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clinical application and analysis of hepatitis c virus NS3 antigen detection by ELISA in human serum

chinese Medical Journal 2007年第4期120卷 294-299页

作者： XIE Li WU Xiao-dong HUANG De-zhuang cHEN Hai-lun HE Li-xiang WANG Jian HAN Da-kang Institute for Viral hepatitis center for clinical Laboratory Artificial Liver center Beijing You＇an Hospital Capital Medical University Beijing100069 China Department of Biology Graduate University of Chinese Academyof Sciences Beijing 100049 China Department of Microbiology Capital Medical University Beijing100069 China

Background hepatitis c virus （HcV） core antigen assays have been produced to exclude infectious donations collected during the preseroconversion window phase （PWP）. For the same purpose, we evaluated the specificity and sensitivity of a novel hepatitis c virus NS3 antigen detection immunoassay and the application of this assay in clinical diagnosis. Methods Samples from 77 healthy subjects, 173 anti-HcV positive patients and 3708 hepatitis patients other than HcV positive were tested with the HcV NS3 antigen assay. Some HcV NS3 antigen positive samples were further validated with HcV-rna, neutralization and immunodot assays. Twenty-five sequential samples from 11 HcV NS3 antigen positive patients were subjected to kinetic study. Results Only 48 （1.3%） of 3708 anti-HcV negative samples were positive for HcV NS3 antigen. Among them, 44 of 3030 samples from patients only infected with HBV were HcV NS3 antigen positive, 4 of the 445 samples from patients infected with other type hepatitis were HcV NS3 antigen positive. In addition, 42 （24.3%） of 173 anti-HcV positive samples were HcV NS3 antigen positive and all 77 samples from healthy subjects were negative to HcV NS3 antigen assay. Of the 15 HcV NS3 antigen positive samples, 9 （60%） were HcV-rna positive. The neutralization and positive percentage of immunodot assay for 23 HcV NS3 antigen positive sera were 87.0% （20/23） and 69.6% （16/23） respectively. Of the 25 sequential samples from 11 HcV NS3 antigen positive patients, there was a negative correlation between the OD values and the duration of test （r=-0.989, P〈0.05）, and there were correlations among their HcV NS3 antigen, HcV-rna and anti-HcV Utres. The anti-HcV antibodies of two sera were detected while their OD values of HcV NS3 antigen decreased gradually. conclusions The HcV NS3 antigen detection assay showed perfect specificity and high sensitivity. Thus, it would be useful and economical as a routine test in laboratories for early diagnosis of HcV infection and prevention.

关键词： hepatitis c virus hepatitis c virus NS3 antigen hepatitis c virus rna anti-HcV,＂ ELISA

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hepatitis c virus micro-elimination:Where do we stand?

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World Journal of Gastroenterology 2021年第16期27卷 1728-1737页

作者： Alessandra Mangia Rosa cotugno Giovanna cocomazzi Maria Maddalena Squillante Valeria Piazzolla Liver Unit Department of Medical SciencesIRCCS Casa Sollievo della SofferenzaSan Giovanni Rotondo 71013FgItaly

hepatitis c virus (HcV) elimination by 2030, using direct-acting antiviraltreatments, has been promoted by the World Health Organization. Thisachievement is not attainable, however, particularly after the 2020 pandemic ofthe coronavirus disease 2019. consequently, the more realistic objective ofeliminating HcV from population segments for which targeted strategies ofprevention and treatment are easily attained has been promoted in Europe, as avalid alternative. The underlying idea is that micro-elimination will ultimatelylead to macro-elimination. The micro-elimination strategy may target differentspecific populations and at-risk groups. Different settings, including prisons andhospitals, have also been identified as micro-elimination scenarios. In addition,dedicated micro-elimination strategies have been designed that are tailored at thegeographical level according to HcV epidemiology and individual country’sincome. The main elements of a valid and successful micro-elimination project arereliable epidemiological data and active involvement of all the stakeholders.community involvement represents another essential component for a successfulprogram.

关键词： hepatitis c virus antibodies hepatitis c virus elimination hepatitis c virus epidemiology hepatitis c virus rna hepatitis c virus diagnosis hepatitis c virus infection

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Detection　of　Replicative　Form　of　HcV　rna　in　Peripheral　Blood　Leukocytes　and　Its　clinical　Significance

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Journal of Huazhong University of Science and Technology(Medical Sciences) 1995年第3期15卷 133-137页

作者：贺永文罗端德 Department of Infectious Diseases Xiehe Hospital Tongji Medical University Wuhan Department of Immunology University Hospital of Essen Federal Republic of Germany

Nested RT-PcR, done by using degenerated primer pair, was used to detect hepatitis c virus rna (HcV rna) in serum, plasma, liver and peripheral blood mononuclear cells (PBMc) of 30 patients with acute and chronic posttransfusion hepatitis c and 7 asymptomatic anti-HcV positive subjects. The results showed that the percentages of both the plus and minus strands of HcV rna in PBMc of the patients with chronic hepatitis c was significantly higher than that with acute hepatitis c and asymptomatic anti-HcV positive subjects ( Pcted to biopsy, the positive rate of the both strands of HcV rna in PBMc of the patients with AH was lower than that of cAH ( Pcases, the minus strand of HcV rna was not detected. Both plus and minus strands in liver of one patient with AH were positive , but the minus strand in PBMc negative. In 6 patients with cAH whose both strands in liver were positive,Both strands in PBMc in 5 patients were also found. The present data confirmed that PBMc of the patients with hepatitis c were infected by HcV and the longer the infection time, the bigger the possibility of PBMc infection by HcV. The patients with active liver disease (cAH) had higher positive rate of minus strands of HcV rna in PBMc. The results suggested that HcV may not only infect PBMc but also replicate in PBMc , and that the occurrence of minus strand of HcV rna is associated with activity of liver disease.

关键词： hepatitis c virus rna nested RT-PcR peripheral blood mononuclear cells

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