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iTRAQ-based proteomics reveals the mechanism of action of Yinlai decoction in treating pneumonia in mice consuming a high-calorie diet

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Journal of Traditional Chinese Medical Sciences 2024年第1期11卷 21-32页

作者： Qianqian Li Tiegang Liu Chen Bai Xueyan Ma Hui Liu Zi’an Zheng Yuxiang Wan He Yu Yuling Ma Xiaohong Gu School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 102488China Institute of Chinese Medicine Epidemic Disease Beijing University of Chinese MedicineBeijing 102488China School of Traditional Chinese Medicine Guizhou University of Traditional Chinese MedicineGuiyang 550025China Department of Acupuncture and Mini-invasive Oncology Beijing University of Chinese Medicine Third Affiliated HospitalBeijing 100029China OCMRC Center University of OxfordOxford OX13PTUnited Kingdom

Objective:To uncover the underlying mechanisms of action of the Yinlai decoction on high-calorie dietinduced pneumonia through proteomics ***:Based on the Gene Expression Omnibus(GEO)database,lung tissue samples from normal and high-fat diet(HFD)fed mice in the GSE16377 dataset were selected as test cohorts to identify differentially expressed genes and conduct bioinformatics *** the animal experiments,mice were randomly divided into the control(N),high-calorie diet pneumonia(M),and Yinlai decoction treatment(Y)*** in the M group received high-calorie feed and a 0.5 mg/mL lipopolysaccharide solution spray for 30 min for 3 *** mice in the Y group were intragastrically administered 2 mL/10 g Yinlai decoction twice daily for 3 *** evaluation of the lung tissue was *** expressed proteins(DEPs)in the lung tissue were identified using quantitative proteomics and bioinformatics *** drug-target relationships between Yinlai decoction and core DEPs in the lung tissue were verified using AutoDock Vina and Molecular Graphics Laboratory(MGL)*** were verified by western ***:GEO data mining showed that an HFD altered oxidative phosphorylation in mouse lung *** Yinlai decoction alleviated pathological damage to lung tissue and pneumonia in mice that were fed a high-calorie diet.A total of 47 DEPs were identified between the Y and M *** analysis revealed their association with energy metabolism pathways such as the tricarboxylic acid cycle(TCA)and oxidative *** protein-protein interaction network revealed that Atp5a1,Pdha1,and Sdha were the target proteins mediating the therapeutic effects of Yinlai *** docking results suggested that the mechanism of the therapeutic effect of Yinlai decoction involves the binding of brassinolide,praeruptorin B,chrysoeriol,and other components in Yinlai decoction to ***:The Yinlai decoction allevia

关键词： Yinlai decoction high-calorie diet Pneumonia iTRAQ proteomics Energy metabolism

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Effect of Yinlai Decoction on the metabolic pathways in the lung of high-calorie diet-induced pneumonia rats

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Journal of Traditional Chinese Medical Sciences 2021年第1期8卷 4-16页

作者： Fuyang Xian Tiegang Liu Chen Bai Guannan Yang Xueyan Ma Bochuan Wang Ling Huang Shaoyang Liu Jianhua Zhen Jianzhen He He Yu Yuling Ma Taiyi Wang Xiaohong Gu School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 102488China OCMRC Center University of OxfordOxford OX13PTUK

Objective:To search for specific metabolites in the lungs of pneumonia rats fed with a high-calorie diet,as well as explore the changes in the lung metabolites of young rats treated with Yinlai Decoction(YD)and its effects on inflammation-related metabolic ***:Lipopolysaccharides(LPS)and a special high-calorie diet were used to induce Sprague Dawley(SD)rats to simulate the intestinal state of infant *** chromatography-mass spectrometry technology(LC-MS/MS)was used to detect metabolites in each *** orthogonal partial least squares discriminant analysis(OPLS-DA)model values were used for the detection results to find the differential *** metabolic pathways that are involved with the differential metabolites were clarified through enrichment analysis and topological ***,the T cell receptor signaling pathway(TCR)signal conversion was analyzed by the network pharmacology ***:In the high-calorie diet combined with pneumonia group(M3),a total of 55 metabolites were determined to be different from the normal group(N).A total of 36 metabolites were determined to be different from those in the lung metabolites of the YD treatment group(T1).YD had a regulatory effect on glutathione metabolism,arginine and proline metabolism,ascorbic acid and aldehyde metabolism and phenylalanine *** the small molecule metabolites could act on the FYN and lymphocytespecific protein tyrosine kinase(LCK)target proteins in the TCR signaling pathway,thereby affecting the immune function of the ***:A high-calorie diet can cause abnormal sphingolipid metabolism in the lungs of young rats,thereby creating chronic lung inflammation in young *** has a beneficial effect when used to treat young rats with LPS-induced pneumonia fed on high-calorie *** mechanisms of action may affect the body’s immune pathways by regulating the oxidative stress pathway affected by glutathione metabolism.

关键词： high-calorie diet Pneumonia Yinlai decoction Metabolomics Network pharmacology

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