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Virologic characteristics of hepatitis b virus in patients infected via maternal-fetal transmission

World Journal of Gastroenterology 2008年第37期14卷 5674-5682页

作者： Tao Shen Xin-Min Yan Yun-Lian Zou Jian-Mei Gao Hong Dong The Institute of basic Medicine of the First People'sHospital of Yunnan Province Kunrning 650032YunnanProvince China

AIM: To determine whether HbV with the same characteristics causes dissimilar mutations in different hosts. METHODS: Full-length HbV genome was amplified and linked with pMD T18 vector. Positive clones were selected by double-restriction endonuclease digestion (EcoRⅠ and HindⅢ) and PCR. Twenty seven clones were randomly selected from an asymptomatic mother [at two time points: 602 (1 d) and 6022 (6 mo)] and her son [602 (S)], and the phylogenetic and mutational analysis was performed using bioEditor, Clustal X and MEGA software. Potential immune epitopes were determined by the Stabilized Matrix Method (SMM), SMM-Align Method and Emini Surface Accessibility Prediction. RESULTS: All of the 27 sequences were genotype C, the divergence between the mother and son was 0%-0.8%. Compared with another 50 complete sequences of genotype C, the mother and her son each had 13 specific nucleotides that differed from the other genotype C isolates. AA 1-11 deletion in preS1 was the dominant mutation in the mother (14/18). The 1762T/1764A double mutation existed in all clones of the mother, 3 of them were also coupled with G1896A mutation, but none were found in the son.17 bp deletion starting at nucleotide 2330 was the major mutation (5/9) in the son, which caused seven potential HLA class Ⅰ epitopes and one b cell epitope deletion, and produced a presumptive new start codon, downstream from the original one of the P gene. CONCLUSION: The HbV strain in the son came from his mother, and discrepant mutation occurred in the mother and her son during infection.

关键词： hepatic b virus Vertical transmission Fullgenome Mutation Phylogenetic Deletion

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Hepatocellular carcinoma in patients with autoimmune hepatitis

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World Journal of Gastroenterology 2009年第5期15卷 578-582页

作者： Andreas Teufel Arndt Weinmann Catherine Centner Anja Piendl Ansgar W Lohse Peter R Galle Stephan Kanzler Department of Medicine I Johannes Gutenberg University Mainz 55101 Germany Department of Medicine I University of Hamburg Hamburg 22527 Germany

AIM: To evaluate and confirm the low incidence of hepatocellular carcinoma (HCC) in patients with autoimmune hepatitis (AIH). At present only very few cases of HCC in patients with AIH and definite exclusion of chronic viral hepatitis have been published, suggesting that HCC due to AIH is rare. METHODS: In order to further investigate the incidence of HCC in patients with AIH, we reviewed our large cohort of 278 patients with AIH. RESULTS: Eighty-nine patients (32%) were diagnosed with liver cirrhosis, a preneoplastic condition for HCC. We studied a total of 431 patient years of cirrhosis in these patients, an average 4.8 years per patient. During this period none of the patients of our own study cohort developed HCC. However, three patients with HCC due to AIH associated liver cirrhosis were referred to our department for further treatment of HCC. In all three patients chronic viral hepatitis was excluded. CONCLUSION: We conclude that HCC may under rare circumstances develop due to chronic AIH dependent liver cirrhosis. Compared to other causes of liver cirrhosis such as chronic viral hepatitis, alcohol, or hemochromatosis, the incidence of HCC is significantly lower. Pathophysiological differences between AIH and chronic viral hepatitis responsible for differences in the incidence of HCC are yet to be further characterized and may lead to new therapeutic concepts in prevention and treatment of liver cancer.

关键词： Autoimmune hepatitis Hepatocellularcarcinoma hepatic C virus hepatic b virus Livercirrhosis

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