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Elevated axonal membrane permeability and its correlation with motor deficits in an animal model of multiple sclerosis

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Translational Neurodegeneration 2017年第1期6卷 29-36页

作者： Gary Leung Melissa Tully Jonathan Tang Shengxi Wu Riyi Shi Department of Basic Medical Sciences College of Veterinary MedicinePurdue UniversityWest LafayetteIN 47907USA Weldon School of Biomedical Engineering Purdue UniversityWest LafayetteIN 47907USA MSTP program Indiana University School of MedicineIndianapolisINUSA Department of Neurobiology Fourth Military Medical UniversityXi’anChina

Background:It is increasingly clear that in addition to myelin disruption,axonal degeneration may also represent a key pathology in multiple sclerosis(MS).Hence,elucidating the mechanisms of axonal degeneration may not only enhance our understanding of the overall MS pathology,but also elucidate additional therapeutic *** objective of this study is assess the degree of axonal membrane disruption and its significance in motor deficits in EAE ***:Experimental Autoimmune Encephalomyelitis was induced in mice by subcutaneous injection of myelin oligodendrocyte glycoprotein/complete Freud’s adjuvant emulsion,followed by two intraperitoneal injections of pertussis *** assessment was performed using a 5-point *** peroxidase Exclusion test was used to quantify the disruption of axonal *** glycol was prepared as a 30%(w/v)solution in phosphate buffered saline and injected ***:We have found evidence of axonal membrane disruption in EAE mice when symptoms peak and to a lesser degree,in the pre-symptomatic stage of EAE ***,polyethylene glycol(PEG),a known membrane fusogen,significantly reduces axonal membrane disruption in EAE *** PEG-mediated membrane repair was accompanied by significant amelioration of behavioral deficits,including a delay in the emergence of motor deficits,a delay of the emergence of peak symptom,and a reduction in the severity of peak ***:The current study is the first indication that axonal membrane disruption may be an important part of the pathology in EAE mice and may underlies behavioral *** study also presents the initial observation that PEG may be a therapeutic agent that can repair axolemma,arrest axonal degeneration and reduce motor deficits in EAE mice.

关键词： Multiple sclerosis EAE Axonal membrane damage Polyethylene glycol Acrolein horseradish peroxidase Membrane permeability Neurodegeneration

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Hydrogen peroxide biosensor based on electrodeposition of zinc oxide nanoflowers onto carbon nanotubes film electrode

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Chinese Chemical Letters 2008年第3期19卷 314-318页

作者： Hui Ping Bai Xu Xiao Lu Guang Ming Yang Yun Hui Yang Department of Chemistry and Chemical Engineering Yunnan Normal University Kunming 650092 China

A new amperometric biosensor for hydrogen peroxide was developed based on adsorption of horseradish peroxidase at the glassy carbon electrode modified with zinc oxide nanoflowers produced by electrodeposition onto multi-walled carbon nanotubes （MWNTs） film. The morphology of the MWNTs/nano-ZnO electrode has been investigated by scanning electron microscopy （SEM）, and the electrochemical performance of the electrode has also been studied by amperometric method. The resulting electrode offered an excellent detection for hydrogen peroxide at -0.11 V with a linear response range of 9.9×10^-7 to 2.9×10^-3 mol/L with a correlation coefficient of 0.991, and response time 〈5 s. The biosensor displays rapid response and expanded linear response range, and excellent stability.

关键词： Multi-walled carbon nanotubes Biosensor Electrodeposition horseradish peroxidase ZnO nanoflowers

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Nerve-pathways of acupoint Fengch'ih in rat by anterograde transport of HRP

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World Journal of Gastroenterology 2005年第20期11卷 3135-3138页

作者： Gang-MingXi Hua-QiaoWang Guo-HouHe Chao-FengHuang Qun-FangYuan Guo-YaoWei HuaLi Wen-WenLiu Hua-YanFan DepartmentofNeurology ShiyanRenminHospitalAffiliatedtoYunyangMedicalCollegeShiyan442000HubeiProvinceChina DepartmentofAnatomy&BrainResearch PreclinicalMedicineSchoolSunYat-SenUniversityGuangzhou510080GuangdongProvinceChina DepartmentofNeurology TaiheHospitalAffiliatedtoYunyangMedicalCollegeShiyan442000HubeiProvinceChina

