on remethylation/transsulfuration metabolism and its pathophysiologic implications

引用

世界最新医学信息文摘 2015年第5期 245-245页

作者： Takako Hishiki Takehiro Yamamoto Takayuki Morikawa Akiko Kubo Mayumi Kajimura Makoto Suematsu

Carbon monoxide(CO) is a gaseous product generated by heme oxygenase(HO), which oxidatively degrades heme. While the stress-inducible HO-1 has well been recognized as an anti-oxidative defense mechanism under stress conditions, recent studies suggest that cancer cells utilize the reaction for their survival. HO-2, the constitutive isozyme, also plays protective roles as a tonic regulator for neurovascular function. Although protective roles of the enzyme reaction and CO have extensively been studied, little information is available on the molecular mechanisms by which the gas exerts its biological actions. Recent studies using metabolomics revealed that CO inhibits cystathionine β-synthase(CBS), which generates H2 S, another gaseous mediator. The CO-dependent CBS inhibition may impact on the remethylation cycle and related metabolic pathways including the methionine salvage pathway and polyamine synthesis. This review focuses on the gas-responsive regulation of metabolic systems, particularly the remethylation and transsulfuration pathways, and their putative implications for cancer and ischemic diseases.

关键词： Heme oxygenase hydrogen sulfide Cystathionine β-synthase Methylation Glutathione Cancer Methionine salvage pathway Epigenetics Metabolic systems Gas biology

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：