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A soluble pH-responsive host-guest-based nanosystem for homogeneous exosomes capture with high-efficiency

中国化学快报:英文版 2023年第8期34卷 296-301页

作者： Haiyan Wang Peng Liang Lei Zhang Liping Shi Yitong Ge Liyuan Zhang Xiaoqiang Qiao College of Pharmaceutical Sciences Key Laboratory of Public Health Safety of Hebei ProvinceKey Laboratory of Medicinal Chemistry and Molecular DiagnosisMinistry of EducationHebei UniversityBaoding 071002China College of Basic Medical Science Key Laboratory for Proteomics of Liaoning ProvinceDalian Medical UniversityDalian 116044China

Exosomes offer ideal biomarkers for liquid biopsies.However,high-efficient capture of exosomes has been proven to be extreme challenging.Here,we report a soluble pH-responsive host-guest-based nanosystem(pH-HGN)for homogeneous isolation of exosomes around physiological pH.The pH-HGN consists of two specifically functionalized modules.First,a pH-responsive module,poly-dimethylaminoethyl methacrylate,provides homogeneous capture circumstances and sharp pH-triggered self-assembly separation in aqueous solution to improve capture efficiency and reduce nonspecific adsorption.Second,a host-guest module,poly-acrylamide azobenzene andβ-cyclodextrin linked with exosomes-specific antibody,could act as the"cleavable bridge"to specific capture and subsequent rapid release of captured exosomes through host-guest interaction betweenβ-cyclodextrin and AAAB moieties.The pH-HGN offered high capture efficiencies for exosomes from two different cell lines,which were 90.2%±0.28%and 87.0%±4.6%for H1299 and MCF-7 cell-derived exosomes,respectively.The purity of isolated exosomes was(1.49±0.71)×10^(11)particles/μg,which was 4.1 times higher compared with the gold standard ultracentrifugation(UC)method.Furthermore,the isolated exosomes via the pH-HGN can preserve well integrity and biological activity.The developed pH-HGN was further successfully applied to differentiate lung cancer patients from healthy persons.These findings indicated that pH-HGN is a promising strategy in exosomes-based research and downstream applications.

关键词： Exosomes homogeneous capture Soluble nanosystem pH-responsive polymer Host-guest interaction

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