AIM: To study the nervous-pathways of Fengch'ih acupuncture by means of anterograde transport of aqueous solution of horseradish peroxidase (HRP). METHODS: Fifty Wistar rats were randomly divided into 1, 2,3,4, and 5 d groups, and every group had 10 animals. HRP (30% aqueous solution) was injected into a Fengch'ih. Serial, transverse or capital, 40 urn sections of the cervical spinal ganglia, cervical and thoracic spinal cord segment and brain were cut on a cryotome. Sections were incubated for HRP histochemistry according to the tetramethylbenzidine (TMB). Part of the sections were counterstained with neutral red. RESULTS: After 1 d of survival times, many labeled cell bodies were found in 1-4 cervical spinal ganglia, anterior horn of 1-4 cervical spinal cord, ventromedial division of facial nucleus, accessory facial nucleus ipsilaterally. With increasing survival times, the intensity of labeled cells were slightly decreased. CONCLUSION: Fengch'ih may bring into full play its effect by correlation of posterior ear branch of facial nerve and anterior branch of 2-3 cervical nerve with 1-4 cervical the anterior horn of the spinal cord, ventromedial division of facial nucleus, accessory facial nucleus.

关键词： Point Fengch'ih Acupuncture horseradish peroxidase

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Determination of Minimum Enzymatic Decolorization Time of Reactive Dye Solution by Spectroscopic & Mathematical Approach

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光谱学与光谱分析 2015年第2期35卷 340-345页

作者： Mithat Celebi Zafer Omer Ozdemir Emre Eroglu Melda Altikatoglu Ibrahim Guney Yalova University Faculty of EngineeringPolymer Engineering Yildiz Technical University Faculty of Chemical and Metallurgical EngineeringDepartment of BioengineeringDavutpasa Campus Kirklareli University Technology FacultyDepartment of Energy Systems Engineering Kirklareli University Faculty of Arts and ScienceDepartment of Mathematics Yildiz Technical University Faculty of Arts and ScienceDepartment of ChemistryDavutpasa Campus Bitlis Eren University Faculty of Arts and ScienceDepartment of StatisticsBitlis Campus

Synthetic dyes are very important for textile dyeing,paper printing,color photography and petroleum *** methods of dye removal include biodegradation,precipitation,adsorption,chemical degradation,photo degradation,and chemical *** decolorization with enzymatic reaction is an important issue for several research field(chemistry,environment)In this study,minimum decolorization time of Remazol Brilliant Blue R dye with horseradish peroxidase enzyme was calculated using with mathematical equation depending on experimental *** decolorization was determined by monitoring the absorbance decrease at the specific maximum wavelength for *** experiments were carried out with different initial dye concentrations of Remazol Brilliant Blue R at 25 ℃ constant temperature for 30 *** development of the least squares estimators for a nonlinear model brings about complications not encountered in the case of the linear *** times for completely removal of dye were calculated according to *** was shown that mathematical equation was conformed exponential curve for dye degradation.

关键词： Decolorization horseradish peroxidase Mathematical modeling Reactive dye UV-visible spec-trophotometry

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Heme perbxidases are responsible for the dehydrogenation and oxidation metabolism of harmaline into harmine

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Chinese Journal of Natural Medicines 2022年第3期20卷 194-201页

作者： WANG You-Xu CAO Ning GUAN Hui-Da CHENG Xue-Mei WANG Chang-Hong Institute of Chinese Materia Medica Shanghai University of Traditional Chinese MedicineThe MOE Key Laboratory for Standardization of Chinese MedicinesShanghai Key Laboratory of Compound Chinese MedicinesShanghai 201203China

Harmaline and harmine areβ-carboline alkaloids with effective pharmacological *** can be transformed into harmine after oral ***,enzymes involved in the metabolic pathway remain *** this study,harmaline was incubated with rat liver microsomes(RLM),rat brain microsomes(RBM),blood,plasma,broken blood cells,and heme peroxidases including horseradish peroxidase(HRP),lactoperoxidase(LPO),and myeloperoxidase(MPO).The production of harmine was determined by a validated UPLC-ESI-MS/MS *** showed that heme peroxidases catalyzed the oxidative dehydrogenation of *** the reactions were in accordance with the Hill *** reaction was inhibited by ascorbic acid and excess H_(2)0_(2).The transformation of harmaline to harmine was confirmed after incubation with blood,plasma,and broken blood cells,rather than RLM and *** was incubated with blood,plasma,and broken cells liquid for 3 h,and the formation of harmine became *** indicated an integrated metabolic pathway of harmaline,which will lay foundation for the oxidation reaction of ***,the metabolic stability of harmaline in blood should not be ignored when the pharmacokinetics study of harmaline is carried out.

