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Clinical significance of extended high-risk human papillomavirus genotyping and viral load in cervical cancer and precancerous lesions

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Gynecology and Obstetrics Clinical Medicine 2023年第1期3卷 22-29页

作者： Pingping Su Jincheng Ma Lirui Yu Shuting Tang Pengming Sun Laboratory of Gynecologic Oncology Department of GynecologyFujian Maternity and Child Health HospitalCollege of Clinical Medicine for Obstetrics&Gynecology and PediatricsFujian Medical UniversityFuzhou350001FujianChina Fujian Key Laboratory of Women and Children's Critical Diseases Research Fujian Maternity and Child Health HospitalAffiliated Hospital of Fujian Medical UniversityFuzhouFujian350001China Fujian Clinical Research Center for Gynecological Oncology Fujian Maternity and Child Health Hospital(Fujian Obstetrics and Gynecology Hospital)Fuzhou350001FujianChina

Persistent infections with specific high-risk human papillomavirus(HR-HPV)strains are the leading cause of cervical cancer and precancerous lesions.HPV-16 and HPV-18 are associated with more than 70%of cervical cancer.However,with recent widespread vaccination efforts against cervical cancer,the infection rates of HPV-16 and HPV-18 have decreased across all age groups,while the infection rates of other HR-HPV strains have increased.The non-16/18 HR-HPV strains play an important role in cervical lesions.These strains can be identified with extended genotyping,and the 2019 American Society for Colposcopy and Cervical Pathology(ASCCP)guidelines recommended an HPV-based testing to assess the risk of cervical disease in patients.We reviewed and analyzed the clinical benefits of applying extended HR-HPV genotyping,which was published by the International Agency for Research on Cancer(HPV-16,18,31,33,35,39,45,51,52,56,58,59,66,and 68),to cervical cancer screening.This review concluded that cervical cancer screening needs to include extended HR-HPV genotyping.The examination of extended HR-HPV genotyping in cervical intraepithelial lesions and cervical cancers can help guide clinical practices.

关键词： high-risk human papillomavirus Cervical cancer Genotype Viral load Cancer screening

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Evaluation of dry specimen transport and processing time using an isothermal amplification high-risk human papillomavirus assay

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Gynecology and Obstetrics Clinical Medicine 2022年第1期2卷 34-37页

作者： Longhua Hu Suhui Wu Haixia Shang Jerome Belinson Jinhong Li Xiaoqin Wu Yaling Huang Jingfen Sun Department of Obstetrics and Gynecology Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Obstetrics and Gynecology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Preventive Oncology International Inc. ClevelandOHUSA Women's Health Institute Cleveland ClinicClevelandOHUSA

Background:Dry specimen transport has shown equivalence to traditional liquid transport using a novel high-risk human papillomavirus assay.Considering that dry transport might cross obstacles during cervical cancer screening in low and middle resource settings,this study was designed evaluate different processing time of dry specimen transport using the same isothermal amplification hrHPV assay.Methods:There were 564 women between the ages of 30–55 recruited from colposcopy clinic.For each patient,two endocervical samples were collected and placed into empty collection tubes by physician.Samples were stored at room temperature until analyzed for hrHPV using the AmpFire assay at two time points:2 days and 2 weeks.511 of the 564 participants with positive hrHPV were provided colposcopy exam and quadrant biopsy.Results:A total of 1128 endocervical samples from 564 patients were detected by the Ampfire assay.Good agreement was found between two time periods(KappaStandard error=0.67±0.04).Sensitivity(2days/2weeks)for CIN2t was 95.28%(95%CI:92.14%–98.42%)vs 90.57%(CI(86.65%–94.49%)and specificity(2days/2weeks)was 22.47%(CI 19.33%–25.61%)vs 28.15%(CI 24.23%–32.07%)respectively.The difference for Ampfire HPV detection in sensitivity for CIN2t for the two time periods was not significant(P=0.227),while the difference in specificity for CIN2t was significant(P=0.001).The difference in Ct values 29.23(CI 28.15–30.31)and 29.27(CI 28.19–30.35)between two time points was not significant(P?0.164).Conclusion:Processing dry brush specimens can be delayed up to 2 weeks.Using the AmpFire assay platform which supports cervical cancer prevention programs in low-to-middle-income countries(LMICs).

关键词： Cervical cancer Screening high-risk human papillomavirus Specimen transport Processing time Sample preparation

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Efficacy of 2LPAPI^®, a Micro-Immunotherapy Drug, in Patients with high-risk papillomavirus Genital Infection

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Advances in Infectious Diseases 2016年第1期6卷 7-14页

