检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

hephaestin在脑铁代谢中的作用

生物物理学报 2009年第S1期25卷 119-120页

作者：常彦忠乔亚迢河北师范大学生命科学学院石家庄市裕华东路113号050016

膜铁转运辅助蛋白(hephaestin,Heph)是研究性连贫血(sex-linked anemic,sla)小鼠时发现的一种新的铁转运相关蛋白。它的表达有明显的组织特异性,小肠高度表达。

关键词： hephaestin brain Ceruloplasmin iron

在线全文

学校读者我要写书评

暂无评论

hephaestin在脑铁代谢中的作用

hephaestin在脑铁代谢中的作用

第十一次中国生物物理学术大会暨第九届全国会员代表大会

作者：常彦忠乔亚迢河北师范大学生命科学学院

＜正＞膜铁转运辅助蛋白（hephaestin,Heph）是研究性连贫血（sex-linked anemic,sla）小鼠时发现的一种新的铁转运相关蛋白。它的表达有明显的组织特异性,小肠高度表达,我

关键词： hephaestin brain Ceruloplasmin iron

Ferroportinl but not hephaestin was involved in the 6-hydroxydopamine induced iron accumulation in MES23.5 dopaminergic cells

在线全文

cnki会议

学校读者我要写书评

暂无评论

Ferroportinl but not hephaestin was involved in the 6-hydrox...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Ning SONG,Jun WANG,Hong JIANG,Jun-Xia XIE~* State Key Discipline:Physiology(in incubation),Department of Physiology,Qingdao University,Qingdao,266071

＜正＞Objective Iron accumulation in the substantia nigra was thought to play an important role in the pathogenesis of Parkinson’s disease（PD）.Our in vivo experiments suggested that iron transporters ferroportinl（FP1） and hephaestin （HP） might account for the nigral iron accumulation in 6-hydroxydopamine（6-OHDA） lesioned PD *** present study is to explore the mechanisms underlying FP1 and HP mis-regulation in MES23.5 dopaminergic *** Realtime PCR and western blots were applied to detect FP1,HP,ferritin and iron regulatory protein（IRP） *** interfering RNA was applied for gene *** cytometry was applied to determine iron induced oxidative stress. Results Both FP1 and HP were down-regulated,followed by the decreased iron efflux in 6-OHDA treated MES23.5 cells. FP1 showed a dose-dependent up-regulation while HP showed no response in MES23.5 cells overloaded with ferric ammonium citrate,indicating that down-regulation of FP1 and HP was not due to the increased intracellular iron content. In 6-OHDA treated cells,both IRP1 and IRP2 were up-regulated,and silencing of IRPs in MES23.5 cells dramatically abolished 6-OHDA-induced FP1 down-regulation and even reversed HP *** of FP1 but not HP would lead to the intracellular iron accumulation in MES23.5 *** Down-regulations of both FP1 and HP by 6-OHDA were ***1 but not HP was involved in the 6-OHDA induced iron accumulation in MES23.5 cells.

关键词： ferroportinl hephaestin 6-hydroxydopamine iron Parkinson’s disease

Overexpression of ferroportinl and/or hephaestin attenuated iron induced oxidative stress in MES23.5 cells

在线全文

cnki会议

学校读者我要写书评

暂无评论

Overexpression of ferroportinl and/or hephaestin attenuated ...

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Ning SONG,Jun WANG,Hong JIANG,Jun-Xia XIE~* Department of Physiology,Medical College of Qingdao University,Qingdao,266071

＜正＞Ferroportin1（FP1） and hephaestin（HP） are newly-found iron transporters cooperating in the iron efflux process in the *** their roles in brain iron homeostasis are not fully *** previous study demonstrated that decreased FP1 and HP expression were involved in the iron accumulation and dopaminergic neurons degeneration in Parkinson’s disease（PD） animal *** investigate whether overexpressed FP1 and/or HP could attenuate iron induced oxidative stress,in the present study we generated the stable MES23.5 dopaminergic cell lines with over expression of FP1 and/or HP,and the dopamine（DA） content and cellular oxidative stress under iron overloaded circumstances in control and transfected cells were *** results showed that iron reduced DA content in the control,however, a significantly less reduction of DA content was observed in transfected cells with high expression of FP1 and/or HP. Further investigations demonstrated that high expression of FP1 and/or HP could surpressed reactive oxygen species production and restored mitochondrial menbrane potential under iron overloaded conditions,due to more iron efflux from the *** findings suggest that overexpression of FP1 and/or HP can protect cells from iron induced oxidative stress by enhancing iron efflux process.

