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hantavirus Infection during Pregnancy

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Virologica Sinica 2021年第3期36卷 345-353页

作者： Deng-Hui Lu Hong Jiang Jian-Qi Lian The Center of Diagnosis and Treatment of Infectious Diseases The Second Affiliated HospitalAir Force Medical UniversityXi'an 710038China

hantavirus infection is a global health challenge,causing widespread public *** recent years,cases of hantavirus infection in pregnant women have been reported in many *** infected pregnant women and their fetuses appear to have more severe clinical symptoms and worse clinical ***,to study the prevalence of hantavirus infection in pregnant women,this study will focus on the epidemiological distribution of the virus,different virus species penetrating the placental barrier,and factors affecting the incidence and clinical outcome of the infection in pregnant women and their *** addition,this review will also discuss the diagnostic tools and treatments for pregnant patients and provide an overview of the relevant future research.

关键词： hantavirus Pregnancy Hantaan virus(HTNV) HPS HFRS

Stable Expression of hantavirus H8205 Strain G1/IL-2 Gene and Immune Protection of the Fusion Gene

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2007年第2期27卷 124-127页

作者：熊颖袁媛贾珉余冰黄汉菊 Department of Pathogenetic Biology School of Basic Medical Science Tongji Medical College Huazhong University of Science and Technology

To explore the feasibility of stable expression of hantavirus H8205 strain G1 segment and human IL-2 fusion gene in Vero cells, and to examine the immune protection effects on mice vaccinated with this recombinant eukaryotic expression vector containing hantavirus G1 gene and IL-2 gene. With the help of lipofectamine, the Vero cells were transfected with pcDNA3.1/HisB-IL-2-G1 and the positive cells were selected by G418. IFAT and SDS-PAGE elec- trophoresis were used to determine the stable transfection and expression of recombinant protein. Each mouse was inoculated with plasmids intramuscularly （i.m.） three times, 2 boosts were given at 2-week intervals, serum anti-hantavirus antibodies were detected by ELISA and neutralizing antibodies （NAb） were detected by Plaque Reduction Neutralization Test. The fusion protein expressed in Vero cells was 78 kD, corresponding to the estimated molecular size. The neutralizing antibody titers of mice with pcDNA3.1/HisB-IL-2-G1 were 1：20-1：80. IL-2/G1 fusion gene could be transferred in Vero cells and stably express the fusion protein. Specific humeral immune responses in mice can be induced with the recombinant eukaryotic expression vector containing the fusion gene, which lays the foundation for further development of therapeutic HTNV vaccine.

关键词： hantavirus fusion gene stable expression immune effect

Enhancing DNA vaccine potency against hantavirus by co-administration of interleukin-12 expression vector as a genetic adjuvant

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Chinese Medical Journal 2005年第4期118卷 313-319页

作者： ZHENGLan-yan MOULing LINSong LURun-ming LUOEn-jie DepartmentofMicrobiologyandParasitology ChinaMedicalUniversityShenyang110001China DepartmentofLaboratoryMedicine MunicipalHospitalofInfectiousDiseaseShenyang110003China

Background The heavy incidence and mortality of hemorrhagic fever with renal syndrome, as well as no specific drugs in curing the disease, clearly indicate the need for development Of the more effective hantavirus vaccine. Refining the DNA vaccination strategy to elicit more clinically efficacious immune responses is now under intensive investigation. In the present study, we examined the effects of using an interleukin-12 expression plasmid as a genetic adjuvant to enhance the immune responses induced by a DNA vaccine based on the S gene encoding nucleocapsid protein against hantavirus. Methods BALB/c mice were immunized three times by intramuscular inoculations of DNA vaccine encoding of hantanvirus nucleoeapsid protein alone or in combination with a plasmid expressing murine interleukin-12 (pcIL-12). Booster immunizations were employed 2 times at 2-week interval. To evaluate the Immoral and cellular immune responses, antigen-specific lymphocyte proliferation and antibody production were assayed by MTT method and ELISA respectively. The level of interleukin-4 and interferon-gamma in the splenic lymphocytic cultured supernatant were detected with ELISA kit at day 5, 10, 17, 35 and 42 after primary immunization. Results Antigen-specific IgG antibodies was increased markedly at day 17 in the experiment groups and reached a plateau after day 35. As pcIL-12 co-injected, a significant inhibition of antigen-specific IgG levels was displayed over the period and the antibody mean titre was decreased to only about 1: 50 at day 42 after primary immunization, significantly lower than the group immunized with pcDNA3.1 + S alone, in which the mean titre was about 1:70. Interferon-gamma was increased remarkably by the co-injection of pcIL-12 compared with the injection of pcDNA3.1 + S alone. However, the production of interleukin-4 was inhibited by pcIL-12 coinjection. Furthermore, pcIL-12 co-injection efficiently enhanced antigen-specific lymphocyte proliferation. Conclusion Hu

