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huangqin decoction Attenuates DSS-Induced Mucosal Damage and Promotes Epithelial Repair via Inhibiting TNF-α-Induced NF-κB Activation

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Chinese Journal of Integrative Medicine 2022年第2期28卷 124-129页

作者： GU Li-mei LI Hui XIA Jun-quan PAN Cheng-yu GU Chao TIAN Yao-zhou Gastroenterology Department Affiliated Hospital of Integrated Traditional Chinese and Western MedicineNanjing University of Chinese MedicineNanjing(210028)China Gastroenterology Department Jiangsu Province Hospital on Integration of Chinese and Western MedicineNanjing(210028)China Jiangsu Province Academy of Traditional Chinese Medicine Nanjing(210028)China

Objective:To investigate the protective effect of Chinese herbal formula huangqin decoction(HQD)on ulcerative colitis mouse model induced by dextran sulphate sodium(DSS)and human intestinal epithelial cell injury induced by tumour necrosis factor-α(TNF-α).Methods:In vivo,30 male C57BL/6 mice were divided into 5 groups using a random number table(n=6 per group),including control,DSS,5-aminosalicylic acid(5-ASA),HQD low-(HQD-L)and high-dose(HQD-H)*** colitis mouse model was established by 3%(w/v)DSS water for 5 ***,mice in the HQD-L,HQD-H and 5-ASA groups were administrated with 100,200 mg/kg HQD or 100 mg/kg 5-ASA,respectively,once daily by *** 9 days of administration,the body weight,disease activity index(DAI)score and colon length of mice were measured,the pathological changes of colons were analyzed by hematoxylin-eosin staining(HE)staining,and the levels of serum interleukin(IL)-6,IL-1βand TNF-αwere measured by enzyme linked immunosorbent *** vitro,the human colon epithelial normal cells(FHC cells)were exposed to HQD(0.6 mg/mL)for 12 h and then treated with TNF-α(10 ng/mL)for 24 *** tight junction(TJ)protein expression levels of Claudin-4 and Occludin,and the protein phosphorylation levels of p65 and inhibitor of nuclear factor kappaB(NF-κB)-α(IκBα)were measured by Western ***:In vivo,compared with the DSS group,HQD-H treatment attenuated the weight loss and reduced DAI score of mice on the 8th day(P<0.05).Moreover,HQD-H treatment ameliorated the colon shortening in the DSS-induced colitis mice(P<0.05).HE staining showed HQD attenuated the pathological changes of colitis mice,and the histological scores of HQD-H and 5-ASA groups were significantly decreased compared with the DSS group(P<0.05).Meanwhile,HQD-H and 5-ASA significantly decreased the serum IL-1β,IL-6 and TNF-αlevels of mice(P<0.05).In vitro experiments showed that HQD up-regulated Occludin and Claudin-4 protein expressions and inhibited p-p65 and p-IκBαlevel

关键词： huangqin decoction ulcerative colitis tumour necrosis factor-α nuclear factor kappaB pathway tight conjunction protein Chinese medicine

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huangqin decoction Delays Progress of Colitis-Associated Carcinogenesis by Regulating Nrf2/HO-1 Antioxidant Signal Pathwayin Mice

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Chinese Journal of Integrative Medicine 2024年第2期30卷 135-142页

作者： GU Li-mei LI He-zhong GAO Lei LI Hui WEI Lan-fu PAN Cheng-yu WU Ke-xuan TIAN Yao-zhou Department of Gastrointestinal Endoscopy Affiliated Hospital of Nanjing University of Chinese MedicineNanjing(210029)China Department of Gastroenterology Affiliated Hospital of Integrated Traditional Chinese and Western MedicineNanjing University of Chinese MedicineNanjing(210028)China School of Integrated Chinese and Western Medicine Nanjing University of Chinese MedicineNanjing(210023)China