关键词： ferroportin1 hephaestin iron dopamine

大鼠急性脑缺血后hephaestin的表达变化

在线全文

cnki会议

学校读者我要写书评

暂无评论

河南实用神经疾病杂志 2004年第4期7卷 1-2页

作者：温仲民包仕尧王运良苏州大学附属第二医院神经内科苏州215004

目的研究大鼠急性局灶性脑缺血后皮质和纹状体hephaestin表达的变化。方法线栓法制备大鼠急性大脑中动脉阻塞(MCAO)再灌注模型,在再灌注后的不同时间点应用免疫组化、图像分析以及SDS-PAGE Werstern blot方法检测缺血侧皮质和纹状体... 详细信息

目的研究大鼠急性局灶性脑缺血后皮质和纹状体hephaestin表达的变化。方法线栓法制备大鼠急性大脑中动脉阻塞(MCAO)再灌注模型,在再灌注后的不同时间点应用免疫组化、图像分析以及SDS-PAGE Werstern blot方法检测缺血侧皮质和纹状体的表达变化。结果 MCAO再灌注后大鼠出现大脑中动脉梗死的神经系统损害体征,TTC染色有白色梗死区。hephaestin在正常大鼠的皮质、纹状体有表达,在急性脑缺血再灌注后12h缺血侧皮质、纹状体表达明显增加并持续到再灌注后48h,在24h时到达高峰(Phephaestin的表达出现明显的变化,在急性脑缺血的病理生理变化中可能起着重要的作用。

关键词：大鼠急性脑缺血 hephaestin 表达免疫组化图像分析

Sodium glutamate and gamma-aminobutyric acid affect iron metabolism in the rat caudate putamen

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2010年第21期5卷 1644-1649页

作者： Na Wang Peng Guan Fei Li Yujian Fu Xianglin Duan Yanzhong Chang Institute of Molecular Neurobiology and Neuropharmacology Hebei Normal University Shijiazhuang 050016 Hebei Province China Department of Basic Courses Hebei Medical University Shijiazhuang 050091 Hebei Province China

Glutamic acid and gamma-aminobutyric acid （GABA） influence iron content in the substantia nigra and globus pallidus, although the mechanisms of action remain unclear. The present study measured iron content and changes in divalent metal transporter 1 （DMT1） and hephaestin expression in the substantia nigra and caudate putamen, and explored the effects of GABA and glutamic acid on iron metabolism. Results demonstrated that iron content and DMT1 non iron response element [DMT1 （-IRE）] expression were significantly greater but hephaestin expression was significantly lower in the caudate putamen of the monosodium glutamate group compared with the control group. No significant difference in iron content was detected between the GABA and control groups. DMT1 （-IRE） expression was significantly reduced, but hephaestin expressiori was significantly increased in the GABA group compared with the control group. In addition, there was no significant difference in tyrosine hydroxylase expression between monosodium glutamate and GABA groups and the control group. These results suggested that glutamate affected iron metabolism in the caudate putamen by increasing DMTI（-IRE） and decreasing hephaestin expression. In addition, GABA decreased DMT1 （-IRE） expression in the caudate putamen.