作者： Gilles Thomas Hélène Cluzel Jacqueline Lafon Jacques Bruhwyler Béatrice Lejeune Private Gynecology Practice Perpignan France Labo’Life France sarl Pescalis Les Magny France ECSOR sa/nv Blanmont Belgium Labo’Life Belgium sprl Les Isnes Belgium

human papillomaviruses (HPVs) are well known for being linked to the development of cervical cancers, most of them being caused by the high-risk (HR) oncogenic genotypes, mainly 16 and 18. The efficacy of 2LPAPI® (Labo’Life), a micro-immunotherapy homeopathic drug, has been evaluated in HR-HPV infected women (n = 18), in a private gynecology practice, by comparing them to an untreated control group (n = 18). Patients were 20 to 45 years old and had cytology with Atypical Squamous Cells of Undetermined Significance (ASCUS) or Low grade Superficial Intra Lesions/ Cervical Intraepithelial Neoplasia Grade I (LSIL/CINI). Patients freely chose to be treated with the drug or not. Those deciding not to take the drug remained untreated and were followed as a control group. The drug was taken at the regimen of one capsule per day during 6 months. HR-HPV and cytology were evaluated at 6 and 12 months. After 12 months, HR-HPV was cleared in 78% of the patients taking the drug versus 44% in those not taking it (p = 0.086). In patients over 25 years, HR-HPV clearance in the treated group was significantly higher (81.3%) than in the control group (20%) (p = 0.004). The difference in the regression of the lesion grades almost reached statistical significance (p = 0.053). This follow-up confirms that the micro-immunotherapy drug 2LPAPI® is a safe and effective therapeutic approach to treat HR-HPV cervical lesions in women over 25 years.

关键词： high-risk human papillomavirus Micro-Immunotherapy Genital Infection Homeopathy 2LPAPI®

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Correlation of HPV infection with TLRs pathway activation and downstream gene expression in cervical precancerous lesions

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Journal of Hainan Medical University 2018年第12期24卷 63-66页

作者： Bilikezi·aikemu Wei Gong Bahargul·ismawyil Ming-Ming Yue Department of Gynecology Affiliated Traditional Chinese Medicine HospitalXinjiang Medical UniversityUrumqi 830000XinjiangChina Department of Gynecology Urumqi Traditional Chinese Medicine Hospital in XinjiangUrumqi 830000XinjiangChina School of Traditional Chinese Medicine Xinjiang Medical UniversityUrumqi 830000XinjiangChina

Objective: To study the correlation of HPV infection with TLRs pathway activation and downstream gene expression in cervical precancerous lesions. Methods: Cervical precancerous lesion tissues from surgical resection or cone biopsy in Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University between June 2014 and May 2017 were collected and divided into HR-HPV positive group and HR-HPV negative group according to the condition of high-risk HPV infection;normal cervical tissues surgically removed due to fibroid in Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University during the same period were collected as the control group. The contents of TLRs pathway molecules as well as the expression of apoptosis-related genes and invasion-related genes in the cervical precancerous lesion tissues and normal cervical tissues were determined. Results:TLR2, TLR3, TLR4, TLR9 and NF-κB contents as well as CyclinD1, PCNA, iNOS, Gal-9, N-cadherin, HIF-1α and VEGF mRNA expression in HR-HPV positive group and HR-HPV negative group were significantly higher than those in control group whereas p53, Beclin1, LC3-II and SFRP2 mRNA expression were significantly lower than those in control group, and TLR2, TLR3, TLR4, TLR9 and NF-κB contents as well as CyclinD1, PCNA, iNOS, Gal-9, N-cadherin, HIF-1α and VEGF mRNA expression in HR-HPV positive group were significantly higher than those in HR-HPV negative group whereas p53, Beclin1, LC3-II and SFRP2 mRNA expression were significantly lower than those in HR-HPV negative group. Conclusion: HPV infection in cervical precancerous lesions can regulate downstream apoptosis and invasion gene expression by activating multiple TLRs pathways.

关键词： Cervical cancer Precancerous lesion high-risk human papillomavirus Toll-like receptor

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Diagnostic Accuracy of high-risk HPV Genotyping in Women with high-Grade Cervical Lesions:Evidence for renewed the Cervical Cancer Screening Strategy in China

Diagnostic Accuracy of High-risk HPV Genotyping in Women wit...

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浙江省免疫学会第十次学术大会

作者： Huihui Xu Aifen Lin Weiwu Shi Yang Zhang Xiujuan Shao Weihua Yan Medical Research Center Taizhou Hospital of Zhejiang ProvinceWenzhou Medical University human Tissue Bank Taizhou Hospital of Zhejiang ProvinceWenzhou Medical University Department of gynecology Taizhou Hospital of Zhejiang ProvinceWenzhou Medical University

Currently,clinical data for primary HPV screening alone are lacking in China.Here,we evaluate cervical cancer screening with primary HPV genotyping,as well as possible future screening strategy.Overall,high-risk HPV(hrHPV) prevalence was 18.2%among hospital-based population in Taizhou area.For cervical intraepithelial neoplasia 2 or worse(CIN2+),the sensitivity of primary hrHPV genotyping strategy and current cervical cancer screening strategy were 93.5%,and47.8%,respectively;whereas the specificity was 17.5%,and 69.1%,respectively.Current cervical screening strategy had slightly higher positive predictive values(28.7%) for CIN2+ than hrHPV genotyping strategy(22.8%),whereas primary hrHPV genotyping strategy demonstrated higher negative predictive values(91.2%)than current cervical screening strategy(83.5%).Compared to HPV35/39/45/51/56/59/66/68 genotypes,the odds ratios(OR) for CIN2+ in HPV16/18/31/33/52/58 infection women were 3.2(95%confidence interval[CI]2.3-4.1).Primary hrHPV genotyping strategy provides a better predictive value than HPV 16/18 genotyping alone in guiding the clinical management of the current cervical cancer screening.HPV testing without adjunctive cytology may be sufficiently sensitive for primary cervical cancer screening.

关键词： high-risk human papillomavirus genotyping diagnosis high-grade cervical lesion cervical cancer screening

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