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Ultrastructure and histochemistry.of rat myocardial capillary endothelial cells in response to diabetes and hypertension

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Cell Research 2005年第7期15卷 532-538页

作者： Ludmila OKRUHLICOVA,Narcis TRIBULOVA1,Peter WEISMANN,Ruzena SOTNIKOVA Institute for Heart Research Slovak Academy of Sciences 840 05 Bratislava Slovak Republic Institute for Heart Research Slovak Academy of Sciences 840 05 Bratislava Slovak Republic Medical Faculty Comenius University Bratislava Slovak Republic Institute of Experimental Pharmacology Slovak Academy of Sciences Bratislava Slovak Republic

Insufficient growth and rarefaction of capillaries, followed by endothelial dysfunction may represent one of the mostcritical mechanisms involved in heart damage. In this study we examined histochemical and ultrastructural changes inmyocardial capillary endothelium in two models of heart failure streptozotocin-induced diabetes mellitus (STZ) and NO-deficient hypertension in male Wistar rats. Diabetes was induced by a single i.v. dose of STZ (45 mg/kg) and chronic 9-week stage was analysed. To induce NO-deficient hypertension, animals were treated with inhibitor of NO synthase L-nitroarginine methylester (L-NAME) (40 mg/kg) for 4 weeks. Left ventricular tissue was processed for enzyme cata-lytic histochemistry.of capillary alkaline phosphatase (AlPh), dipeptidyl peptidase IV (DPP IV), and endothelial NOsynthase/NADPH-diaphorase (NOS) and for ultrastructural analysis. In diabetic and hypertensive rats, lower/absentAlPh and DPP IV activities were found in focal micro-areas. NOS activity was significantly reduced and persisted onlylocally. Quantitative evaluation demonstrated reduction of reaction product intensity of AlPh, DPP and NOS by 49.50%,74.36%, 20.05% in diabetic and 62.93%, 82.71%, 37.65% in hypertensive rats. Subcellular alterations of endothelialcells were found in heart of both groups suggesting injury of capillary function as well as compensatory *** injury was more significant in diabetic animals, in contrast the adaptation was more evident in hypertensiveones. Concluding: both STZ-induced diabetes- and NO-deficient hypertension-related cardiomyopathy were accompa-nied by similar features of structural remodelling of cardiac capillary network manifested as angiogenesis and *** latter was however, predominant and may accelerate disappearance of capillary endothelium contributing to myo-cardial dysfunction.

关键词： rat heart diabetes NO-deficiency capillary ultrastructure histochemistry.

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Diagnostic histochemistry

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2008年

作者： Mark R. Wick

ISBN: (纸本)9780521874106

Pathology is an inherently subjective discipline, and therefore is often referred to as both an art and a science. Over the years, laboratory physicians have implemented special tissue stains and molecular techniques to limit subjectivity in the discipline. Beginning in the late 19th century, histochemical stains were developed to assess diagnostic biochemical reactions in tissue. histochemistry.has recently seen a resurgence in popularity because of the higher costs of other newer methods. Today, this technique is used by almost every pathology laboratory across the world. This book comprehensively covers all diseases for which that technique plays a central role in diagnosis. Every anatomic region is covered in detail with examples of appropriate staining techniques, and the book is heavily illustrated with over 850 color photomicrographs. This is the first monograph to be published on histochemistry.in 15 years, and it is the only one that is diagnostically-oriented.

关键词： histochemistry. Molecular diagnosis. Histocytochemistry Diagnosis. Histological Techniques Pathology.

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