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Effect of Artemisia annua (Asteraceae) Extracts on hemolysis in Individuals with G6PD-Deficiency

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Journal of Biosciences and Medicines 2024年第3期12卷 303-315页

作者： Jescica Bonarck Sohou Casimir Jiras Kpego Ligan Salomon Ezechiel Mahougnon Fiogbe Thierry Comlan Marc Medehouenou Fifame Esperance Elvire Kougnimon Casimir Dewanou Akpovi Unité de Recherche sur les Maladies Non Transmissibles et le Cancer (UR-MNTC) Laboratoire de Recherche en Biologie Appliquée Ecole Polytechnique d’Abomey-Calavi University of Abomey-Calavi Cotonou Benin

Individuals with Glucose-6-phosphate dehydrogenase (G6PD) deficiency are susceptible to hemolytic anemia when exposed to pro-oxidant substances. This study investigates the hemolytic impact of Artemisia annua (A. annua) extracts in G6PD-deficient subjects through a mixed experimental approach. In the in vitro phase, red blood cells from G6PD-deficient individuals and rats induced with Dehydroepiandrosterone (DHEA) were exposed to various concentrations of A. annua infusion, with distilled water and physiological saline as positive and negative controls respectively. The in vivo study involved G6PD-deficient Wistar rats divided into three groups receiving A. annua infusion, quinine (positive control), and distilled water (negative control) via gavage. Blood samples were collected for biochemical and hematological analyses. Notably, at a 40% concentration of A. annua infusion, there was a significant increase in the hemolysis rate of G6PD-deficient red blood cells compared to controls (p A. annua exhibited elevated aspartate aminotransferase (129.25 ± 4.55 U/L vs. 80.09 ± 4.03 U/L;p A. annua infusion tested positive for saponins. These findings underscore the risk of hemolysis in G6PD-deficient individuals upon ingesting A. annua.

关键词： Artemisia annua Infusion Saponins G6PD Deficiency hemolysis

Identification,Characterization,and Probiotic Potentials of Lactobacillus pentosus SF-1

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Journal of Ocean University of China 2024年第2期23卷 509-517页

作者： JIAO Lin LIANG Shufei CHU Ruixia WANG Wentao MAI Kangsen LIU Ming WAN Min Key Laboratory of Aquaculture Nutrition and Feed Ministry of Agriculture&Key Laboratory of MaricultureMinistry of EducationCollege of FisheriesOcean University of ChinaQingdao 266003China Engineering Research Center of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China

In recent years probiotics have been considered as a potential substitution of antibiotics to control pathogens and treat infectious diseases in aquaculture.In the present study a strain of Lactobacillus pentosus,named as L.pentosus SF-1,was isolated from waters in aquaculture.The species identification of this strain was conducted by 16S rRNA sequence,and the physiological and biochemical characteristics of this strain were assessed.Furthermore,the virulence,antibiotic sensitivity,cell surface characteristics and acid/base-resistance of L.pentosus SF-1 were determined to evaluate the probiotic potentials of this strain.Specifically,L.pentosus SF-1 is sensitive to most common antibiotics,and no hemolysin was generated from it,indicating the safety of this strain to hosts.In addition,L.pentosus SF-1 was able to tolerate the artificial gastric juice at pH 3 for 4 h and the artificial intestinal fluid at pH 6.8 or 8.0 for 6 h.Moreover,the analysis of self-aggregation and the adhesion of L.pentosus SF-1 to organic solvents suggested a high potential of L.pentosus SF-1 to inhabit the hosts,which was confirmed by testing the colonization of L.pentosus SF-1 in germ-free zebrafish.Interestingly,L.pentosus SF-1 displayed a high bactericidal activity against several bacterial pathogens.Consistently,the incubation of L.pentosus SF-1 significantly promoted the expression of antimicrobial components in zebrafish,contributing to the protection of the fish from E.tarda infection in vivo.Taken together,the probiotic strain L.pentosus SF-1 could be applied as anti-infection reagent in aquaculture.

关键词： Lactobacillus pentosus probiotics antibiotics hemolysis cell surface characteristics bactericidal activity infection

hemolysis of PM_(10) on RBCs in vitro:An indoor air study in a coal-burning lung cancer epidemic area

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Geoscience Frontiers 2022年第1期13卷 506-511页

作者： Mengyuan Zhang Longyi Shao Tim Jones Ying Hu Rachel Adams Kelly BéruBé State Key Laboratory of Coal Resources and Safe Mining and College of Geoscience and Surveying EngineeringChina University of Mining&TechnologyBeijing 100083China School of Earth and Environmental Sciences Cardiff UniversityMuseum AvenueCardiff CF103YEUK Guangdong Province Nuclear Industry Geological Bureau Guangzhou 510800China School of Health Sciences Cardiff Metropolitan UniversityLlandaff CampusWestern AvenueCardiff CF52YBWalesUK School of Biosciences Cardiff UniversityMuseum AvenueCardiff CF103AXWalesUK

