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heat stability of winter wheat depends on cultivars, timing and protective methods

Journal of Integrative Agriculture 2020年第8期19卷 1984-1997页

作者： LI Qiang CHANG Xu-hong MENG Xiang-hai LI Ding ZHAO Ming-hui SUN Shu-luan LI Hui-min QIAO Wen-chen Dryland Farming Institute Hebei Academy of Agriculture and Forestry SciencesHengshui 053000P.R.China Key Lab of Crop Drought Tolerance Research of Hebei Province Hengshui 053000P.R.China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China

heat stress negatively affects wheat production in many regions of the *** present,sensitivity to heat stress remains one of the least understood aspects of wheat genetics and breeding,and measures for preventing heat stress are *** this study,we used three cultivars of winter wheat(GY2018,SL02-1 and SY20)to evaluate the effect of heat stress at different days after anthesis(DAA)on yield and *** stability of the cultivars were analyzed and evaluated for the effects of two kinds of regulators on wheat under heat stress *** treatment at 7 DAA led to the most substantial reduction in yield while GY2018 had the best heat stability with respect to yield,and demonstrated the most positive effects on several quality traits including protein content,sedimentation volume and glutenin and gliadin *** treatment at 14 DAA had the least reduction in yield,while SY20 had the best heat stability with respect to yield and heat treatment had minimal effects on *** treatment at 21 DAA had only a limited effect on yield,while SL02-1 had the best heat stability with respect to yield,but it showed the most negative effects on *** time at 14 DAA and protein content at 21 DAA can be used as indicators for detecting the stability of quality under heat *** the three studied cultivars,SY20 was the most sensitive to heat stress with the stable time decreasing from 26.4 to 9.1 min,a higher sedimentation volume at 7 DAA,and a lowerγ-gliadin content which increased 2.4-fold under high-temperature *** addition of various regulators had different effects:potassium dihydrogen phosphate(KDP)was more protective of yield with heat stress at 7 DAA,while Duntianbao(DTB)had better effects on quality with heat stress at 21 DAA.

关键词： wheat high temperature heat stability yield quality prevention

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heat stability of HA Antigen in Influenza Virus Rapid Diagnosis Kit

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Animal Husbandry and Feed Science 2013年第1期5卷 20-21页

作者： TANG Jing MA Lei ZHOU Fang-ye SONG Shao-hui LIAO Guo-yang BAI Wei WANG Jun Institute of Medical Biology Chinese Academy of Medical Sciences Peking Union Medical College

[ Objective] The aim was to select protective agent of HA antigen of influenza virus. [ Method] H3N2 and H1 N1 subtype influenza virus were added into A - F groups to conduct accelerated test at 37 ℃and measure HA titer through hemagglutination test. t Result] Hemagglutination titers of H3 N2 and H, N1 subtype influenza virus were 20 and 32 in group A （ with PBS buffer solution） for 28 d; heat stability of HA antigen proved the best in group F （with BSA, fucose, proclin 300, triton 100） ; hemagglutination titers of HA antigen of H3N2 and H1 N1 subtype virus were 48 and 96 for 28 d. [ Conclusion] Components in group F were best in protection on HA antigen, which can be a candidate of protective agent.

关键词： Influenza virus HA antigen heat stability Protective agent

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Effect Mechanism of Structure-Changed Water on heat stability of Lysozyme

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Transactions of Tianjin University 2003年第3期9卷 177-179页

作者：赵林谭欣 School of Environmental Science and Engineering

Based on the mechanism of the effect of hydration on the heat stability of lysozyme and the theory of water molecule clusters, the effect of structure-changed water on heat stability of lysozyme has been studied. The results obtained by differential scanning calorimetry (DSC) showed that the thermal denaturation temperature of lysozyme had been elevated 8.47 K through hydration of lysozyme with processed water whose structure had been changed so it was called "structured water" compared to ordinary water. The reason is that structured water changed the dipole moment of water molecules and easily formed cyclic water hexamer or cage-like water hexamer, so that the interacting force of maintaining three-dimensional conformation of lysozyme could be reinforced.

