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Experimental study of gemcitabine combination with radiation in vitro on a highly metastatic human ovarian cancer cell line ho-8910pm

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The Chinese-German Journal of Clinical Oncology 2010年第11期9卷 648-651页

作者： Linhui Gu Lijuan Qian Chihong Zhu Yutian Ling hongwei Zhang, Xianglin Liu Department of Cancer Research Zhejiang Cancer Hospital Hangzhou 310022 China

Objective： The aim of the study was to investigate the mechanism of gemcitabine （GEM） combination with radiation on the high metastasis human ovarian cancer cell line （ho-8910pm）. Methods： Human ovarian cancer cell line ho- 8910pm was treated with different concentrations of gemcitabine for 24 h, then the cells were counted. In the study of GEM combination with radiation, an efficiency of colony formation was observed; the cell cycle and apoptosis were analyzed by flow cytometry; the experiment of depend on the time and its radio sensitivity were observed by using mitotic index with the cells for each 24, 48, 72 and 96 h after experiment. Results： It suggested that the GEM had an inhibition effect on the human ovarian cancer cell line. The alive cell numbers were decreased by following a height of GEM concentration. When GEM in combina- tion with a radiation, the suppression was significantly increased than that of single GEM therapy. The efficiency of colony formation was significantly lower, under this condition the cell could be arrested at G0-G1 phase and could be decreased to enter into the S phase; the apoptosis percentage could be significantly increased; especially, under the 4 Gy and 6 Gy doses the cell apoptosis was more obvious. GEM combination with radiation had depended on the time to the cells; mitotic index of the calls in combination group was observed significantly lower than that of single GEM therapy or single radiation, and this showed that it had an effect of radiosensitivity. Conclusion： The GEM has a significant growth inhibition on the human ovarian cancer cells, GEM combination with radiation could induce ho-8910pm cell occurred arrested and apoptosis. It has depended on the time and has a radiosensitivity effect. The result shows that it is a better method to treat the human ovarian cancer by using radiotherapy combined with gemcitabine.

关键词： gemcitabine (GEM) radiation human ovarian cancer ho-8910pm cell line

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