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Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis

World Journal of Stem Cells 2019年第10期11卷 803-816页

作者： Jeong Ah Kim Soohyun Hong Won Jong Rhee Research Center for Bioconvergence Analysis Korea Basic Science InstituteCheongju 28119South Korea Department of Bio-Analytical Science University of Science and TechnologyDaejeon 34113South Korea Program in Biomicro System Technology Korea UniversitySeoul 02841South Korea Division of Bioengineering Incheon National UniversityIncheon 22012South Korea Department of Bioengineering and Nano-Bioengineering Incheon National UniversityIncheon 22012South Korea

Although the recent advances in stem cell engineering have gained a great deal of attention due to their high potential in clinical research,the applicability of stem cells for preclinical screening in the drug discovery process is still challenging due to difficulties in controlling the stem cell microenvironment and the limited availability of high-throughput systems.Recently,researchers have been actively developing and evaluating three-dimensional(3D)cell culture-based platforms using microfluidic technologies,such as organ-on-a-chip and organoid-on-a-chip platforms,and they have achieved promising breakthroughs in stem cell engineering.In this review,we start with a comprehensive discussion on the importance of microfluidic 3D cell culture techniques in stem cell research and their technical strategies in the field of drug discovery.In a subsequent section,we discuss microfluidic 3D cell culture techniques for high-throughput analysis for use in stem cell research.In addition,some potential and practical applications of organ-on-a-chip or organoid-on-a-chip platforms using stem cells as drug screening and disease models are highlighted.

关键词： Stem cell Microfluidic technology Three-dimensional cell culture highthroughput screening

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LABEL-FREE DETECTION OF PROTEIN MICROARRAY WITH HIGH THROUGHPUT SURFACE PLASMON RESONANCE IMAGING(SPRI)

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Journal of Innovative Optical Health Sciences 2008年第1期1卷 107-114页

作者： SHUKUAN XU GUOLIANG HUANG CHENG DENG JIANG ZHU CHAO HAN XIAOYONG YANG Medical Systems Biology Research Center Tsinghua University School of Medicine Beijing China National Engineering Research Center for Beijing Biochip Technology BeijingChina

A surface plasmon resonance imaging(SPRI)system was developed for the discrimination of proteins on a gold surface.As a label-free and high-throughput technique,SPRI enables simultaneously monitoring of the biomolecular interactions at low concentrations.We used SPRI as a label-free and parallel method to detect different proteins based on protein microarray.Bovine Serum Albumin(BSA),Casein and Immunoglobulin G(IgG)were immobilized onto the Au surface of a gold-coated glass chip as spots forming a 6×6 matrix.These proteins can be discriminated directly by changing the incident angle of light.Excellent reproducibility for label-free detection of protein molecules was achieved.This SPRI platform represents a simple and robust method for performing high-sensitivity detection of protein microarray.

关键词： Surface plasmon resonance imaging protein microarray label-free highthroughput

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Familial Alzheimer's disease modelling using induced pluripotent stem cell technology

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World Journal of Stem Cells 2014年第2期6卷 239-247页

作者： Lisa Mohamet Natalie J Miazga Christopher M Ward Stem Cell Biology Group Core Technology FacilityFaculty of Human and Medical SciencesThe University of Manchester

Alzheimer’s disease(AD)is a progressive neurodegenerative disease in which patients exhibit gradual loss of memory that impairs their ability to learn or carry out daily tasks.Diagnosis of AD is difficult,particularly in early stages of the disease,and largely consists of cognitive assessments,with only one in four patients being correctly diagnosed.Development of novel therapeutics for the treatment of AD has proved to be a lengthy,costly and relatively unproductive process with attrition rates of>90%.As a result,there are no cures for AD and few treatment options available for patients.Therefore,there is a pressing need for drug discovery platforms that can accurately and reproducibly mimic the AD phenotype and be amenable to high content screening applications.Here,we discuss the use of induced pluripotent stem cells(iPSCs),which can be derived from adult cells,as a method of recapitulation of AD phenotype in vitro.We assess their potential use in high content screening assays and the barriers that exist to realising their full potential in predictive efficacy,toxicology and disease modelling.At present,a number of limitations need to be addressed before the use of iPSC technology can be fully realised in AD therapeutic applications.However,whilst the use of AD-derived iPSCs in drug discovery remains a fledgling field,it is one with immense potential that is likely to reach fruition within the next few years.

关键词： Human induced pluripotent stem cells Alzheimer’s disease Neurodegenerative diseases highthroughput screening assays Cholinergic neurons Drug discovery Stratified medicine

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