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Molecular Identification of hyalomma anatolicum,hyalomma asiaticum and hyalomma detritum

Animal Husbandry and Feed Science 2014年第3期6卷 118-122页

作者： Lv Jizhou Wu Shaoqiang Zhang Yongning Wang Zhenbao Feng Chunyan Wang Caixia Deng Junhua Yuan Xiangfen Lin Xiangmei Institute of Animal Quarantine Chinese Academy of Inspection and Quarantine Yili Entry-exit Inspection and Quarantine Bureau

hyalomma anatolicum, hyalomma asiaticum and hyalomma detritum are wide-spread tick vectors in China. They could transmit a great variety of serious animal and human pathogens, which are great threats to the health of human beings and the safety of stockbreeding. Most of them are distributed in Xinjiang and Inner Mongolia, and they share similar morphologies. This study is to establish a method for identifying H. anatolicum, H. asiaticum and H. detritum with molecular markers and to revealing the phylogenetic relationship of these ticks. Ticks were collected from domestic animals in Xinjiang and Inner Mongolia autonomous regions and classified by morphological characters. 16S rRNA and mitachondrial cytochrome oxidase subunit I gone （CO1） of ticks were amplified by PCR and subsequently sequenced. Phylogenetic trees were constructed by MEGA 5.0 and Mrbayes 3.2. On the 16S rRNA-based phylogenetic tree, H. anatolicum and H. aisaticum were clustered together with their respective classes. H. detritum was clustered with their respective class and the H. anatolicum, H. asiaticum formed distinct branches on the phylogenetic trees based on the COL The method based on morphology that combined with molecular 16S rRNA and COl seemed a simple and accurate method for species identification of H. anatolicum, H. asiaticum and H. detritum. n

关键词： Cytochrome oxidase subunit I gone (COI) 16S rRNA hyalomma anatolicum hyalomma asiaticum hyalomma detritum Molecular identificatio

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