检索结果-南通市图书馆

Immune responses to the expressed products of the CSP antigen gene of Plasmondium falciparum south in China isolate FCC1/HN in Hela cell

引用

南通大学学报(医学版) 2003年第1期 115-115页

作者： Yu XB Liu YW Luo SH

Objective:To construct a eukaryotic expression system with pcDNA3-PfCSP/Hela for the Circumsporozoite protein(CSP) gene of Plasmodium falciparum(***),to observe the immune responses in BALB/c mice induced by the expressed proteins. Methods:The recombinant plasmid pcDNA3-PfCSP was transformed into the Hela cell *** expressed protein was isolated and analyzed by using SIDS-PAGE and used for immunization of BALB/c mice by subcutaneous, intravenous, and intraperitoneal ***-linked immunosorbent assay(ELISA),Dot-ELISA,Western blot,T lymphocyte proliferation test,natural killer cell(NKC) activity assay,and CD4(+) and CD8(+) T cell detection were used for observation of humoral and cellular immune responses. Results:Immune sera strongly reacted with the expressed protein,antibody titer was up to 1:6400 as detected by *** blot analysis revealed a specific band at 38.3 *** the spleen cells of normal and immunized BALB/c mice were specifically stimulated with expressed protein,the optical densities were 0.12+/-0.03 and 0.34+/-0.04,*** latter were significantly higher than the former(Pcells were detected by using monoclonal antibody immunofluorscence *** results showed that the percentage of CD4(+) and CD8(+) T cells of immunized group were significantly higher than that of control group(Pcell-mediated immune responses and elevated NKC activity to products made with a eukaryotic expression sytem could be specifically detected in BALB/c *** findings indicated that the expressed protein could enhance the immune function in mice.

关键词： plasmodium falciparum circumsporozoite protein held cell expressed product immune response

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：