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Point mutations of homologs as an adaptive solution to the gene loss

Journal of genetics and Genomics 2023年第7期50卷 511-518页

作者： Guosheng Ma Xiaojing Zhao Xinyi Shentu Liye Zhang School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Shanghai Clinical Research and Trial Center Shanghai 201210China State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China

gene loss is common and influences genome evolution trajectories.Multiple adaptive strategies to compensate for gene loss have been observed,including copy number gain of paralogous genes and mutations in genes of the same pathway.By using the Ubl-specific protease 2(ULP2)eviction model,we identify compensatory mutations in the homologous gene ULP1 by laboratory evolution and find that these mutations are capable of rescuing defects caused by the loss of ULP2.Furthermore,bioinformatics analysis of genomes of yeast gene knockout library and natural yeast isolate datasets suggests that point mutations of a homologous gene might be an additional mechanism to compensate for gene loss.

关键词： gene loss ULP2 homologous gene Compensatory mutation Genome evolution

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Functional Replacement of the FabD Protein of Escherichia coli Fatty Acid Synthesis by Eimeria tenella MCAT Homologue

Functional Replacement of the FabD Protein of Escherichia co...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次...

作者： Sun Ming-fei~(1,2),Zhu Guan~3,Qin Zong-hua~(1,2),Yuan Jian-feng~(1,2),Yu Jin-shu~(1,2),Lv Min-na~(1,2), Wu Cai-yan~(1,2),Xie Ming-quan~(1,2),Cai Jian-ping~(1,2) (1.Institute of Veterinary Medicine,Guangdong Academy of Agricultural Sciences,Guangzhou,510640 2.Guangdong Common Lab of Veterinary Public Health,Guangzhou,510640 3.Lab of Molecular Parasitology,School of Veterinary and Biomedicine Science,Texas A&M University,USA)

TypeⅡfatty acid biosynthetic system is an essential process for bacteria,apicomplexans and some plants survival.Undoubtedly,Escherichia coli provides the paradigm for the system.However,little was known about the fatty acid metabolism in Eimeria spp(apicomplexans).Here,we report a E.tenella homologous gene(EtMCAT) to E.coli fabD,the protein shows 37%homology to E.coli FabD amino acid sequence in the coding region for the mature protein.Additionally,the predicted structure of EtMCAT is similar to E.coli FabD crystal structure except for a few differences in secondary structure elements.Functional replacement of fabD gene with amber mutation of E.coli temperature-sensitive LA2-89 strain by E.tenella MCAT homologue using homologous recombination with suicide plasmid pDS132,the transgenic E.coli strain which fabD gene with an amber mutation was replaced by cDNA encoding mature E.tenella MCAT could restore growth at non-permissive temperature(39℃).The results demonstrated the functional complementation and direct function of EtMCAT.To our knowledge,this is the first functional evidence supporting the identification of E.tenella cDNA for MCAT.

关键词： Eimeria tenella Escherichia coli TypeⅡfatty acid biosynthetic homologous gene Functional replacement Malonyl-CoA:ACP transacylase

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