关键词： Harmaline Harmine Oxidative dehydrogenation Heme peroxidases Myeloperoxidase horseradish peroxidase Lactoperoxidase

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用免疫酶标组织化学观察大白鼠垂体LH细胞的分布与垂体门脉血管系统的支配关系

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生殖与避孕 1985年第3期 36-40页

作者：周寿康谢衷明陆文燕徐美红上海市计划生育科学研究所

应用辣根过氧化物酶标记抗体技术,对正常和阉割成年雌性大白鼠垂体LH细胞的分布进行了研究。结果发现在大白鼠垂体远侧部以下三个区域的LH细胞数量多、细胞大、分布集中:(1)头端部的腹部区;(2)头端部的背部区和邻近中间叶的两侧翼;(3)... 详细信息

应用辣根过氧化物酶标记抗体技术,对正常和阉割成年雌性大白鼠垂体LH细胞的分布进行了研究。结果发现在大白鼠垂体远侧部以下三个区域的LH细胞数量多、细胞大、分布集中:(1)头端部的腹部区;(2)头端部的背部区和邻近中间叶的两侧翼;(3)尾端部的背部区和邻近中间叶的两侧翼。阉割大白鼠在这三个区域内分布集中的特点比正常大白鼠更明显。作者认为LH细胞这种有规律的分布与垂体门脉血管的分布有依赖关系,并就此结果进行了讨论。

关键词： LH cell localization Pituitary portal vessel supply Rat horseradish peroxidase Immuno-labelling method.

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FUNCTIONAL　AND　STRUCTURAL　RELATION　BETWEEN　CENTRAL　CHEMORECEPTION　OF　RESPIRATION　AND　THE　NUCLEUS　RETROFACIALIS　IN　RATS

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Chinese Medical Sciences Journal 1994年第1期

作者：田国锋钱梓文张镜如

ＦＵＮＣＴＩＯＮＡＬＡＮＤＳＴＲＵＣＴＵＲＡＬＲＥＬＡＴＩＯＮＢＥＴＷＥＥＮＣＥＮＴＲＡＬＣＨＥＭＯＲＥＣＥＰＴＩＯＮＯＦＲＥＳＰＩＲＡＴＩＯＮＡＮＤＴＨＥ　ＮＵＣＬＥＵＳＲＥＴＲＯＦＡＣＩＡＬＩＳＩＮＲＡＴＳＴｉａ... 详细信息

ＦＵＮＣＴＩＯＮＡＬＡＮＤＳＴＲＵＣＴＵＲＡＬＲＥＬＡＴＩＯＮＢＥＴＷＥＥＮＣＥＮＴＲＡＬＣＨＥＭＯＲＥＣＥＰＴＩＯＮＯＦＲＥＳＰＩＲＡＴＩＯＮＡＮＤＴＨＥ　ＮＵＣＬＥＵＳＲＥＴＲＯＦＡＣＩＡＬＩＳＩＮＲＡＴＳＴｉａｎＧｏｕｆｅｎｇ；（田国锋）Ｑｉａ...

关键词： nucleus retrofacialis hydrogen ions horseradish peroxidase

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Microscopic Characteristics, Chromatographic Profiles and Inhibition of peroxidase Activity of the Leaves of <i>Manihot esculenta</i>and <i>Manihot glaziovii</i>, Consumed as Traditional Vegetables

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Journal of Biosciences and Medicines 2021年第9期9卷 59-73页

作者： Paulin Mutwale Kapepula Patricia Mbombo Mungitshi Dieudonné Tshitenge Tshitenge Thierry Franck Dieudonné Mumba Ngoyi Pascal Dibungi T. Kalenda Monique Tits Michel Frédérich Nadege Kabamba Ngombe Ange Mouithys-Mickalad Centre d’Etudes des Substances Naturelles d’Origine Végétale (CESNOV) Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of the Congo Centre for Oxygen Research and Development (CORD) University of Liège Liège Belgium Faculty of Medicine University of Kinshasa Kinshasa Democratic Republic of the Congo Laboratory of Pharmacognosy Center for Interdisciplinary Research on Medicines (CIRM) University of Liège Liège Belgium

