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Macro and Micromorphological Studies on Seven Species of heliotropiumL. (Boraginaceae Juss.) in South West of Saudi Arabia

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American Journal of Plant Sciences 2015年第9期6卷 1370-1377页

作者： Wael Taha Kasem Botany and Microbiology Department Faculty of Science Al-Azhar University Cairo Egypt Biology Department Faculty of Science Jazan University Jazan KSA

Genus heliotropium L. (Heliotropiaceae) in south west of Saudi Arabia has been studied;seven species of H. arbainense Fresen, H. longiflorum (D.C.) Jauber & Spach, H. petrocarpum Hockst & Steud, H. strigosium Willd, H. zeylanicum Lam., H. jizanense Al-Turki and H. lasiocarpum Fisch were collected, recognized, typed and nomenclatured. The most valuable characters were those of macro and distinct micromorphological data such as stomata, hairs, pollen grains and stem anatomy. Light microscopy has been used in these studies. From the obtained results, trichomes and pollen grains data in addition to stem anatomy indicated good taxonomic tools to differentiate between species of this genus. Different traditional measurements were strikingly also helpful for the discrimination of species. H. longiflorum was characterized by distinctive data such as prominent anatomical information, P/E (1.8 μm) in addition to presence of spindle hair which in turn can be separated as a taxonomic level. Also, the results revealed a complete affinity between the two species of H. jizanense and H. lasiocarpum. An artificial key is provided for the differentiation between the studied species.

关键词： heliotropium L. Leaf Venation Stomata Trichomes Pollen Grains Stem Anatomy

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Highly Efficient in VitroRegeneration Method of Endangered Medicinal Plant heliotropium kotschyi(Ramram) in the Kingdom of Bahrain

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American Journal of Plant Sciences 2014年第5期5卷 736-747页

作者： Manal Ahmed Sadeq Malabika Roy Pathak Ahmed Ali Salih Mohammed Abido Asma Abahussain Plant Tissue Culture Laboratory Desert and Arid Zone Sciences Program College of Graduate Studies Arabian Gulf University Manama Kingdom of Bahrain

heliotropium kotschyi (Ramram), is a very important plant in relation to environment protection and for its medicinal value in Bahrain. It has been detected as one of the endangered plants in Bahrain. Ex situ conservation of this plant applying tissue culture method was used in the present study. In vitro plant regeneration has been established via shoot organogenesis using nodal meristems. The effect of various plant growth regulators was evaluated to regenerate the whole plants by in vitro micropropagation in modified MS media supplemented with different plant growth regulators (PGRs). In vitro regeneration of plants was involved in three steps by shoot initiation, multiplication and rooting of microshoots. Initially 100% explants of heliotropium kotschyi responded to initiate shoot in MS medium supplemented with 8.88 μM BAP with 5.71 μM IAA after 4 weeks of culture. Multiplication of shoots and ultimate plant regeneration capacity of heliotropium kotschyi were compared and the highest performance of 578 shoots/explant was observed in presence of 8.88 μM BAP and 5.71 μM IAA after second transfer. Rooted plantlets were transferred to soil pots and 60% plants were survived after one and a half months. The highly efficient plant regeneration protocol of the desert plant is of great value in rapid plant propagation program for the purpose of conservation of biodiversity.

关键词： Ex Situ Conservation heliotropium kotschyi Organogenesis Plant Regeneration

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