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Molecular Detection of healthiness of Bombyx mori

Agricultural Science & Technology 2012年第11期13卷 2273-2277,2322页

作者：刘彬斌罗峰杜周和吴建梅陈义安刘俊凤四川农业科学院蚕业研究所四川南充637000 南充市农牧业局四川南充637000

[Objective] The paper was to explore the regularity between heat shock protein expression and the healthiness changes of Bombyx moil materials. [Method] The representative heat shock protein gene Bmhsp24.3 was screened by bioinfor- matic analysis method, and carried out real-time PCR expression analysis. [Result] The target gene Bmhsp24.3 expressed in different B. mori materials, but the expres- sion level in different materials significantly varied. The relative expression level of the gene had different degrees of changes under different rearing conditions. With the increase of rearing temperature, the gene expression was upregulated. The ma- terials with better healthiness had remarkable increase in expression of target gene, while the materials with poorer healthiness had less increase in expression of target gene. The expression difference of target gene Bmhsp24.3 was exactly consistent with the healthiness of breeds. [Conclusion] The healthiness of materials had rela- tionship with expression of target gene Bmhsp24.3. the higher the expression of tar- get gene Bmhsp24.3 was, the better the healthiness of materials was; conversely, the lower the expression of target gene Bmhsp24.3 was, the poorer the healthiness of materials was.

关键词： Bombyx mori Bmhsp24.3 healthiness Real-time PCR

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The mycobiota of discoloured needles of the Swiss stone pine(Pinus cembra L.) in the Tatra Mountains(Poland)

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Journal of Mountain Science 2018年第12期15卷 2614-2621页

作者： Wojciech PUSZ Anna BATURO-CIESNIEWSKA Tomasz ZWIJACZ-KOZICA Department of Plant Protection Diuision of Plant Pathology and MycologyWroclaw Uniuersity of Environmental and Life SciencesGrunwaldzki Sq.24aWroclaw50-363Poland Department of Biology and Plant Protection Division of Molecular Phytopathology and MycologyUniuersity of Science and TechnologyKordeckiego Str.2085-225BydgoszczPoland Tatra National Park Kuznice 134-500ZakopanePoland

The Swiss stone Pine(Pinus cembra L.) is an alpine species, fairly commonly occurring in the Alps and the Carpathians, close to the timberline. Natural sites of the Swiss stone pine in Poland are found exclusively in the Tatra Mountains, within the area of the Tatra National Park(TNP). In 2017, the health status of the needles of P. cembra was determined and their mycological analysis was carried out. As a result, 11 species of fungi were isolated from the needles showing disease symptoms, manifested by various shades of discoloration. The most frequently observed species was Fusarium oxysporum. It probably does not bring on disease symptoms, but may affect the development of other frequently isolated species(such as Sydowia polyspora, Lophodermium sp. and Lophodermium conigenum) that are pathogenic to Pinus spp.

关键词： Pathogenic fungi Swiss stone pine Tatra National Park plant pathology healthiness status of pine

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