Epidemiological studies have suggested that inhalation exposure to indoor ambient air from coal-burning environments is causally associated with respiratory health risks.In order to explore the toxicological mechanisms behind the adverse health effects,the hemolytic activity of PM_(10)(particulate matter with an aerodynamic diameter of 10 um or less)samples collected from homes burning coal in the recognized China"cancer village"Xuanwei were evaluated and matched against their trace elemental contents.The results demonstrated that the hemolytic activity of indoor PM_(10) in coal-burning environments ranged from 4.28%to 5.24%,with a clear positive dose-response relationship.Although low dose samples exhibited a reduced hemolytic activity,PM_(10) could have a toxic effect upon people in a coal-burning indoor environment for extended time periods.The concentrations of analyzed trace elements in PM_(10) samples ranged from 6966 to 12,958 ppm.Among the analyzed elements,Zn,Ti,Ni,Cu,Pb,Ba,Mn,Cr and V were found at higher concentrations and accounted for over 95%of the total elements.The concentrations of total analyzed elements in the PM_(10) samples revealed a significant positive correlation with PM_(10) hemolytic activity.Of the analyzed elements,Zn,Pb and Cs positively correlated with hemolysis,while Li,U and V negatively correlated with the hemolysis of human red blood cells(RBCs).Therefore,the heavy metal elements could be one of the main factors responsible for the hemolytic capacity of indoor PM_(10) in coal-burning environments.

关键词： Air pollution PM_(10) Coal hemolysis Xuanwei County

Inhibition of Monosodium Urate Monohydrate-mediated hemolysis by Vitamin E

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Acta Biochimica et Biophysica Sinica 2007年第4期39卷 273-277页

作者： Qiong XIE1# Shude LI~2# Welyang FENG~2 Yongzhi LI~1 Yuanliang WU~1 Wei HU~1 Youguang HUANG~2* 1 Institate of Space Medico-Engineering,Beijing 100094,China 2 Department of Biochemistry Kunming Medical College,Kunming 650031,China Institute of Space Medico-EngineeringBeijing 100094 China Department of Biochemistry Kunming Medical CollegeKunming 650031 China

Microcrystals of monosodium urate monohydrate(MSUM)induce cytolysis and hemolysis inerythrocytes.In this report,we studied the effect of vitamin E on MSUM-mediated hemolysis in humanerythrocytes.Vitamin E significantly inhibited hemolysis induced by MSUM.The hydroxyl group in thechromanol ring of vitamin E is dispensable for protecting erythrocytes against hemolysis induced by MSUM,indicating that the inhibitory effect of vitamin E is not due to its antioxidant properties.However,both thechromanol ring and the isoprenoid side chain are important for vitamin E to suppress MSUM-induced hemolysis.Our current study suggests that vitamin E inhibits hemolysis induced by MSUM as a membrane stabilizer.

关键词： monosodium urate monohydrate erythrocyte hemolysis vitamin E membrane stabilizer

Cytocompatibility and hemolysis of AZ31B Magnesium Alloy with Si-containing Coating

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Journal of Materials Science & Technology 2015年第8期31卷 845-851页

作者： Qiang Wang Lili Tan Ke Yang School of Stomatology China Medical University Institute of Metal Research Chinese Academy of Sciences

In the present study, a Si-containing coating was fabricated on AZ31B Mg alloy. Cytocompatibility of the coated alloy was evaluated by both indirect and direct contact methods, respectively. Effects of a number of incubation variables on the sensitivity and reproducibility of the hemolysis test were also examined by using positively and negatively responding biomaterials. Cytocompatibility testing results indicated that cell condition, cell adherence, cell proliferation and extracellular matrix secretion of the coated alloy were improved compared with those of the uncoated alloy for different extraction and co-culture time. The hemolysis test suggested that hemolysis testing conditions were critical to determine the hemolysis of the alloy. It was also found that 1 day in vitro degradation of the uncoated AZ31B alloy had no destructive effect on erythrocyte. As for the coated AZ31B alloy at any time point, the hemolysis rate was much lower than 5%, the safe value for biomaterials. These in vitro experimental results indicate that the Si-containing coating is effective to improve the cytocompatibility and hemolysis behaviors of AZ31B alloy during its degradation.

