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Cell-PLoc 2.0: an improved package of web-servers for predicting subcellular localization of proteins in various organisms

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Natural Science 2010年第10期2卷 1090-1103页

作者： Kuo-Chen Chou Hong-Bin Shen 不详

Cell-PLoc 2.0 is a package of web-servers evolved from Cell-PLoc (Chou, K.C. & Shen, H.B., Nature Protocols, 2008, 2:153-162) by a top-down approach to improve the power for predicting subcellular localization of proteins in various organisms. It contains six predictors: Euk-mPLoc 2.0, Hum-mPLoc 2.0, Plant-mPLoc, Gpos-mPLoc, Gneg-mPLoc, and Virus-mPLoc, specialized for eukaryotic, human, plant, Gram- positive bacterial, Gram-negative bacterial, and virus proteins, respectively. Compared with Cell-PLoc, the predictors in the Cell-PLoc 2.0 have the following advantageous features: (1) they all have the capacity to deal with the multiplex proteins that can simultaneiously exist, or move between, two or more subcellular location sites;(2) no accession number is needed for the input of a query protein even if using the “high- level” GO (gene ontology) prediction engine;(3) the functional domain information and sequential evolution information are fused into the “ab initio” sequence-based prediction engine to enhance its accuracy. In this protocol, a step- to-step guide is provided for how to use the web server predictors in the Cell-PLoc 2.0 package, which is freely accessible to the public at http://***/bioinf/Cell-PLoc-2/.

关键词： Euk-mPLoc 2.0 Hum-mPLoc 2.0 Plant-mPLoc Gpos-mPLoc Gneg-mPLoc Virus-mPLoc higher-level GO approach Ab-initio approach Functional domain Sequential evolution

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Teaching Reading Skills through the Processes Involved in Reading

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海外英语 2018年第21期 261-263页

作者：屈明欣新航道英语培训机构

In English as Second language reading research, how can the processes involved in reading make contribution to teachingof reading skills has long been discussed. In this essay, processes of reading as well as the applications in the classroom are illus-trated. The first section of this essay will look at some basic or background information about reading process, such as basic con-cepts and importance of lower-level and higher-level processes. The second section will discuss critically lower-level processsuch as word recognition, grammatical knowledge and lexical access. Whether these components of lower-level process have an ef-fect on EFL or ESL advanced learners and how to take these factors into consideration while teaching reading. The third sectionwill talk about several components of higher-level process such as schemata and strategy

关键词： reading processes lower-level process higher-level processes

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