关键词： DNA vaccines hantavirus gene adjuvant interleukin-12 nucleocapsid proteins

Spatial structure of rodent populations and infection patterns of hantavirus in seven villages of Shandong Province from February 2006 to January 2007

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Chinese Medical Journal 2011年第11期124卷 1639-1646页

作者： SUN Lin SHAO Qi WANG Zhi-qiang KANG Dian-min LI Shi-wei LI Xue-gang XUE Fu-zhong WANG Jie-zhen Department of Epidemiology and Biostatistics School of PublicHealth Shandong University Jinan Shandong 250012 China Qingdao Health Inspection Bureau Qingdao Shandong 266034China Junan County Center for Disease Control and Prevention JunanLinyi Shandong 276600 China

Background Hemorrhagic fever with renal syndrome （HFRS） is endemic in Junan county, Shandong Province, China. We conducted geographic information system （GIS）-based spatial analysis with the objective of estimating the spatial distribution of rodent populations and their hantavirus infection patterns, to describe the spatial relationships of hantavirus strains in small ecological areas and to identify key areas in endemic areas of HFRS for future public health planning and resource allocation.

关键词： rodent population hantavirus small ecological areas spatial analysis

First Evidence of hantavirus in Central Iran as an Emerging Viral Disease

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Advances in Infectious Diseases 2014年第4期4卷 173-177页

作者： Sadegh Chinikar Abbas Ali Javadi Arash Hajiannia Behroz Ataei Tahmineh Jalali Sahar Khakifirouz Norbert Nowotny Jonas Schmidt-Chanasit Nariman Shahhosseini Arboviruses and Viral Haemorrhagic Fevers Laboratory (National Ref. Lab) Department of Virology Pasteur Institute of Iran Tehran Iran Infectious Diseases and Tropical Medicine Research Center Isfahan University of Medical Sciences Isfahan Iran Institute of Virology University of Veterinary Medicine Vienna Austria Department of Microbiology and Immunology College of Medicine and Health Sciences Sultan Qaboos University Muscat Oman WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research Department of Virology Bernhard Nocht Institute for Tropical Medicine Hamburg Germany

hantavirus is a zoonosis transmitted from rodents to humans. Asymptomatic infected rodents can secrete hantaviruses in the urine, feces, and saliva. The main route of infection transmission to human is aerosols contaminated with the virus. This study was designed to evaluate the serological and molecular prevalence of hantavirus as an emerging zoonoses disease among street sweepers in Isfahan province, central Iran. Serum samples from 200 street sweepers in healthy condition and those with recent renal failure were tested by ELISA (IgM and IgG). Molecular analysis was subsequently applied for IgM positive cases. From these samples, 9 (4.5%) were positive, of which 2 (22.22%) were positive for both IgM and RT-PCR, while 7 (77.77%) were positive for IgG. The mean age and work experience of the positive cases were 39.7 and 11.5 respectively. According to our observations, all positive cases reported prevalence of rodents in their work place. The logistic regression test showed that the age and work experience were not risk factors for being positive, but prevalence of rodents in work place was a risk factor for being positive, when compared with negative cases. This is the first comprehensive study on the prevalence of hantavirus with positive results coming from Iranian population, which can raise the public awareness for the hantavirus infections as a public health threat.