关键词： Magnesium alloys Biodegradable Cytocompatibility hemolysis

Fatal hemolysis due to clostridium perfrigens blood stream infection

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Chinese Medical Journal 2013年第18期126卷 3572-3573页

作者： CAO Bin SU Ling-ling LI Bin-bin LIU Ying-mei Department of Infectious Diseases and Clinical Microbiology Beijing Chao-Yang Hospital Capital Medical University Beijing Institute of Respiratory Medicine Beijing 100020 China

Here we reported a 59-year-old woman with diabetes who was found to have massive hemolysis associatedwith clostridium （C.） perfringens blood stream infection. She died 13 hours after admission in spite of aggressive treatment. The lady referred to our hospital with high fever, chills, body ache and right hypochondriac pain for 1 day. Physical examination revealed a temperature of 39, heart rate of 110 beats/min, blood pressure of 101/69 mmHg, and a respiratory rate of 25 breaths/min. Pulse oximetry was 94% on room air. Chest auscultation revealed good air movement without rales. Tenderness of right upper abdomen was found. Abnormal laboratory results included white blood cell （WBC） count of 20x109/L with 90.9% neutrophils,

关键词： hemolysis sepsis blood stream infection bacteremia clostridium perfringens

Correlation between microbubble-induced acoustic cavitation and hemolysis in vitro

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Chinese Physics B 2011年第2期20卷 316-320页

作者：张春兵刘政郭霞生章东 The Traditional Chinese Medicine Hospital of Jiangsu Province Institute of Acoustics Key Laboratory of Modern Acoustics (Nanjing University)Ministry of EducationNanjing University Xinqiao Hospital Third Military Medical University

Microbubbles promise to enhance the efficiency of ultrasound-mediated drug delivery and gene therapy by taking advantage of artificial cavitation nuclei. The purpose of this study is to examine the ultrasound-induced hemolysis in the application of drug delivery in the presence of microbubbles. To achieve this goal, human red blood cells mixed with microbubbles were exposed to 1-MHz pulsed ultrasound. The hemolysis level was measured by a flow cytometry, and the cavitation dose was detected by a passive cavitation detecting system. The results demonstrate that larger cavitation dose would be generated with the increase of acoustic pressure, which might give rise to the enhancement of hemolysis. Besides the experimental observations, the acoustic pressure dependence of the radial oscillation of microbubble was theoretically estimated. The comparison between the experimental and calculation results indicates that the hemolysis should be highly correlated to the acoustic cavitation.

关键词： acoustic cavitation passive cavitation detection microbubbles hemolysis

Left ventricular assist device hemolysis leading to dysphagia

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World Journal of Gastroenterology 2015年第18期21卷 5735-5738页

作者： Alexander Wuschek Sara Iqbal Jerry Estep Eamonn Quigley David Richards Department of Medicine Division of Gastroenterology Houston Methodist Hospital Houston TX 77030 United States

A 41-year-old man with a continuous- flow left ventricular assist device presented for evaluation of dysphagia and dark urine. He was found to have a significantly elevated L-lactate dehydrogenaseand an elevated plasma free hemoglobin consistent with intravascular hemolysis.After the hemolysis ceased,both the black urine and dysphagia resolved spontaneously.Transient esophageal dysfunction,as a manifestation of gastrointestinal dysmotility,is known to occur in the setting of hemolysis.Paroxysmal nocturnal hemoglobinuria is another recognized cause of massive hemolysis with gastrointestinal dysmotility occurring in25%-35%of patients during a paroxysm.Intravascular hemolysis increases plasma free hemoglobin,which scavenges nitric oxide(NO),an important second messenger for smooth muscle cell relaxation.The decrease in NO can lead to esophageal spasm and resultant dysphagia.In our patient the resolution of hemolysis resulted in resolution of dysphagia.

关键词： hemolysis dysphagia Esophageal spasm Nitric oxide Left ventricular assist device

Antioxidative Effect of Schiff Bases with o-Hydroxybenzylidene Groupon Free Radical Induced hemolysis of Human Red Blood Cell

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Chemical Research in Chinese Universities 2002年第3期18卷 287-289页

作者： LUO Xu yang ZHAO Jian zhang LIN Ying jie LIU Zai qun College of Chemistry Jilin UniversityChangchun 130023P.R.China

The hemolysis of human red blood cells was initiated by a water soluble free radical initiator, 2,2′ azobis (2 amidinopropane hydrochloride)(AAPH), at 37 ℃ in phosphate buffered saline(pH=7.4). The respective addition of 1 [ N (o hydroxybenzylidene)amino]tetradecane(TDCA), 1,2 di[ N (o hydroxybenzylidene)amino]cyclohexane(DACH), 4 [ N (o hydroxybenzylidene)amino]benzoic acid(PABA), 4 nitro[ N (o hydroxybenzylidene)]aniline(APNA) or N (o hydroxybenzylidene)aniline(APA) can all prolong the inhibition period of hemolysis, indicating that the above Schiff bases play an antioxidative role in free radical induced hemolysis. It can be concluded that Schiff base with an alkyl group or a conjugated system in the molecule protect red blood cells against free radical induced hemolysis efficiently. This information may be useful for antioxidant drug design.

关键词： Red blood cell hemolysis Schiff base Free radical