关键词： lysozyme heat stability structured water water molecule clusters

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High-temperature dielectric and microwave absorption performances of TiB2/Al2O3 ceramics prepared by spark plasma sintering

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Science China(Technological Sciences) 2021年第6期64卷 1264-1275页

作者： LIU XiongZhang LUO Hui LI YinRui CHEN Fu LIU JieLing YANG WenTao WANG Xian GONG RongZhou School of Optical and Electronic Information Huazhong University of Science and TechnologyWuhan 430074China School of Information Science and Engineering Wuhan University of Science and TechnologyWuhan 430081China

TiB_(2)/Al2O3 ceramics reinforced with Mg O are prepared by spark plasma sintering(SPS).The dielectric and microwave(MW)absorption properties are *** results indicate that both the commercial TiB_(2)(C-TiB_(2))content and preparing temperature play important roles in the dielectric ***,TiB_(2)/Al2O3 composite shows the best MW absorption property when the C-TiB_(2)content and preparing temperature are 9 wt%and 1400°*** further improve the MW absorption properties,the composite containing 9 wt%synthesized TiB_(2)(S-TiB_(2))has been sintered at 1400°*** high-temperature complex permittivity is greater than that of TiB_(2)/Al2O3 composite with 9 wt%C-TiB_(2)sintered at 1400°C and is directly proportional to the ***,TiB_(2)/Al2O3 composite with 9 wt%S-TiB_(2)possesses a better MW absorption at 25–500°C,its effective absorption bandwidth(RLheat stability and considerable high-temperature MW absorption ability grant TiB_(2)/Al2O3 ceramics practical applications as high-temperature microwave absorption materials(MAMs).

关键词： titanium boride aluminium oxide heat stability electric conductivity high-temperature microwave absorption properties

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Synthesis and Characterization of Solid Electrolyte Ce_(0.95)Ca_(0.05)F_(2.95)

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Journal of Rare Earths 2004年第S1期22卷 103-105页

作者：王淑兰田芳张国良王常珍 School of Science School of Materials & Metallurgy Northeastern University Shenyang 110004 China

Polycrystalline Ce_(0.95)Ca_(0.05)F_(2.95) was prepared by a conventional solid-state reaction. The heat stability and ion conductivity of Ce_(0.95)Ca_(0.05)F_(2.95) were investigated by a high temperature calorimeter and an ac impedance technique. Experimental results indicate that Ce_(0.95)Ca_(0.05)F_(2.95) is stable at temperatures below 1000 K and represents the ionic conductivity of 1×10^(-3) to 10^(-1) Ω^(-1)·cm^(-1) in temperature range of 660 to 1180 K.

关键词： cerium fluoride solid electrolyte heat stability ion-conductivity rare earths

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Analysis of Biological Activity and Immunogenicity of Extracellular Products from Streptococcus iniae

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Agricultural Biotechnology 2018年第4期7卷 87-92页

作者： Danling GUO Shihui ZHOU Siyao ZHENG Dawei SONG Xuli ZHANG Huanying PANG Peiwen WU Jichang JIAN Yang HUANG College of Fisheries Guangdong Ocean UniversityZhanjiang 524088China National Demonstration Center for Experimental Aquaculture Science Education Zhanjiang 524088China Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals Zhanjiang 524088China Key Laboratory of Diseases Controlling for Aquatic Economic Animals of Guangdong Higher Education Institutions Zhanjiang 524088China

[Objectives] This study aimed to analyze the biological activity and immunogenicity of extracellular products from Streptococcus iniae .[Methods] S. iniae was incubated with brain heart infusion agar medium （BHIA ＋ 4% calf serum） for 60 h. The bacterial liquid was rinsed with PBS, centrifuged, and filtered through microporous filtering film to collect extracellular products （ECPs）.[Results] Extracellular proteinase （ECPase） of S. iniae exhibited amylase, protease, lecitinase, gelatinase, lipase activities and hemolytic activity but had no urease activity. EDTA, DTT and PMSF could reduce ECPase activity to 72.4%, 77.6% and 72.4%, respectively. Cu^2＋ , Ca 2＋ , K^＋ and Mg^2＋ exhibited an inhibitory effect on ECPase activity, whereas Fe^3＋ , Co^2＋ and Mn^2＋ could activate ECPase activity. ECPs had good heat stability and exhibited relatively high activities under alkaline conditions. The optimal temperature for ECPs was 55 ℃. Two-dimensional electrophoresis was performed to analyze the main protein of ECPs. The results indicated that there are 12 main bands of ECPs, and the molecular weights mainly ranged between 28-68 kDa. About 120 protein spots were detected, and the molecular weights mainly ranged between 26-95 kDa. The mouse anti- S. iniae was used for Western-blot analysis of ECPs, and the results showed that there were four proteins, with molecular weights of 26, 37, 95, and 97 kDa, respectively. The pathogenicity assay indicated that ECPs of S. iniae were highly pathogenic to tilapia. The mortality rate of tilapia was enhanced as the concentration of ECPs increased.[Conclusions] This study provided a certain theoretical basis for revealing the pathogenic mechanism of Streptococcus iniae .

关键词： Streptococcus iniae Extracellular products heat stability Mortality rate

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