关键词： glutamate gamma-aminobutyric acid tyrosine hydroxylase divalent metal transporter 1 non iron response element hephaestin

Quantitative Study of Iron Metabolism-related Genes Expression in Rat

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2013年第10期26卷 808-819页

作者： LI Yan Qin BAI Bin ZHENG Quan Qing YAN Hong ZHUANG Gui Hua Department of Public Health College of MedicineXi’an Jiaotong University Department of Orthopedics The First Affiliated Hospital of the College of MedicineXi’an Jiaotong University

Objective To investigate the multiple iron metabolism-related genes expression, its regulation by iron and the expression correlation among the genes in rat tissues. Methods Two groups （n=30） of Sprague-Dawley female weanling rats were fed with a control diet and an iron deficient diet respectively for 4 weeks. All rats were then sacrificed, and blood and tissue samples were collected. The routine blood examination was performed with a veterinary automatic blood cell analyzer. Elemental iron levels in liver, spleen and serum were determined by atomic absorption spectrophotometry. The mRNA expression of genes was detected by real-time fluorescence quantitative PCR. Results After 4 weeks, the hemoglobin （Hb） level and red blood cell （RBC） count were significantly lower in the iron deficient group compared with those in the control group. The iron levels in liver, spleen and serum in the iron deficient group were significantly lower than those in the control group. In reference to small intestine, the relative expression of each iron-related gene varied in the different tissues. Under the iron deficiency, the expression of these genes changed in a tissue-specific manner. The expression of most of the genes significantly correlated in intestine, spleen and lung, but few correlated in liver, heart and kidney. Conclusion Findings from our study provides new regulation by iron and correlation among the mRNA divalent metal transporter 1, ferritin, iron regulation protein, hepcidin, ferroportin 1 and hephaestin in intesti understandings about the relative expression, expressions of transferrin receptors 1 and 2, proteins 1 and 2, hereditary hemochromatosis ne, liver, spleen, kidney, heart, and lung of rat.

关键词： Iron Tfr Dmtl Hepcidin Ferroportin 1 hephaestin

Effects of Intranigral Injection of Glutamate/GABA on Iron Metabolism in the Nigrostriatum of Rats

在线全文

学校读者我要写书评

暂无评论

Effects of Intranigral Injection of Glutamate/GABA on Iron M...

第九次全国暨2007海内外生物膜学术研讨会

作者： Peng Guan~(1 2) Xiang-Lin Duan~(1 2) Na Wang~(1 2) Yan-Zhong Chang~(1 2) 1 The Institute of Molecular Neurobiology and Neuropharmacology Hebei Normal University Shijiazhuang 050016 Hebei Province China 2 The key labortry of Animal Physiology Biochemistry and Molecular Biology of Hebei Province Hebei Normal University Shijiazhuang 050016 Hebei Province China

＜正＞To investigate the effects of intranigral increased glutamate/GABA on iron metabolism in the nigrostriatum of rats,Glutamate Mono-Sodium（GMS）and GABA was respectively stereotaxically injected into substantia n... 详细信息

关键词： Glutamate GABA DMTI(-IRE) hephaestin

6-OHDA单侧损毁大鼠黑质铁转出蛋白的表达降低

在线全文

cnki会议

学校读者我要写书评

暂无评论

6-OHDA单侧损毁大鼠黑质铁转出蛋白的表达降低

中国神经科学学会第六届学术会议暨学会成立十周年庆祝大会

作者：王俊姜宏谢俊霞青岛大学医学院青岛大学医学院青岛大学医学院

＜正＞目的:黑质（SN）铁聚积被认为参与了帕金森病（PD）的发病,而导致脑铁增高的原因目前仍不清楚,可能是由于某些铁转运蛋白的表达失控所致。Ferroportinl（FP1）和hephaestin（HP）是新近发现的跨膜铁转出蛋白,在小肠中相互作用共... 详细信息

＜正＞目的:黑质（SN）铁聚积被认为参与了帕金森病（PD）的发病,而导致脑铁增高的原因目前仍不清楚,可能是由于某些铁转运蛋白的表达失控所致。Ferroportinl（FP1）和hephaestin（HP）是新近发现的跨膜铁转出蛋白,在小肠中相互作用共同完成铁的转出功能。目前国际、国内关于脑铁代谢机制的认识只是初步的,尤其是对脑铁转出的机制知之甚少,尚未见有关FP1和HP参与脑铁转出的报道。本实验旨在观察FP1和HP在大鼠脑内是否共存,其表达异常是否引起了PD SN

关键词： 6-羟基多巴胺 ferroportinl hephaestin 大鼠铁