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The Crop Journal 2023年第1期11卷 33-45页

作者： Aamir Hamid Khan Yizan Ma Yuanlong Wu Adnan Akbar Muhammad Shaban Abid Ullah Jinwu Deng Abdul Saboor Khan Huabin Chi Longfu Zhu Xianlong Zhang Ling Min National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina Department of Botany University of MalakandDir Lower 18800Khyber PakhtunkwaPakistan

Cotton(Gossypium spp.) yield is reduced by stress. In this study, high temperature(HT) suppressed the expression of the jasmonic acid(JA) biosynthesis gene allene oxide cyclase 2(GhAOC2), reducing JA content and causing male sterility in the cotton HT-sensitive line H05. Anther sterility was reversed by exogenous application of methyl jasmonate(MeJA) to early buds. To elucidate the role of GhAOC2 in JA biosynthesis and identify its putative contribution to the anther response to HT, we created gene knockout cotton plants using the CRISPR/Cas9 system. Ghaoc2 mutant lines showed male-sterile flowers with reduced JA content in the anthers at the tetrad stage(TS), tapetum degradation stage(TDS), and anther dehiscence stage(ADS). Exogenous application of MeJA to early mutant buds(containing TS or TDS anthers) rescued the sterile pollen and indehiscent anther phenotypes, while ROS signals were reduced in ADS anthers. We propose that HT downregulates the expression of GhAOC2 in anthers, reducing JA biosynthesis and causing excessive ROS accumulation in anthers, leading to male sterility. These findings suggest exogenous JA application as a strategy for increasing male fertility in cotton under HT.

关键词： Cotton(Gossypium hirsutum) Jasmonic acid Allene oxide cyclase 2 ROS CRISPR/Cas9 high-temperature stress

Osmotic Regulation,Antioxidant Enzyme Activities and Photosynthetic Characteristics of Tree Peony(Paeonia suffruticosa Andr.)in Response to high-temperature stress

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Phyton-International Journal of Experimental Botany 2023年第11期92卷 3133-3147页

作者： Zhipeng Sheng Jiasong Meng Jun Tao Daqiu Zhao College of Horticulture and Landscape Architecture Yangzhou UniversityYangzhou225009China

Tree peony(Paeonia suffruticosa Andr.)is a traditional Chinese flower,which prefers cool ***,high temperature in summer in the middle and lower reaches of the Yangtze River restricts its growth and *** this study,osmotic regulation,antioxidant enzyme activities,and photosynthetic characteristics of tree peony in response to high-temperature stress were *** results showed that high-temperature stress had destroyed the cell membrane,manifested as the increased relative electrical conductivity and malondialdehyde ***,high-temperature stress led to excessive accumulation of reactive oxygen species,thereby,activating antioxidant enzyme ***,photosynthetic parameters and chlorophyll fluorescence parameters directly reflected the damage to the photosystem II reflection center under high-temperature *** addition,high-temperature stress led to stomatal closure and chloroplast *** study revealed the physiological responses of tree peony to high-temperature stress,laying a foundation for the promotion of tree peony in high-temperature areas and the improvement of high-temperature resistance.

关键词： Tree peony high-temperature stress antioxidant enzyme photosynthetic characteristics

Physiological Response of Spring Soybean Seedlings Under high-temperature stress

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Journal of Northeast Agricultural University(English Edition) 2021年第3期28卷 1-8页

作者： Dong Shou-kun Hou Xiao-min Wang Xi-yue Wu Zi-hao Ma Ze-zhong Li Shuang Liu Li-jun College of Agriculture Northeast Agricultural UniversityHarbin 150030China

To understand the response characteristics of soybean seedling resistance systems to high-temperature stress,the spring soybean varieties HN44 and HN65 were selected in this study as the experimental materials,as they had substantial differences in stress *** physiological status was studied under high-temperature *** culture was used in the experiment,and soybeans at the seedling stage were treated with high-temperature *** results showed that the activity of antioxidases and osmotic regulatory substance contents in soybean increased under high-temperature treatment for a certain period of time,the activity of antioxidases and osmotic regulatory substance contents decreased after continuous treatment,and high temperatures continuously increased malondialdehyde contents in the two *** high temperatures,the antioxidant activity and osmotic regulator contents of HN44 were higher than those of HN65,and the malondialdehyde contents of HN44 were higher than those of *** above results showed that HN44 soybeans exhibited significantly higher resistance to high-temperature stress than HN65 variety.

关键词： soybean high-temperature stress antioxidase osmotic regulatory substance

Rice Heat Tolerance Breeding: A Comprehensive Review and Forward Gaze

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Rice science 2024年第4期31卷 375-400,I0022页

作者： Ravindran Lalithambika VISAKH Sreekumar ANAND Sukumaran Nair ARYA Behera SASMITA Uday Chand JHA Rameswar Prasad SAH Radha BEENA Department of Genetics and Plant Breeding College of AgricultureVellayaniKerala Agricultural UniversityThiruvananthapuramKerala 695522India Crop Improvement Division Indian Council of Agricultural Research-National Rice Research InstituteCuttackOdisha 7530006India Department of Plant Breeding and Genetics Indian Institute of Pulse ResearchKanpur 208024India Department of Plant Physiology College of AgricultureVellayaniKerala Agricultural UniversityThiruvananthapuramKerala 695522India

The yield potential of rice is seriously affected by heat stress due to climate change. Since rice is a staple food globally, it is imperative to develop heat-resistant rice varieties. Thus, a thorough understanding of the complex molecular mechanisms underlying heat tolerance and the impact of high temperatures on various critical stages of the crop is needed. Adoption of both conventional and innovative breeding strategies offers a long-term advantage over other methods, such as agronomic practices, to counter heat stress. In this review, we summarize the effects of heat stress, regulatory pathways for heat tolerance, phenotyping strategies, and various breeding methods available for developing heat-tolerant rice. We offer perspectives and knowledge to guide future research endeavors aimed at enhancing the ability of rice to withstand heat stress and ultimately benefit humanity.

关键词： genetic mechanism high-temperature stress molecular breeding genomics selection

Superoxide dismutase genes in Pyropia haitanensis:molecular cloning,characterization and mRNA expression

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Acta Oceanologica Sinica 2016年第6期35卷 101-111页

作者： JI Dehua XU Yan XIAO Haidong CHEN Changsheng XU Kai XIE Chaotian Fisheries College Jimei University

Superoxide dismutase（SOD,EC 1.***.1.1） is the first and most important line of cellular defense against oxidative *** this study,based on unigene sequences of ***,three full-length Ph SOD genes were obtained by RACE technology,and named Ph MSD,Ph CSD1 and Ph *** full-length c DNAs of these genes comprised973,1 029 and 954 nucleotides,*** c DNAs encoded proteins of 224,134 and 216 amino acids,with isoelectric points of 5.75,4.65 and 10.74,*** on their conserved motifs and phylogenetic tree analysis,the three Ph SODs were divided into two SOD types：Ph MSD is a Mn-SOD,and Ph CSD1 and Ph CSD2 are Cu Zn-SODs.q PCR was used to measure the expression of the three Ph SOD genes in different life phases of ***,and under different periods of high-temperature stress and different levels of *** the different life phases of ***,the expression levels of the three Ph SODs were all significantly higher in the thallus than in the *** high-temperature and desiccation stress,the expression levels of Ph CSD1 and Ph CSD2 were highly induced by the O2-content,but the expression level of Ph MSD was highly depressed by high temperature stress and showed no significant change during desiccation.

关键词： superoxide dismutase gene cloning desiccation stress high-temperature stress qPCR