检索结果-南通市图书馆

De Novo Transcriptome Analysis in hevea brasiliensis to Unveil Genes Involved in Low Temperature Stress Response

在线全文

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2023年第2期92卷 559-575页

作者： Bingsong Yang Xiaomeng Shi Tingting Guo Meixia Liang College of Agriculture Ludong UniversityYantai264025China The Engineering Research Institute of Agriculture and Forestry Ludong UniversityYantai264025China

Low temperature is one of the adversities threatening the growth and development and reduces the yield of rubber ***,molecular mechanisms toward rubber trees in response to low temperature are largely *** this study,7,159 and 7,600 differentially expressed genes(DEGs)were identified in‘Reyan 73397’rubber *** GO analysis,the catalytic activity was the representative of the GO term in the only DEGs at the two studied temperatures(room temperature and 4°C,respectively),while KEGG analysis showed that carbon metabolism was the most important grouping under the comparison of these two *** addition,expression of 9 members of transcription factor MYB family genes were further verified by qRT-PCR,and MYB family genes may play important roles in the regulation of rubber trees under low temperature *** study provided a theoretical foundation for(1)revealing the molecular mechanisms of rubber trees in response to low temperature and(2)breeding of tolerant varieties of rubber trees.

关键词： hevea brasiliensis low temperature stress transcriptome differential expressed genes

Intra-and inter-clonal tree growth variations of hevea brasiliensis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Forestry Research 2012年第3期23卷 429-434页

作者： H.R.Naji M.H.Sahri Department of Forest Production Faculty of ForestryUniversiti Putra MalaysiaSelangor43400Malaysia Department of Forestry Faculty of AgricultureIlam UniversityIlam69315-516Iran

We evaluated the effects of planting densities （500, 1,000, 1,500 and 2,000 ***-1） on tree growth performance （diameter at base, diameter at breast height, and clear bole height） of two clones （RRIM 2020 and PRIM 2025） of nine years old plantations of rubber tree （hevea brasiliensis Muell. Arg） in Malaysia. For the four planting densities of the two clones, basal area and diameter at breast height declined with increasing planting density. Clear bole heights were greatest at 1,500 *** lowest at 500 ***-1 for the clone RRIM 2020, and at 2,000 ***-1and 500 *** clone RRIM 2025. We conclude that the ideal planting density is 2,000 ***＂l for obtaining high volume of wood production and 500 trees-ha-1 for high wood quality.

关键词： clone planting density tree growth hevea brasiliensis

Evaluation of unconstrained and constrained mathematical functions to model girth growth of rubber trees (hevea brasiliensis) using young agemeasurements

在线全文

学校读者我要写书评

暂无评论

Journal of Forestry Research 2012年第3期23卷 365-375页

作者： T.R.Chandrasekhar Rubber Research Institute of India Hevea Breeding Sub StationKadaba-574221DK DistrictKarnatakaIndia

No attempt has been made to date to model growth in girth of rubber tree （hevea brasiliansis）. We evaluated the few widely used growth functions to identify the most parsimonious and biologically reasonable model for describing the girth growth of young rubber trees based on an incomplete set of young age measurements. Monthly data for girth of immature trees （age 2 to 12 yearsi from two locations were sub- jected to modelling. Re-parameterized, unconstrained and constrained growth functions,of Richards （RM）, Gompertz （GM） and the monomo- lecular ＇model ^（MM） were fitted to data. Duration of growth was the firsf constraint introduced. In the stagel We attempted a population aver- age （PA） model to capture the trend in growth. The best PA model was fitted as a subject specific （SS） model. We used appropriate error vari- ance-covariance structure to account for correlation due to repeated measurements over time. Unconstrainecl functions underestimated the asymptotic maximum that did not reflective carrying capacity of the locations. Underestimafions were attributed to the partial set＇ of meas- urements made during the early growth phase of the trees. MM proved superior to RM and GM. In the randomcoefficient models, both Gf and Go appeared to be influenced by tree level effects. Inclusion of diagonal definite positive matrix removed the correlation between random effects. The results were similar at both locations. In the overall assessment MM appeared as the candidate model for studying the girth-age relationships in hevea trees. Based on the fitted model we conclude that, in hevea trees, growth rate is maintained at maximum value at to, then decreases until the final state at dG/dt 〉 0, resulting in yield curve with no period of accelerating growth. One physiological explanation is that photosynthetic activity in hevea trees decreases as girth increases and constructive metabolism is larger than destructive metabolism.