Objective:To investigate the effect of huangqin decoction(HQD)on nuclear factor erythroid 2 related-factor 2(Nrf2)/heme oxygenase(HO-1)signaling pathway by inducing the colitis-associated carcinogenesis(CAC)model mice with azoxymethane(AOM)/dextran sodium sulfate(DSS).Methods:The chemical components of HQD were analyzed by liquid chromatography-quadrupole-time-of-flight mass spectrometry(LC-Q-TOF-MS/MS)to determine the molecular constituents of *** 48 C57BL/6J mice were randomly divided into 6 groups by a random number table,including control,model(AOM/DSS),mesalazine(MS),low-,medium-,and high-dose HQD(HQD-L,HQD-M,and HQD-H)groups,8 mice in each *** for the control group,the mice in the other groups were intraperitoneally injected with AOM(10 mg/kg)and administrated with 2.5%DSS orally for 1 week every two weeks(totally 3 rounds of DSS)to construct a colitis-associated carcinogenesis mouse *** mice in the HQD-L,HQD-M and HQD-H groups were given HQD by gavage at doses of 2.925,5.85,and 11.7 g/kg,respectively;the mice in the MS group was given a suspension of MS at a dose of 0.043 g/kg(totally 11 weeks).The serum levels of malondialdehyde(MDA)and superoxide dismutase(SOD)were measured by enzyme-linked immunosorbent *** mRNA and protein expression levels of Nrf2,HO-1,and inhibitory KELCH like ECH-related protein 1(Keap1)in colon tissue were detected by quantitative real-time PCR,immunohistochemistry,and Westem blot,***:LC-Q-TOF-MS/MS analysis revealed that the chemical constituents of HQD include baicalin,paeoniflorin,and glycyrrhizic *** to the control group,significantly higher MDA levels and lower SOD levels were observed in the model group(P<0.05),whereas the expressions of Nrf2 and HO-1 were significantly decreased,and the expression of Keap1 increased(P<0.01).Compared with the model group,serum MDA level was decreased and SOD level was increased in the HQD-M,HQD-H and MS groups(P<0.05).Higher expressions of

关键词： huangqin decoction colitis-associated carcinogenesis nuclear factor erythroid 2 related-factor 2/heme oxygenase signaling pathway oxidative stress Chinese medicine

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huangqin decoction alleviates lipid metabolism disorders and insulin resistance in nonalcoholic fatty liver disease by triggering Sirt1/NF-κB pathway

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World Journal of Gastroenterology 2023年第31期29卷 4744-4762页

作者： Bao-Fei Yan Lan-Fen Pan Yi-Fang Quan Qian Sha Jing-Zheng Zhang Yi-Feng Zhang Li-Bing Zhou Xi-Long Qian Xiao-Mei Gu Feng-Tao Li Ting Wang Jia Liu Xian Zheng College of Pharmacy Jiangsu Health Vocational CollegeNanjing 211800Jiangsu ProvinceChina Department of Pathology Affiliated Kunshan Hospital of Jiangsu UniversityKunshan 215300Jiangsu ProvinceChina Department of Education and Science The First People's Hospital of TaicangKunshan 215400Jiangsu ProvinceChina Department of Pharmacy Affiliated Hospital of Xuzhou Medical UniversityXuzhou 221006Jiangsu ProvinceChina School of Pharmacy Nantong UniversityNantong 226019Jiangsu ProvinceChina Department of Pharmacy Affiliated Kunshan Hospital of Jiangsu UniversityKunshan 215300Jiangsu ProvinceChina School of Pharmacy Nanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina

BACKGROUND Nonalcoholic fatty liver disease(NAFLD)is a clinicopathological entity characterized by intrahepatic ectopic *** a consequence of increased consumption of high-calorie diet and adoption of a sedentary lifestyle,the incidence of NAFLD has surpassed that of viral hepatitis,making it the most common cause of chronic liver disease *** decoction(HQD),a Chinese medicinal formulation that has been used clinically for thousands of years,has beneficial outcomes in patients with liver diseases,including ***,the role and mechanism of action of HQD in lipid metabolism disorders and insulin resistance in NAFLD remain poorly *** To evaluate the ameliorative effects of HQD in NAFLD,with a focus on lipid metabolism and insulin resistance,and to elucidate the underlying mechanism of *** High-fat diet-induced NAFLD rats and palmitic acid(PA)-stimulated HepG2 cells were used to investigate the effects of HQD and identify its potential mechanism of *** in HQD were analyzed by highperformance liquid chromatography(HPLC)to identify the key *** Ten primary chemical components of HQD were identified by HPLC *** vivo,HQD effectively prevented rats from gaining body and liver weight,improved the liver index,ameliorated hepatic histological aberrations,decreased transaminase and lipid profile disorders,and reduced the levels of pro-inflammatory factors and insulin *** vitro studies revealed that HQD effectively alleviated PA-induced lipid accumulation,inflammation,and insulin resistance in HepG2 ***-depth investigation revealed that HQD triggers Sirt1/NF-κB pathwaymodulated lipogenesis and inflammation,contributing to its beneficial actions,which was further corroborated by the addition of the Sirt1 antagonist EX-527 that compromised the favorable effects of *** In summary,our study confirmed that HQD mitigates lipid metabolism disorders and insulin resistance i

