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hg-induced sevs Mediate Biomechanics of HK-2 Cells

Nanomanufacturing and Metrology 2023年第4期6卷 81-91页

作者： Fan Yang Jiajia Wang Tuoyu Ju Shuwei Wang Kaige Qu Zhengxun Song Yujuan Chen Zuobin Wang International Research Centre for Nano Handling and Manufacturing of China Changchun University of Science and TechnologyChangchun 130022China Centre for Opto/Bio-Nano Measurement and Manufacturing Zhongshan Institute of Changchun University of Science and TechnologyZhongshan 528437China Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing Changchun University of Science and TechnologyChangchun 130022China Afliated Hospital Jilin Medical UniversityJilin 132013China JR3CN&IRAC University of BedfordshireLuton LU13JUUK

Small extracellular vesicles (sevs) participate in the pathological progression of high glucose (hg)-induced kidney injury, which is closely related to diabetic nephropathy. How sevs specifically mediate the cell biomechanics underlying hg injury is unclear. Herein, we utilized a versatile atomic force microscope to determine the contributions of sevs in hg-induced cellular injury. The sevs extracted from the culture medium of human proximal tubule kidney (HK-2) cells treated by hg for 72 h (hg-induced sevs) were verified and analyzed by multiple techniques, and the results indicated the effective production and the effect of dehydration on the shape of hg-induced sevs. Further investigation on the morphologies of HK-2 cells treated by hg-induced sevs showed that the surface roughness of the HK-2 cells increased, and their pseudopodia transitioned from lamellipodia to filopodia, with almost doubled mean pseudopodia length. Quantitative analysis of the mechanical responses of the cells revealed that the mean Young’s modulus increased by 26.2%, and the mean adhesion decreased by 36.8%. The indirect mediation of cellular biomechanics guided by hg-induced sevs was evaluated by comparing it with previously studied direct hg injury. The hg-induced sevs caused a greater reduction in cell adhesion and an increase in Young’s modulus compared with direct hg stimulation. This work suggested the ability of hg-induced sevs to elicit specific biomechanical responses during hg injury, advancing the understanding of the injury mechanism caused by hg. The comparison of the cellular biomechanics between direct and indirect hg stimulations through hg-induced sevs can be beneficial for the diagnosis and treatment of kidney injury.

关键词： AFM Cellular biomechanics hg-induced sevs hg HK-2 cells

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