关键词： natural rubber hevea brasiliensis growth modelling un-constrained functions constrained functions mixed model

Localized Effects of Mechanical Wounding and Exogenous Jasmonic Acid on the Induction of Secondary Laticifer Differentiation in Relation to the Distribution of Jasmonic Acid in hevea brasiliensis

在线全文

学校读者我要写书评

暂无评论

Acta Botanica Sinica 2003年第11期45卷 1366-1372页

作者：田维敏史敏晶于凤义吴继林郝秉中崔克明北京大学生命科学学院北京100871 华南热带农业大学农学院儋州571737 中国农业科学院原子能利用研究所北京100094 中国热带农业科学院橡胶栽培研究所热带作物栽培生理学农业部重点开放实验室儋州571737

The relationship between the effect of exogenous jasmonic acid (JA) on the induction of secondary laticifer differentiation and the distribution of JA in the seedling of hevea brasiliensis Mull. Arg. was investigated with the aid of experimental morphological and radioisotope technique. Most radioactivity of H-3-JA sustained in treated site within one hour while no radioactivity was detected in new shoot and the radioactivity in upper leaf was much less than that in the parts below the treated site, suggesting that JA was mainly transported downwards in the shoot of H brasiliensis. Mechanical wounding hindered the entrance of exogenous JA remarkably while held back the entered JA to the regions around wounded site. The effect of exogenous JA and mechanical wounding on the induction of the secondary laticifer differentiation was limited to treated site where high level of JA was expected. Mechanical wounding reduced the effect of exogenous JA on the differentiation of secondary laticifer, which could be ascribed to the hindrance of mechanical wounding to the entrance of exogenous JA. It was concluded from the combined data that a high accumulation of JA was required for inducing the secondary laticifer differentiation in H. brasiliensis.

关键词： hevea brasiliensis jasmonic acid mechanical wounding radioisotope technique laticifer differentiation

Fluctuation of Microfibrillar Protein Level in Lutoids of Primary Laticifers in Relation to the 67 kD Storage Protein in hevea brasiliensis

在线全文

学校读者我要写书评

暂无评论

Acta Botanica Sinica 2003年第2期45卷 127-130页

作者：田维敏韩亚琴吴继林胡正海西北大学植物研究所西安710069 中国热带农业科学院橡胶栽培研究所农业部热带作物栽培生理学重点开放实验室

Abundant microfibrillar protein inclusions were present in the lutoids of the primary laticifers in hevea brasiliensis Mull. Arg. Two forms of the inclusions could be distinguished under the electron microscope, each in separate lutoids. As revealed by SDS-PAGE, the 59.5 kD and 63.5 kD proteins were the major components of the microfibrillar protein purified by isoeleettic point precipitation. Western-blotting analysis indicated that they were immunorelated with the 67 kD protein accumulated in the protein-storing cells. The 59.5 kD and 63.5 kD proteins were abundant in the uppermost part, the stem of new shoot and sustained their abundance during the growth and development of new shoot while their contents decreased remarkably in the lower parts of the trunk, accompanying by the accumulation of 3-5 kinds of proteins with low molecular weights. This fluctuating pattern suggested that the degradation of the 59.5 kD and 63.5 kD proteins had nothing to do with the new shoot growth and may be closely related to the primary laticifer differentiation. The 67 kD protein could not be detected in the young stem of new shoot when its leaves were broze-colored while the protein started to be accumulated in the stem, when the leaves of new shoot had matured, behaving like a typical vegetative storage protein.

关键词： microfibrillar protein 67 kD protein shoot growth laticifer development hevea brasiliensis

Molecular Cloning and Bioinformatical Analysis of a cDNA Encoding Mitochondrial 50S Ribosomal Protein L21 from hevea brasiliensis Muell. Arg.

在线全文

学校读者我要写书评

暂无评论

Agricultural Science & Technology 2012年第6期13卷 1203-1206页

作者：邹智杨礼富中国热带农业科学院橡胶研究所/农业部橡胶树生物学与遗传资源利用重点实验室海南儋州571737

[Objective] ＂Tapping panel dryness （TPD）＂, a syndrome known as tapping incision blocked partly or entirely during latex exploiting, has become the most important factor causing great losses for rubber production. Aiming to elucidate the molecular mechanism of tapping panel dryness occurrence, this study carried out molecular cloning and bioinformatical analysis of a mRPL21 cDNA sequence, a gene associated with TPD. [Method] In a preliminary study, an expressed sequence tag （EST） encoding a deduced protein homologous to mitochondrial 50S ribosomal protein L21 （mRPL21）, which showed to be down-regulated in the latex of TPD-affected rubber trees, was isolated by suppression subtractive hybridization （SSH）. After ESTs assembling and RT-PCR validation, an 853 bp cDNA sequence with an open reading frame （ORF） was cloned, which was named as HbmRPL21 under GenBank accession number of HM230670. [Result] Bioinformatical analysis suggests that HbmRPL21 encodes a deduced polypeptide of 271 amino acids with a theoretical molecular weight （Mw） of 30.52 kDa and isolectric point （pI） of 8.40, and HbmRPL21 is a mitochondrion-targeted protein with a conserved domain of Ribosomal_L21p involving translation. Homology analysis reveals high amino acid sequence identity of mRPL21 from plants, while diversity of that between plant and animal kingdom. [Conclusion] This study laid the basis for further revealing the biological functions of mRPL21 in TPD-affected rubber trees.