Methanolic extracts from the leaves of Manihot esculenta (Two cultivars) and Manihot glaziovii, consumed as traditional vegetables in DR. Congo was chemically characterized by Thin layer Chromatography and High Performance Liquid Chromatography. In vitro biochemical activities of extracts against Radical Oxidative Species (ROS) production were assessed in cellular models, on enzymes, Myeloperoxidase (MPO) and horseradish peroxidase (HRP) involved in inflammation. The microscopic analysis of the powder of leaves showed that each species displays specific and discriminating botanical microscopic features. Varieties of M. esculenta had a chemical fingerprint different from M. glaziovii. The majority of compounds were polyphenols, represented mainly by rutin, kaempferol-3-O-rutinoside, amentoflavone, phenolic acids such as gallic acid. All extracts exhibited high cellular antioxidant activity in the range of 0.1 to 10 μg·mL-1 using lucigenin with neutrophils, but a moderate cellular antioxidant activity ranging between 10 and 100 μg·mL-1 with DCFDA on HL60 monocytes. Extracts from Manihot leaves showed a pronounced inhibitory effect on the production of extracellular ROS, on HRP and myeloperoxidase activity. Cellular antioxidant activities, the inhibitory effect on HRP of extracts from M. glaziovii, M. esculenta cultivar Mwambu were significantly higher, but their inhibitory effect on the activity of MPO was lower than those of M. esculenta cultivar TEM 419. The biological activities of Manihot esculenta and Manihot glaziovii were well correlated to their phytochemicals that could justify their traditional use as vegetables, potential functional foods or nutraceutical resources and medicines.

关键词： horseradish peroxidase Kahemba Konzo Manihot esculenta Manihot glaziovii Myeloperoxidase Traditional Vegetable

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Biosensor Sensitive to Hydrogen Peroxide via Methylene Blue Incorporated into Nafion Film as an Electron Transfer Mediator

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Advances in Manufacturing 1998年第2期 72-79页

作者： Wu Xinxin Ying Tailin Sun Kang (School of Chemistry and Chemical Engineering)

Biosensor sensitive to hydrogen peroxide was made via methylene blue in Nafion gel as an electron transfer mediator. Cyclic voltommetry and chronoamperometry were for the first time used to illustrate the suitability of electrical communication between immobilized horseradish peroxidase and a glassy carbon electrode via methylene blue incorporated into a (Nafion) perfluorosulfonic acid cation exchange polymer film. The methylene blue mediator was strongly reatined inside Nafion film through hydrophobic interactions. Effects of applied potential, ionic strength, temperature and pH on the biosensor were investigated. The biosensor possessed a variety of advantages including high sensitivity, long term stability and rapid response to hydrogen peroxide with a detection limit of 0.1 μmol/L. The high sensitivity of the biosensor was due to the high efficiency of electron transfer between immobilized horseradish peroxidase and the electrode via methylene blue.

关键词： biosensor horseradish peroxidase Nafion methylene blue hydrogen peroxide

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Phenolic Compounds Hybrid Detectors

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Journal of Biomaterials and Nanobiotechnology 2013年第3期4卷 17-27页

作者： Jadwiga Soloducho Joanna Cabaj Department of Medicinal Chemistry and Microbiology Faculty of ChemistryWroclaw University of TechnologyWroclawPoland

Phenolic compounds are among the major classes of pollutants produced by industrial and agricultural activities. The amperometric biosensors have been mainly applied to the determination of phenolic compounds because of the advantages such as good selectivity, low cost, and easy automation. Amperometry is a method to measure the electric current that flows as a result of reactions generated at the electrode. Amperometric phenol biosensors are most often based on tyrosinase, laccase or horseradish peroxidase immobilized on the electrode surface. The immobilization of enzymes into ordered thin materials has attracted considerable attention over the past few years. The present researches have demonstrated that biomolecules immobilized in different matrixes retain their functional characteristics to a large extent. These new materials are of great interest for applications as biosensors and biocatalysts. Lately, also conducting polymers have attracted much interest in the development of biological sensors. The electrically conducting polymers are known as possessing many interesting features, which allow them to act as excellent materials for immobilization of biomolecules.

关键词： Biosensors Phenolic Compound Laccase Tyrosinase horseradish peroxidase Thin Film Immobilization Conducting Polymers

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