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Identification of differentially expressed genes in human uterine leiomyomas using differential display

Cell Research 2002年第1期12卷 39-45页

作者： BIN LI, MEI SUN, BIN HE, JIN YU, You DUAN ZHANG, YONG LIAN ZHANG, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Science, Chinese Academy of Sciences, 820, Yue- Yang Road, Shanghai, 1State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Science Chinese Academy of Sciences 320 Yue-Yang Road Shanghai 200031 China 1State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Science Chinese Academy of Sciences 320 Yue-Yang Road Shanghai 200031 China 1State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Science Chinese Academy of Sciences 320 Yue-Yang Road Shanghai 200031 China 2OB/GYN Hospital Shanghai (First) Medical University 419 Fang-Xie Road Shanghai China 1State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Science Chinese Academy of Sciences 320 Yue-Yang Road Shanghai 200031 China 1State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Science Chinese Academy of Sciences 320 Yue-Yang Road Shanghai 200031 China

In searching of differentially expressed genes in human uterine leiomyomas, differential display was used with twelve pairs of primers to compare human uterine leiomyomas with matched myometrium. False positives were eliminated by reverse Northern analysis. Positives were confirmed by Northern blot analysis. RESULTS: [1] Four of 69 cDNA fragments (3 up-regulated named L1, L2 and L3 and 1 down-regulated named Mi in leiomyoma) were confirmed by Northern analysis. [2] Sequence comparison and Northern analysis proved that Li is exactly the human ribosomal protein Si9. [3] It was present ubiquitously in i3 tissues tested but in various levels and even in different size. [4] Li was highly expressed in parotidean cystadenocarcinoma, pancreatic cancer and breast cancer examined. [5] No mutations have been found in human uterine leiomyomas (n=6). CONCLUSIONS: hRPSi9 overexpression might be a universal signal in rapid cell growth tissues.

关键词： human uterine leiomyomas differentially expressed gene differential display (DD) human S19 ribosomal protein (hRPS19) up-regulated gene in human leiomyomas.

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