关键词： hevea brasiliensis Tapping panel dryness syndrome Mitochondrial 50S ribosomal protein L21

Study on the Explant Disinfection of Root Culture of hevea brasiliensis

在线全文

学校读者我要写书评

暂无评论

Agricultural Science & Technology 2011年第4期12卷 514-516页

作者：姜泽海周权男李哲海南大学农学院海南儋州571737 中国热带农业科学院橡胶研究所国家橡胶树育种中心海南儋州571737

[Objective] The aim was to look for an effective way of decreasing explants contamination in the root tissue culture of hevea brasiliensis.[Method] In order to study root tissue culture of ***,new roots from forest section after treatment for one month were as explants,and 1 g/L carbendazim,75% ethanol,0.1% mercuric chloride and Yipeilong with different concentrations were used as disinfectants for roots disinfection.[Result] Before conventional disinfection,root explants were treated by 1 g/L carbendazim for 2.5 h,and disinfected by 75% ethanol for 30 s and 0.1% HgCl2 for 6 min,then cultured on the callus induction medium supplemented with 0.1% *** results showed that the contamination rate of explants decreased to 44.59%,and 25.60% explants survived after cultured for 30 d.[Conclusion] The study could provide theoretical foundation for the decrease of explant contamination in the root tissue culture of ***.

关键词： hevea brasiliensis Root explants Contamination control Carbendazim Yipeilong

Molecular Cloning and Sequence Analysis of a Tonoplast Aquaporin Gene TIP1 Associated with Tapping Panel Dryness in hevea brasiliensis

在线全文

学校读者我要写书评

暂无评论

Agricultural Biotechnology 2014年第3期3卷 14-17页

作者： Zhi ZOU Danhua WANG Yeyong MO Feng AN Lifu YANG Key Laboratory of Biology and Genetic Resources of Rubber Tree Ministry of Agriculture / Rubber Research InstituteChinese Academy of Tropical Agricultural Sciences

[Objective]To address the role of aquaporins（AQPs）in the occurrence of Tapping Panel Dryness（TPD）,a phenomenon with tapping incision blocked partly or entirely during latex exploiting in the rubber tree（hevea brasiliensis）that causes great losses on rubber production,a tonoplast aquaporin gene associated with TPD occurence was cloned and analyzed.[Method]Based on an EST down-regulated in TPD-affected rubber trees,a 774 bp cDNA designated HbTIP1 was isolated from the bark tissue of hevea brasiliensis with a combination of in silico cloning and *** the gene structure and sequence characteristics were analyzed using bioinformatics tools.[Result]The cDNA includes a 759 bp ORF,8 bp 5’UTR and 7 bp 3’*** analysis indicated that HbTIP1putatively encodes 252 amino acids with a theoretical molecular weight（Mw）of 25.88 kDa and isolectric point（pI）of *** analysis suggested that the deduced protein is predicted to have six transmembrane helices located to the vacuolar membrane and to harbor one conserved MIP domain that can be grouped into the tonoplast intrinsic proteins（TIPs）of aquaporin *** search revealed that the protein shares a similarity of more than 90%with the homologues in Theobroma cacao,Prunus persica,Citrus sinensis and Ricinus communis,supporting a highly conserved evolution.[Conclusion]This study provided basis for further revealing the regulatory role of AQPs in the TPD occurrence.

关键词： hevea brasiliensis Tapping panel dryness Aquaporin

Molecular Cloning and Sequence Analysis of an Aquaporin Gene Related to Tapping Panel Dryness in Rubber Tree( hevea brasiliensis Muell. Arg. )

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Biotechnology 2014年第2期3卷 37-39,43页

作者： Zhi ZOU Yeyong MO Dahua WANG Feng AN Lifu YANG Key Laboratory of Biology and Genetic Resources of Rubber Tree Ministry of Agriculture / Rubber Research InstituteChinese Academy of Tropical Agricultural Sciences

As a syndrome with tapping incision blocked partly or entirely during latex exploiting in rubber tree （hevea brasiliensis ）, ＂Tapping panel dryness （TPD）＂ causes great yield losses, thereby becoming the most important factor limiting rubber production. On the basis of an EST down-regulated in TPD-affected rubber trees, a 903 bp cDNA denoted HbPIP2;2 was isolated from the bark tissue with a combination of in silico cloning and RT-PCR. The cDNA contains an 867 hp ORF, 13 bp 5＇UTR and 23 bp 3＇ UTR. Sequence analysis indicated that HbPIP2;2 encodes 288 amino acids with a theoretical molecular weight （Mw） of 30. 71 kDa and isolectric point （Pi） of 8.20. Bioinformatics analysis suggested that the deduced protein is predicted to have six transmembrane helices located to the plasma membrane and harbor one conserved MIP domain that can be grouped into plasma membrane intrinsic proteins （PIPs） of aquaporin （AQP） family. Homolo- gy search revealed that the protein shares a similarity of more than 90% with the homologues in Ricinus communis, Popttlus trichocarpa, Juglans regia and Theobro- ma cacao, suooorting a *** conserved evolution. This study provided basis for further uncovering the regulatory role of AOPs in TPD occurrence.

关键词： hevea brasiliensis Tapping panel dryness Aquaporin Plasma membrane intrinsic protein