检索结果-南通市图书馆

high-affinity T cell receptors redirect cytokine-activated T cells (CAT) to kill cancer cells

Frontiers of Medicine 2019年第1期13卷 69-82页

作者： Synat Kang Yanyan Li Yifeng Bao Yi Li State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China University of Chinese Academy of Sciences Beijing 100049China

Cytokine-activated T cells (CATs) can be easily expanded and are widely applied to cancer immunotherapy. However, the good efficacy of CATs is rarely reported in clinical applications because CATs have no or very low antigen specificity. The low-efficacy problem can be resolved using T cell antigen receptorengineered CAT (TCR-CAT). Herein, we demonstrate that NY-ESO-1157-165 HLA-A*02:01-specific high-affinity TCR (HAT)-transduced CATs can specifically kill cancer cells with good efficacy. With low micromolar range dissociation equilibrium constants, HAT-transduced CATs showed good specificity with no off-target killing. Furthermore, the high-affinity TCR-CATs delivered significantly better activation and cytotoxicity than the equivalent TCR-engineered T cells (TCR-Ts) in terms of interferon-γ and granzyme B production and in vitro cancer cell killing ability. TCR-CAT may be a very good alternative to the expensive TCR-T, which is considered an effective personalized cyto-immunotherapy.

关键词： cytokine-activated T cells high-affinity T cell receptor cancer immunotherapy TCR-CAT

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular recognition of cyclophanes in water

引用

Chinese Chemical Letters 2024年第5期35卷 32-44页

作者： Fang-Yuan Chen Wen-Chao Geng Kang Cai Dong-Sheng Guo College of Chemistry State Key Laboratory of Elemento-Organic ChemistryKey Laboratory of Functional Polymer Materials(Ministry of Education)Frontiers Science Center for New Organic MatterCollaborative Innovation Center of Chemical Science and Engineering(Tianjin)Nankai UniversityTianjin 300071China College of Chemistry Nankai UniversityTianjin 300071China

Molecular recognition in water,the biological solvent,always receives significant research focus in supramolecular *** mechanisms of molecular recognition in water is key to comprehending biological processes at the molecular *** the past five decades,supramolecular chemists have developed a vast array of synthetic receptors with highly diverse structures and recognition *** them,cyclophanes represent an important family of macrocyclic receptors that have been extensively *** aromatic moieties in cyclophanes not only facilitate chemical modifications to impart water solubility but also enable forming hydrophobic cavities for guest inclusion in aqueous *** by Koga et al.,who reported the first inclusion complex of cyclophanes in water and solid state,numerous water-soluble cyclophanes,including derivatives of blue box,calixarenes,resorcinarenes,pillararenes,octopusarenes,biphenarenes,coronarenes,and naphthotubes,etc.,have been synthesized and subjected to investigation of the recognition capabilities in aqueous *** review provides an overview of cyclophane receptors designed to bind organic guests in *** categorize them into two classes based on the modifications made to their hydrophobic cavities:those with“exo-functionalized hydrophobic cavities”and those with“endo-functionalized hydrophobic cavities”.We introduce their distinctive features and discuss strategies to enhance recognition affinity and *** review aims to inspire the development of novel synthetic receptors with intriguing properties and foster practical applications of cyclophanes.

关键词： Biomimetic receptor Cyclophane Molecular recognition Hydrophobic effect high-affinity Macrocycles

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

微囊藻毒素诱导小Mafk蛋白在淡水育珠蚌褶纹冠蚌中对GST的转录调控

微囊藻毒素诱导小Mafk蛋白在淡水育珠蚌褶纹冠蚌中对GST的转录调控

引用

2017年中国水产学会学术年会

作者：王晓波胡宝庆文春根南昌大学生命科学学院

小Maf蛋白MafF、MafG和MafK通过与转录因子的CNC家族结合在形态发生和体内平衡方面发挥关键作用。小Maf蛋白已经在脊椎动物中被报道具有很多功能。本研究克隆出了一种褶纹冠蚌的小Maf蛋白（CpMafK）,用过RACE技术获得了CpMafK的全长。Cp... 详细信息

小Maf蛋白MafF、MafG和MafK通过与转录因子的CNC家族结合在形态发生和体内平衡方面发挥关键作用。小Maf蛋白已经在脊椎动物中被报道具有很多功能。本研究克隆出了一种褶纹冠蚌的小Maf蛋白（CpMafK）,用过RACE技术获得了CpMafK的全长。CpMafK全长2170bp,其中开放阅读框（ORF）的碱基数为570bp,编码190个氨基酸。CpMafK具有四个保守结构域,与其他物种的小Mafs具有较低的序列相似性（54-63%）。实时定量PCR结果显示,CpMafKmRNA在组织中均有表达,最高表达水平在肝胰腺。在微囊藻毒素（MC）刺激后,CpMafKmRNA的表达水平在血细胞和肝胰腺中上调。将CpMafK的cDNA克隆到质粒pET-32中,重组蛋白在大肠杆菌BL21（DE3）中表达。CpMafK可以与CpGST1和CpGST2的启动子以高亲和力体外结合。

关键词： Cristaria plicata small Mafs cloning high-affinity

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：