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Presence of hepcidin-25 in biological fluids: Bile,ascitic and pleural fluids

World Journal of Gastroenterology 2010年第17期16卷 2129-2133页

作者： Jayantha Arnold Arvind Sangwaiya Vijay Manglam Frank Geoghegan Mark Thursz Mark Busbridge Department of Gastroenterology Ealing Hospital NHS TrustUxbridge RoadLondon UB1 3HWUnited Kingdom Department of Biochemistry Ealing Hospital NHS TrustUxbridge RoadLondon UB1 3HWUnited Kingdom Department of Hepatology St Mary's Hospital NHS TrustImperial CollegePraed StreetLondon W2 1NYUnited Kingdom Department of Clinical Chemistry Charing Cross and Hammersmith Hospital NHS TrustImperial CollegeFulham Palace RoadLondon W6 8RFUnited Kingdom

AIM: To examine body fluids such as ascitic fluid (AF),saliva,bile and pleural effusions for the presence of hepcidin using a novel radioimmunoassay (RIA).METHODS: Serum samples were collected from 25 healthy volunteers (mean age: 36 ± 11.9 years,11 males,14 females).In addition bile was obtained from 12 patients undergoing endoscopic retrograde cholangiopancreatography (mean age: 66.9 ± 16.7 years,M:F = 5:7).Saliva was collected from 17 healthy volunteers (mean age: 35 ± 9.9 years,M:F = 8:9).Pleural and AF were collected from 11 and 16 patients [(mean age: 72 ± 20.5 years,M:F = 7:4) and (mean age: 67.32 ± 15.2 years,M:F = 12:4)],*** biological fluid samples (serum,exudative and transudative fluids) were tested for the presence of hepcidin-25 molecule using ***: hepcidin-25 was detected in all biological fluids *** mean ± SD hepcidin-25 in serum was 15.68 ± 15.7 ng/mL,bile 7.37 ± 7.4 ng/mL,saliva 3.4 ± 2.8 ng/mL,exudative fluid 65.64 ± 96.82 ng/mL and transudative fluid 14.1 ± 17.8 ng/***: We provide clear evidence that hepcidin-25 is present in bile,saliva,pleural and ascitic *** is likely to play a role here in innate immunity.

关键词： hepcidin hepcidin assay hepcidin in biological fluids hepcidin in ascitic fluid Bile Exudates Antimicrobial peptides

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hepcidin levels in hereditary hyperferritinemia:Insights into the iron-sensing mechanism in hepatocytes

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World Journal of Gastroenterology 2010年第28期16卷 3541-3545页

作者： Jayantha Arnold Arvind Sangwaiya Vijay Manglam Mark Thursz Caroline Beaumont Caroline Kannengiesser Mark Busbridge Department of Gastroenterology Ealing HospitalLondonUB1 3HWUnited Kingdom Department of Hepatology St Mary's Hospital Imperial College LondonLondonW2 1NYUnited Kingdom Biochemical hormones and Genetics Laboratory Bichat Claude Bernard Hospital46Rue Henri HuchardParis75018France Clinical Chemistry Hammersmith Hospital Imperial College LondonLondonW6 8RFUnited Kingdom

AIM:To study the role of hepcidin in hereditary hyperferritinemia cataract syndrome(HHCS). METHODS:Six patients from two families with HHCS, confirmed by genetic analysis showing A to G mutation at position+40 in the L-ferritin gene,were recruited to undergo serum hepcidin and prohepcidin measurements using radioimmunoassay and enzyme linked immunoassay,respectively,and measurements were compared with levels in serum from 25 healthy volunteers(14 females),mean age 36±11.9 ***:The serum hepcidin and prohepcidin levels in patients with HHCS were 19.1±18.6 and 187± 120.9 ng/mL,*** ferritin was 1716.3± 376μg/*** biopsy in one patient did not show any evidence of iron *** hepcidin and prohepcidin values in healthy controls(HCs)were 15.30±15.71 and 236.88±83.68 ng/mL,respectively,while serum ferritin was 110±128.08μg/*** was no statistical difference in serum hepcidin level between the two cohorts(19.1±18.6 ng/mL vs 15.30±15.71 ng/mL,P= 0.612)using two-tailed t-test. CONCLUSION:Serum hepcidin levels in HHCS patients is similar to that in *** study suggests that circulating ferritin is not a factor influencing hepcidin synthesis and does not have a role in the iron-sensing mechanism in hepatocytes.

关键词： Hereditary hyperferritinemia Hereditary hyperferritinemia cataract syndrome hepcidin hepcidin assay Iron-sensing mechanism Iron responsive element Ferritin

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