关键词： Nonalcoholic fatty liver disease huangqin decoction Lipid metabolism disorders Insulin resistance Sirt1/NF-κB pathway

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Network pharmacology and molecular docking-based analyses to predict the potential mechanism of huangqin decoction in treating colorectal cancer

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World Journal of Clinical Cases 2023年第19期11卷 4553-4566页

作者： Ying-Jie Li Dong-Xin Tang Hong-Ting Yan Bing Yang Zhu Yang Feng-Xi Long Guizhou University of Traditional Chinese Medicine Guizhou University of Traditional Chinese MedicineGuiyang 550005Guizhou ProvinceChina Digestive Department The First Affiliated Hospital of Guizhou University of Traditional Chinese MedicineGuiyang 550001Guizhou ProvinceChina Guizhou University of Traditional Chinese Medicine Guizhou University of Traditional Chinese MedicineGuiyang 550001Guizhou ProvinceChina Guizhou University of Traditional Chinese Medicine Guizhou University of Traditional Chinese MedicineGui Yang 550001Guizhou ProvinceChina

BACKGROUND To analyze the potential action mechanism of huangqin decoction(HQD)in colorectal cancer(CRC)treatment on the basis of network pharmacology and molecular *** To investigate the molecular mechanisms of HQD for CRC treatment by using network pharmacology and molecular *** All HQD active ingredients were searched using the Systematic Pharmacology and Traditional Chinese Medicine Systems Pharmacology databases and the Bioinformatics Analysis Tool for Molecular Mechanisms in traditional Chinese ***,the targets of the active ingredients were *** abbreviations of protein targets were obtained from the UniProt database.A“drug–compound–target”network was constructed to screen for some main active *** targets related to the therapeutic effect of CRC were obtained from the GeneCards,DisGeNET,Therapeutic Target Database,and Online Mendelian Inheritance in Man *** intersection of targets of Chinese herbs and CRC was taken.A Venn diagram was drawn to construct the intersection target interactions network by referring to the STRING *** analysis of the protein interaction network was performed using Cytoscape 3.7.2 software to screen the core HQD targets for *** core targets were imported into the DAVID 6.8 analysis website for gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses and ***,molecular docking was performed using AutoDockTool and PyMOL for *** In total,280 potential drug-active ingredients were present in HQD,including 1474 targets of the drug-active *** main active ingredients identified were betulin,tetrahydropalmatine,and *** total,10249 CRC-related targets and 1014 drug-disease intersecting targets were identified,including 28 core targets of action such as Jun proto-oncogene,AP-1 transcription factor subunit,signal transducer and activator of transcription 3,tumor protein p53,vascular endothelia

关键词： huangqin decoction Colorectal cancer Network pharmacology

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A Network Pharmacology Approach to Investigate the Mechanisms of huangqin decoction in the Treatment of Irinotecan-Induced Gastrointestinal Toxicity

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Asian Toxicology Research 2020年第1期2卷 8-21页

作者： Yu Wang Qing Xu Zheng-Quan Feng The Second Clinical Medical College of Zhengjiang Chinese Medical University Zhengjiang310053China Zhengjiang Provincial Tongde Hospital Zhengjiang 310053China

Objective:To find out the potential mechanisms of huangqin decoction in the treatment of irinotecan-induced gastrointestinal ***:A network pharmacology approach was used to analyze the active compounds,drug targets and interacting pathways of huangqin decoction in treating irinotecan-induced gastrointestinal *** compounds and predicted targets of huangqin decoction were screened from TCMSP,and the disease targets were obtained from *** therapeutic mechanisms of action of the huangqin decoction were analyzed by gene ontology(GO)enrichment,Kyoto encyclopedia of genes and genomes pathway(KEGG)enrichment ***:The results show that 161compounds and 143 targets were obtained in this *** targets were further mapped to 216 GO biological process terms and 30 remarkably *** compounds,targets,and pathways were used to construct a compound-target *** results indicated that huangqin decoction may treat the irinotecan-induced gastrointestinal toxicity mainly from intervening in the mucosal inflammation,cell apoptosis process,and cell ***:This study confirmed that the active components of huangqin decoction play an important role in the treatment of irinotecan-related gastrointestinal toxicity through multi-target and multi-pathway,which provides a new way for the pathogenesis of irinotecan-related gastrointestinal *** facilitates the modernization of herbal medicine for complex diseases in the future.

关键词： Network pharmacology huangqin decoction Irinotecan Gastrointestinal toxicity Pathogenesis

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