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Inducible overexpression of porcine homeobox A10 in the endometrium of transgenic mice

Journal of Integrative Agriculture 2016年第6期15卷 1338-1344页

作者： LIN Rui-yi WU Di ZHAO Chang-zhi CHEN Shang-shang XIAO Qian LI Xin-yun ZHAO Shu-hong Key Lab of Agricultural Animal Genetics Breeding and Reproduction Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production Huazhong Agricultural University Wuhan 430070 P.R China College of Animal Science and Technology Northeast Agricultural University Harbin 150030 P.R.China

Homeobox A10 （hoxa 10） is a well-known transcription factor that plays an important role in directing endometrial differ- entiation and establishing the conditions required for implantation. Interestingly, the expression level of hoxaIO may be associated with litter size. To study the effects of the porcine hoxaIO promoter fragment on the expression of hoxaIO gene in vivo, we generated a transgenic mouse model using pronuclear microinjection, and measured the expression of hoxaIO in the endometrium. There was no difference in the expression level of hoxaIO between transgenic and wild- type mice in the absence of hormone stimulation. However, following treatment with progesterone and estradiol benzoate, the expression level of hoxaIO was significantly increased in transgenic mice compared with that of wild-type mice. Fur- thermore, the litter size of transgenic females was larger than that of wild-type females （7.02±1.73 vs. 6.48＋1.85; P=0.14）. Moreover, the difference of litter size was greater in the later parities （7.33±1.62 vs. 6.37±2.02; P=0.08） compared with the first parity （6.76±1.81 vs. 6.61v1.67; P=0.77） between transgenic and wild-type mice. Therefore, our transgenic mouse model provides exciting insights regarding the actions of hoxaIO and its hormone-inducible promoter in vivo. The present study offers valuable proof of principle to develop transgenic pigs with a hormone-inducible promoter regulating hoxaIO to alter litter size.

关键词： porcine hoxa 10 transgenic mice endometrium

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Expression and mechanism of action of miR-196a in epithelial ovarian cancer

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Asian Pacific Journal of Tropical Medicine 2016年第11期9卷 1082-1087页

作者： Bo Yang Sheng-Ze Li Ling Ma Hong-Li Liu Jian Liu Jun-Jun Shao Department of Female Tumor First Affiliated Hospital of Bengbu Medical College

Objective:To explore the expression,biological function and possible mechanism of action of microRNA molecular-196a(miR-196a) in epithelial ovarian ***:RT-PCR was used to detect the expression quantities of epithelial ovarian tissue,benign ovarian tissue,normal ovary epithelial tissue,ovarian cancer cell lines and miR-196 a in normal ovarian epithelial cells to analyze the relationship between the expression of miR-196 a and the clinical pathologic parameters of ovarian *** those cell lines,the cell line of which miR-196 a expressed the most or least was selected and transfected the ovarian cancer cell line by using negative control plasma and miR-196 a *** transfection,RT-PCR was used to test the expression quantity of miR-196 a,Transwell chamber method was applied to determine the migration and invasion abilities of ovarian carcinoma cells and Western blot was employed to detect the expression of hoxa10 ***:The relative expression quantities of miR-196 a in ovarian cancer tissue and benign ovarian tissue were significantly higher than that in normal ovarian epithelial tissue,and the expression quantity of miR-196 a in ovarian cancer tissue was distinctively higher than that in benign ovarian tissue(P 0.05).Compared with normal ovarian epithelial cell line IOSE80,the expression quantities of miR-196 a of all ovarian cancer cell lines increased obviously and differences were statistically significant(P < 0.05).Among them,the expression of miR-196 a of ovarian cancer cell line SKOV3 was the highest,while it decreased significantly(4.678 ± 0.785 vs.2.131 ± 0.345,t = 2.938,P < 0.

关键词： MiR-196a Epithelial ovarian cancer Migration Invasion hoxa 10

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