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Standardization and Identification of Minor Components of Silymarin (MK-001)

Chinese Herbal Medicines 2012年第3期4卷 237-244页

作者： Yan-ze LIU[a][b] David Yue-wei LEE[a] Bioorganic and Natural Product Laboratory McLean Hospital/Harvard Medical SchoolMA 02478USA Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100193China

Objective To develop a highly effective hplc method for standardization of silymarin and for characterization of minor components, which has not been reported previously. Methods An hplc fingerprinting method and 1 H-NMR spectroscopy were employed to provide assurance for a standardized silymarin product (MK-001). Results A total of 18 marker compounds were identified and characterized including ten flavonolignans and eight small molecules including adenine, adenosine, uridine, and 3,5,7-trihydroxychromone which were first isolated and characterized from silymarin. The hplc fingerprinting method and 1 H-NMR had been established for complete assignments of hplc peaks with intensity over 1.0%. Conclusion The successfully established hplc fingerprinting method and 1 H-NMR could be applied for the standardization of commercial silymarin products. MK-001 represents the first standardized silymarin with the highest content of flavonolignans ( > 90%).

关键词： adenine flavonolignans 1 H-NMR hplc fingerprinting silymarin

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Effects of Postharvest Processing and Geographical Source on Phytochemical Variation of Corydalis Rhizoma

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Chinese Herbal Medicines 2013年第2期5卷 151-157页

作者： ZHAO Yun-peng LI Jing-hui YANG Shu-ting FAN Jie FU Cheng-xin Key Laboratory of Conservation Biology for Endangered Wildlife Ministry of EducationCollege of Life SciencesZhejiang University Laboratory of Systematic & Evolutionary Botany and Biodiversity Institute of Ecology and Conservation Center for GeneResources of Endangered WildlifeZhejiang University

Objective To assess the relative contributions of postharvest processing and geographical source to phytochemical variation of Corydalis Rhizoma, and rhizome of Corydalis yanhusuo, and to examine what phytochemical components are the most sensitive to the differences of each factor and how they change. Methods hplc fingerprinting and LC-MS coupled with chemometric approaches were applied. Results The results of principal component analysis(PCA) and hierarchical cluster analysis(HCA) explicitly demonstrated the postharvest processing could produce a greater impact on the phytochemical profiles of Corydalis Rhizoma than geographical source. The contents of most compounds increased after water boiling while decreased after ***, coptisine, and palmatine were the most variable components in processing. Geographical sources also led to a remarkable phytochemical differentiation, in which the environmental variation of the three regions might play a role. Dehydrocorybulbine, coptisine, dehydrocorydaline, and protopine varied most among the three production regions and decreased sequentially in Zhejiang, Shaanxi, and Jiangsu provinces, China. Conclusion Both postharvest processing and geographical source should be enhanced with the priority for the former in the quality control of Corydalis Rhizoma. The application of boiling is supported but the consistency should be improved in practice. Sulphur-fumigation is strongly suggested to be abandoned.

关键词： chemometrics Corydalis yanhusuo geographical source hplc fingerprinting phytochemical variation postharvest processing

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