关键词： hantavirus Emergence Zoonoses Rodent Arboviruses Iran

Preparation　and　antigenic　characterization　of　rat　monoclonal　antibodies　againts　hantavirus

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Journal of Medical Colleges of PLA(China) 1995年第4期10卷 248-252,273页

作者：徐海峰刘原梁喆米力崔龙洙杨为松

Monoclonal antibodies (McAb) to hantavirus (HTV)were derived from the hybridoma fused with Lou/c plasmocytoma rat cells (IR983F) and spleen cells from lou/c inbred rat immunized with HTV Chen strain. Eleven cell lines... 详细信息

关键词： hantavirus monoclonal antibody antigenic analysis structural protein

DNA vaccine encoding hantavirus Gn, targeted to MIIC by trafficking molecule LAMP, significantly enhances specific immune responses

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DNA vaccine encoding Hantavirus Gn, targeted to MIIC by traf...

Rats(Rattus norvegicus and losea) harboring Seoul hantavirus in southern China

第十届全国免疫学学术大会

作者： Jinpeng Zhang Shaobo Xiao Peihan He Yunwei Wang Dongbo Jiang Kun Yang Department of Immunology the Fourth Military Medical University Brigade of Cadet the Fourth Military Medical University

Lysosome-associated membrane protein(LAMP)can target and bind to endosome/lysosome,one of the most important components of the MHC class II-processing compartment(MIIC)in the exogenous antigen-processing *** trafficking greatly enhance the immune response because endogenous antigen can take advantage of LAMP being directly carried into MIIC,activating CD4+helper T cells for effective immue response and long-term immune *** glycoprotein N-terminal,named Gn,could induce neutralizing antibody production with a low serum titer as natural *** analyze the influence of LAMP on Hantaan virus(HTNV)Gn vaccine potency and develop a novel effective vaccine against HTNV,we constructed three eukaryotic vectors as naked DNA vaccine named p VAX-Gn,p VAX-LAMP and p VAX-LAMP/Gn,***/c mice were immunized with those plasmids,the specific humoral and cellular responses elicited against HTNV Gn were measured by ELISA,cytotoxicity assays and ELISPOT assay(IFN-γ).To measure the protective efficacy,virus challenging in vivo and neutralizing antibody valence were conducted by viral load detection(q RT-PCR and sandwich ELISA)and the cell microculture neutralization *** found that HTNV Gn showed a strong immunogenicity to elicit both humoral and cellular responses with LAMP as a *** with the current prophylactic inactive vaccine,p VAX-LAMP/Gn showed a stronger cellular *** worth raising,a significant long term memory reponse was observed only from the group of p VAXLAMP/*** analysis by HE staining demonstrated that p VAX-LAMP/Gn was not *** of protection assay in vivo indicated that the immune response established was HTNV specific and *** findings not only demonstrated that the LAMP as a trafficking molecule can introduce Gn to MHCII presenting pathway and significantly enhanced HTNV specific immune response,but also suggested that the p VAX-LAMP/Gn as a DNA vaccine had potentia

关键词： DNA vaccine hantavirus LAMP targeting Immune responses

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Rats(Rattus norvegicus and losea) harboring Seoul hantavirus...

The recombinant adenoviruses containning chimeric genes and incorporated with CTL epitopes and HSP70 C-terminal could induce specific immune responses against hantavirus

2015新发传染病防治热点研讨会

作者： Ping Huang Guangdong Provincial Center for Disease Control and Prevention

The hemorrhagic fever with renal syndrome(HFRS) as a zoonotic disease threatens public health and its incidence increased sharply for present years in southern China. A survey of HFRS including in both natural hosts and human was conducted in Qingyuan, southern China, during 2011- 2013. It was found that one, two and seven confirmed cases of HFRS occurred in 2011, 2012 and 2013, respectively. Rodent densities ranged between 1.73%-12.2% and the Seoul hantaviruses only in both Rattus norvegicus and losea were detected by RT-PCR. The positive ratios in human were serologically 0.95% and 0.94% with IIFA in 2011 and 2013, respectively. Both fragments detected from Rattus were highly homologically with those of Seoul hantavirus HB55(96.2%) and L99(95.1%), respectively. It was concluded that HFRS is becoming an emerging and dangerous disease in southern China and it is necessary to further perform molecular characterization of strains isolated from rodents and humans.

关键词： Hemorrhagic fever with renal syndrome(HFRS) hantavirus Rattus Survey Southern China

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The recombinant adenoviruses containning chimeric genes and ...